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| 1 | A novel gene delivery system targeting cells expressing VEGF receptors显示文摘Two ligand oligopeptides GV1 and GV2 were designed according to the putative binding region of VEGF to its receptors. GV1, GV2 and endosome releasing oligopeptide HA20 were conjugated with poly-L-lysine or protamine and the resulting conjugates could interact with DNA in a noncovalent bond to form a complex. Using pSV2-β-galactosidase as a reporter gene, it has been demonstrated that exogenous gene was transferred into bovine aortic arch-derived endothelial cells (ABAE) andhuman malignant melanoma cell lines (A375) in vitro. In vivo experiments, exogenous gene was transferred into tumor vascular endothelial cells and tumor cells of subcutaneously transplanted human colon cancer LOVO, human malignant melanoma A375 and human hepatoma graft in nude mice. This system could also target gene to intrahepatically transplanted human hepatoma injected via portal vein in nude mice. These results are correlated with theGene delivery system targeting VEGF receptors relevant receptors (flt-1, flk-1/KDR) expression on the targeted cells and tissues. | LI JUN MIN JUN SONG HAN YI HUANG PEI KUN TIAN SHU MIN QU MIN YAO HUI QIU JIANG DA FANG WAN JING CHU LUO CHENG XIAO GU JIAN REN GU( National Labomtory for Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032,China)(National Laboratory of | 1999 | Cell Research1999，9，1： | 22 |
| 2 | Efficient Gene Targeting in Zebrafish Mediated by a Zebrafish-Codon-Optimized Cas9 and Evaluation of Off-Targeting Effect显示文摘Targeted genome modifications with the Cas9/gRNA system derived from the clustered regularly interspaced short palindromic repeat/CRISPR-associated(CRISPR/Cas)system have been successfully used in cultured human cells as well as in most model organisms,including zebrafish(Danio rerio),mouse,and fruit fly(Chang et al.,2013;Cong et al.,2013; | Da Liu Zhanxiang Wang An Xiao Yutian Zhang Wenyuan Li Yao Zu Shaohua Yao Shuo Lin Bo Zhang | 2014 | Journal of Genetics and Genomics2014，41，1： | 18 |
| 3 | Astragalus mongholicus ameliorates renal fibrosis by modulating HGF and TGF-β in rats with unilateral ureteral obstruction显示文摘Astragalus mongholicus(AM) derived from the dry root of Astragalus membranaceus Bge.var.mongolicus(Bge.) Hsiao is a widely used traditional Chinese medicine.The present study investigated the potential role of AM on renal fibrosis on a rat model of unilateral ureteral obstruction(UUO).We divided 48 Sprague-Dawley rats randomly into 4 groups:sham-operated group(Sham),untreated UUO group,AM-treated(10 g/(kg·d)) UUO group,and losartan-treated(20 mg/(kg·d)) UUO group as positive control.Haematoxylin & eosin(HE) and Masson staining were used to study the dynamic histological changes of the kidneys 7 and 14 d after operation.The expressions of fibronectin(FN),type I collagen(colI),hepatocyte growth factor(HGF),transforming growth factor-β1(TGF-β1),and α-smooth muscle actin(α-SMA) were analyzed by real-time polymerase chain reaction(PCR),immunohistochemistry staining,and Western blot.Results show that,similar to losartan,AM alleviated the renal damage and decreased the deposition of FN and colI from UUO by reducing the expressions of TGF-β1 and α-SMA(P<0.05),whereas HGF increased greatly with AM treatment(P<0.05).Our findings reveal that AM could retard the progression of renal fibrosis.The renoprotective effect of AM might be related to inhibition of myofibroblast activation,inducing of HGF and reducing of TGF-β1 expression. | Chuan ZUO Xi-sheng XIE Hong-yu QIU Yao DENG Da ZHU Jun-ming FAN | 2009 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2009，10，5： | 15 |
| 4 | Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis显示文摘骨头转移从疾病是乳癌的经常的复杂并发症和病态和死亡的一个普通原因。在转移期间,分泌蛋白质在在癌症房间和主人基质之间的相互作用起关键作用。为了描绘分泌蛋白质,那与乳癌骨头转移被联系,我们比较四个 MDA-MB-231 (MDA231 ) 衍生物房间的 secretomes 的没有标签的 proteomic 分析与骨头转移的改变的能力衬里的 preformed。128 蛋白质的一个总数被发现是一致地在骨头回归线癌症房间的调节媒介的 up-/down-regulated。改变的蛋白质的充实的分子的函数包括了受体绑定和 peptidase 抑制。通过乳癌房间的另外的 transcriptomic 分析,我们选择了 cystatin E/M (CST6 ) ,在骨头变形的房间下面调整的一个半胱氨酸朊酶禁止者,为进一步功能的研究。我们的结果证明 CST6 压制了乳癌房间的增长,殖民地形成,迁居和侵略。对癌症房间活动性的镇压功能被癌症执行导出房间的可溶的 CST6。更重要地,在癌症房间的 CST6 的宫外的表示在动物学习救了老鼠免于公开 osteolytic 转移和死亡,当时 CST6 击倒的显著地提高的癌症房间骨头转移和弄短的动物幸存。总的来说,我们的学习提供了乳癌骨头向性的全身的 secretome 分析并且作为乳癌 osteolytic 转移的真正的 suppressor 建立了分泌 CST6。 | Lei Jin Yan Zhang Hui Li Ling Yao Da Fu Xuebiao Yao Lisa X Xu Xiaofang Hu Guohong Hu | 2012 | Cell Research2012，22，9： | 15 |
| 5 | 二期尿道成型术更适合于需要横断尿道板并伴有严重弯曲畸形的近段型尿道下裂显示文摘对于伴有严重阴茎弯曲的尿道下裂,横断尿道板后是选择一期还是二期手术仍存在争议。这项回顾性研究比较评价了这两种方法的优劣。本研究共收集66例伴有严重阴茎弯曲的近端型尿道下裂的患者,根据手术方式分成两组,其中32例行一期手术(Duckett),34例行二期手术。一期和二期手术组的中位年龄分别为7.5岁和11.0岁,中位随访时间分别为28.5个月和35个月。所有患者术后尿道外口均位于龟头顶端。随访期间无患者出现阴茎弯曲复发,患者及其家属对阴茎的长度和外观均满意。术后两组各有8例患者出现并发症,两组间并发症率无统计学差异。一期手术尿道狭窄的远期并发症率高j(18.75%1,JO%)。在一期手术组中,青春期前患者的并发症率显著低于青春期后患者(10.52%w46.15%3。中期随访结果提示,横断尿道板可有效矫正近端型尿道下裂合并的重度阴茎弯曲。考虑到一期手术组中较高的尿道狭窄发生率,本文认为分期手术更适合伴有严重阴茎弯曲需要横断尿道板的近端型尿道下裂。 | Da-Chao Zheng Hai-Jun Yao Zhi-Kang Cai Jun Da Qi Chen Yan-Bo Chen Ke Zhang Ming-Xi Xu Mu-Jun Lu Zhong Wang | 2015 | Asian Journal of Andrology2015，17，1： | 13 |
| 6 | A feasible,economical,and accurate analytical method for simultaneous determination of six alkaloid markers in Aconiti Lateralis Radix Praeparata from different manufacturing sources and processing ways显示文摘Aconiti Lateralis Radix Praeparata(Fuzi) is a commonly used traditional Chinese medicine in clinic for its potency in restoring yang and rescuing from collapse. Aconiti alkaloids, mainly including monoester-diterpenoidaconitines(MDAs) and diester-diterpenoidaconitines(DDAs), are considered to act as both bioactive and toxic constituents. In the present study, a feasible, economical, and accurate HPLC method for simultaneous determination of six alkaloid markers using the Single Standard for Determination of Multi-Components(SSDMC) method was developed and fully validated. Benzoylmesaconine was used as the unique reference standard. This method was proven as accurate(recovery varying between 97.5%-101.8%, RSD < 3%), precise(RSD 0.63%-2.05%), and linear(R > 0.999 9) over the concentration ranges, and subsequently applied to quantitative evaluation of 62 batches of samples, among which 45 batches were from good manufacturing practice(GMP) facilities and 17 batches from the drug market. The contents were then analyzed by principal component analysis(PCA) and homogeneity test. The present study provided valuable information for improving the quality standard of Aconiti Lateralis Radix Praeparata. The developed method also has the potential in analysis of other Aconitum species, such as Aconitum carmichaelii(prepared parent root) and Aconitum kusnezoffii(prepared root). | ZHANG Yi-Bei DA Juan ZHANG Jing-Xian LI Shang-Rong CHEN Xin LONG Hua-Li WANG Qiu-Rong CAI Lu-Ying YAO Shuai HOU Jin-Jun WU Wan-Ying GUO De-An | 2017 | Chinese Journal of Natural Medicines2017，15，4： | 10 |
| 7 | H2 strain attenuated live hepatitis A vaccines:protective efficacy in a hepatitis A outbreak显示文摘AIM To investigate the protective efficacy ofH2 strain attenuated live hepatitis A vaccines(H2-strain vaccines)in hepatitis A(HA)outbreaks.METHODS With the permission of theirparents,5551 pre-school and grade 1-3 primaryschool children were inoculated with 1 dose(106.5TCID50)of H2-strain vaccines in anonrandomized,controlled trial conducted inFucheng County,Hebei Province in May 1997.Another 6485 children in the same grades andcompatible in gender and age were enrolled ascontrols.Epidemiological and serological surveywas conducted to evaluate the protectiveefficacy of the vaccines.ELISA was used todetect serum IgM anti-HAV.RESULTS HA outbreak started in early May1998,peaked in the middle of the same month,and lasted about 80 days.Overall 302 HA caseswere found,192(53.58%)were 5-9 years old.One vaccinee and 25 control cases were found tohave hepatitis A,which account for 0.28%(1/356)and 5.92%(25/422)of all vaccinees andcontrols in the 14 villages,respectively.The protective efficacy of vaccines was 95.27%(95% Cl:85.83%-104.72%).In subjects testedfor anti-HAV IgM from 13 villages,1(0.40%)overt and 11(4.06%)asymptomatic HAV caseswere found in 271 vaccinees,but 21(6.69%)ofovert and asymptomatic ones were found in 314controls.CONCLUSION H2 strain vaccines were excellent in preventing overt hepatitis A, but not so effective in preventing asymptomatic hepatitis A virus infection. A booster dose might be needed to get permanent reliable immunity. | Yu Liang Zhao Zong Da Meng Zhi Yi Xu Jun Jie Guo Shao Ai Chai Cheng Gang Duo Xuan Yi Wang Jin Feng Yao Hong Bin Liu Shun Xiang Qi Hui Bin Zhu | 2000 | World Journal of Gastroenterology2000，6，6： | 7 |
| 8 | Expression of Aspergillus niger 9891 Endoinulinase in Pichia pastoris显示文摘An endoinulinase gene from Aspergillus niger 9891 (CGMCC0991) has been expressed in Pichia pastoris GS115 using pPIC9 vector. The recombinant endoinulinase was highly expressed and the optimization of the expression in a 7 liter of fermentor has been investigated. In fermented broth, the concentration of protein secreted is 2.15 mg/ml. The activity of endoinulinase is 1501 U/ml with sucrose as substrate and 291 U/ml with inulin as substrate, 105 and 273 times higher than that from the original strain respectively. | 王建华 TENG Da YAO Yi Yang Yalin Zhang Fan | 2004 | High Technology Letters2004，10，4： | 6 |
| 9 | Advances in lithium niobate photonics:development status and perspectives显示文摘Lithium niobate(LN)has experienced significant developments during past decades due to its versatile properties,especially its large electro-optic(EO)coefficient.For example,bulk LN-based modulators with high speeds and a superior linearity are widely used in typical fiber-optic communication systems.However,with everincreasing demands for signal transmission capacity,the high power and large size of bulk LN-based devices pose great challenges,especially when one of its counterparts,integrated silicon photonics,has experienced dramatic developments in recent decades.Not long ago,high-quality thin-film LN on insulator(LNOI)became commercially available,which has paved the way for integrated LN photonics and opened a hot research area of LN photonics devices.LNOI allows a large refractive index contrast,thus light can be confined within a more compact structure.Together with other properties of LN,such as nonlinear/acousto-optic/pyroelectric effects,various kinds of high-performance integrated LN devices can be demonstrated.A comprehensive summary of advances in LN photonics is provided.As LN photonics has experienced several decades of development,our review includes some of the typical bulk LN devices as well as recently developed thin film LN devices.In this way,readers may be inspired by a complete picture of the evolution of this technology.We first introduce the basic material properties of LN and several key processing technologies for fabricating photonics devices.After that,various kinds of functional devices based on different effects are summarized.Finally,we give a short summary and perspective of LN photonics.We hope this review can give readers more insight into recent advances in LN photonics and contribute to the further development of LN related research. | Guanyu Chen Nanxi Li Jun Da Ng Hong-Lin Lin Yanyan Zhou Yuan Hsing Fu Lennon Yao Ting Lee Yu Yu Ai-Qun Liu Aaron J.Danner | 2022 | Advanced Photonics2022，4，3： | 6 |
| 10 | Functional analysis of two Sp1/Sp3 binding sites in murine Nanog gene promoter显示文摘Nanog 基因在维持 ES 房间和早胚胎的房间的 pluripotency 起一个关键作用。Nanog 基因的 A 5' 胁腹顺序被报导了在 Nanog 倡导者以内是调整差别,和二个规章的元素,也就是 Oct-4 和 Sox-2 绑定地点,被识别了调整 Nanog 的 transcriptional 活动基因。在这份报告,我们识别了在鼠科的 Nanog 基因抄写的规定位于 Nanog 基因 5'-flanking 区域的二个通常认为的 Sp1 有约束力的地点的角色。变化研究证明二个地点为 Nanog 倡导者活动是必要的。胶化移动和超级移动分析证明两个地点明确地绑 Sp1 和 Sp3。而且,显著地在主导否定的 Sp1 或 Sp3 异种的表示上禁止 Nanog 倡导者活动。这些结果建议抄写因素 Sp1 和 Sp3 为鼠科的 Nanog 基因表示是重要的。 | Da Yong Wu Zhen Yao | 2006 | Cell Research2006，16，3： | 5 |
| 11 | 经自然腔道取标本手术(NOSES)治疗结直肠癌的国际共识显示文摘近年来,经自然腔道取标本手术(NOSES)治疗结直肠癌已引起了广泛关注。NOSES应用于结直肠手术的潜在优势包括减轻术后疼痛,减少伤口并发症,减少对术后镇痛的需求,加快肠道功能的恢复,缩短住院时间,更好的美容效果,以及给患者带来了更佳的心理状态。尽管NOSES的手术创伤显著减轻,然而该技术也存在一些潜在问题。尤其随着这项新技术的兴起,人们对其无菌的把控、肿瘤学结果和病例选择等问题表示出关切。因此,迫切需要一部操作指南来规范NOSES手术在结直肠癌中的开展。经过国际NOSES联盟全体成员的三轮讨论,最终形成了该部共识。这一共识有助于NOSES在全球范围内的长远发展。 | Xu Guan Zheng Liu Antonio Longo Jian-Chun Cai William Tzu-Liang Chen Lu-Chuan Chen Ho-Kyung Chun Joaquim Manuel da Costa Pereira Sergey Efetov Ricardo Escalante Qing-Si He Jun-Hong Hu Cuneyt Kayaalp Seon-Hahn Kim Jim S.Khan Li-Jen Kuo Atsushi Nishimura Fernanda Nogueira Junji Okuda Avanish Saklani Ali A.Shafik Ming-Yin Shen Jung-Tack Son Jun-Min Song Dong-Hui Sun Keisuke Uehara Gui-Yu Wang Ye Wei Zhi-Guo Xiong Hong-Liang Yao Gang Yu Shao-Jun Yu Hai-Tao Zhou Suk-Hwan Lee Petr V.Tsarkov Chuan-Gang Fu Xi-Shan Wang The International Alliance of NOSES | 2019 | Gastroenterology Report2019，7，1： | 3 |
| 12 | A MODEL FOR PREDICTING PHASE INVERSION IN OIL-WATER TWO-PHASE PIPE FLOW显示文摘Experiments of phase inversion characteristics for horizontal oil-water two-phase flow in a stainless steel pipe loop (25.7 mm inner diameter,52 m long) are conducted. A new viewpoint is brought forward about the process of phase inversion in oil-water two-phase pipe flow. Using the relations between the total free energies of the pre-inversion and post-inversion dispersions, a model for predicting phase inversion in oil-water two-phase pipe flow has been developed that considers the characteristics of pipe flow. This model is compared against other models with relevant data of phase inversion in oil-water two-phase pipe flow. Results indicate that this model is better than other models in terms of calculation precision and applicability. The model is useful for guiding the design for optimal performance and safety in the operation of oil-water two-phase pipe flow in oil fields. | GONG Jing LI Qing-ping YAO Hai-yuan YU Da | 2006 | Journal of Hydrodynamics2006，18，3： | 3 |
| 13 | Human podocyte injury in the early course of hypertensive renal injury显示文摘BACKGROUND Hypertension is prevalent in the general population and is regarded as the second leading cause of renal damage and dysfunction,outnumbered only by diabetes.However,the mechanisms remain unclear.AIM To investigate podocyte injury induced by hypertension in the early course without massive proteinuria or renal dysfunction.METHODS The hypertension group comprised 18 patients with hypertension accompanied by microalbuminuria,diagnosed with hypertensive renal injury according to biopsy results.For a comparison of pathological changes in renal tissue,control group 1 comprised 10 healthy volunteers,and control group 2 comprised 16 patients who underwent surgery for renal trauma.RESULTS The hypertension group had significantly higher blood pressure(P=0.000)and microalbuminuria(P=0.000)compared with control group 1.In the hypertension group,urinary podocytes were detected following positive staining of podocytespecific nephrin and/or CD2-associated protein(CD2AP)in urine sediment.Podocyte foot process fusion and a significant decrease in nephrin and/or CD2AP expression in glomeruli were observed in the hypertension group compared with control group 2.This indicated that hypertension caused podocyte injury and detachment from the glomerular basement membrane,which was consistent with urinary detection of podocytes.CONCLUSION Our results suggest that podocyturia appears early in the course of hypertensive renal injury,and may be a sensitive marker for early prediction of hypertensive renal injury. | Da Sun Jiao-Jiao Wang Wei Wang Juan Wang Li-Ning Wang Li Yao Ying-Hui Sun Zi-Long Li | 2019 | World Journal of Clinical Cases2019，7，22： | 3 |
| 14 | Transcription of the putative tumor suppressor gene HCCS1 requires binding of ETS-2 to its consensus near the transcription start site显示文摘hepatocellular 癌压制或 1 (HCCS1 ) 基因被在肝癌症的从杂合现象(17p13.3 ) 的损失的一个占优势的区域的两位置的克隆并且由为在大规模 transfection 试金影响房间增长的基因的功能的屏蔽识别。它的过去在在房间文化的房间增长和在裸体鼠标的肿瘤生长的抑制的表达式结果。理解它的抄写规定,倡导者建筑学详细被把了。抄写的主要开始被对 C 残余的教材扩展印射, ATG 在上游的 177 核苷酸上哄骗。由在短暂 transfection 试金估计最小的倡导者(到 +148 区域的 -60 )的一套扫描连接器的异种的倡导者活动,我们发现 +1 到 40 区域是的+对 HCCS1 基因抄写批评,为抄写因素 NF-kappaB (到 +7 的 -21 和到 +26 的 +40 )的包含的有约束力的地点, p53 (到 +9 的 +29 )和 et (到 +20 的 +4 和到 +39 的 +23 )。生物化学、分子的分析表明 ETS 抄写因素 ETS-2 和 Elf-1 在 situ 绑在二个 et 地点并且显著地作出贡献到抄写结盟 HCCS1 基因的活跃状态,当时 NF-kappaB , p53 和 ETS 家庭( ETS-1 和 NERF2 )的二个另外的成员看起来起很少作用。我们的观察提供卓见进 HCCS1 抄写规定的机械学的方面。房间研究(2006 ) 16:780-796。做 i:10.1038/sj .cr.7310092;出版联机 2006 年 9 月 5 日。 | Jing De Zhu Qi Fei Peng Wang Fei Lan Da Qin Mao Hong Yu Zhang Xue Biao Yao | 2006 | Cell Research2006，16，9： | 3 |
| 15 | Cardiac differentiation and electrophysiology characteristics of bone marrow mesenchymal stem cells显示文摘 | LIU Bo-wu LU An-lin HOU Jing HUANG Wei LI Yao HOU Zhao-lei HOU Hong DA Jing YANG Na Al Shi-yi | 2012 | Chinese Medical Journal2012，，18： | 3 |
| 16 | AM ultrasound-modulated optical tomography with real-time FFT显示文摘We report on a novel ultrasound-modulated optical tomographic technique applied in dense tissue-simulating turbid media. A 1 MHz ultrasound beam is focused into the media to modulate and tag the scattering laser light passing through the ultrasonic focused zone. A 10 kHz sinusoidal wave, as a detection wave, is carried by the ultrasound beam through amplitude-modulation (AM). The scattering photons that come from the focused zone carry the modulated information, received by detector and demodulated by real-time fast Fourier transform (FFT). The optical tomographic images of the tissue simulating media and buried object are reconstructed with AM spectral intensity. This method can be applied to imaging three-dimensional tissue structures with different optical parameters. For the first time, we obtained the tomography of buried objects in biological tissue phantoms with a detecting depth of 30 mm. | Yong Yao Da Xing Yonghong He | 2001 | Chinese Science Bulletin2001，46，22： | 3 |
| 17 | Quantitative Analysis of Eight Ginsenosides in Red Ginseng Using Ginsenoside Rg1 as Single Reference Standard显示文摘Objective:To develop a reversed-phase high-performance liquid chromatography method for the quantification of major ginsenosides in red ginseng(RG,the steamed and dried root of the cultivar of Panax ginseng C.A.Mey).Methods:A feasible method was developed in strict accordance with chromatographic properties of eight ginsenosides.Their contents could be unveiled with conventional external standard method,or as an alternative,using ginsenoside Rg1 as the single reference standard by means of seven conversion factors.Those parameters had been validated on different chromatographic columns and instruments.Results:Twenty-one batches of RG samples were determined.In addition,the chromatograms of RG and confusing species,including Panax ginseng,Panax quinquefolium,and Panax notoginseng,were apparently different.Conclusions:The method was proved to be efficient for the quality control of RG. | Juan Da Qiu-Rong Wang Yan Wang Shuai Yao Yong Huang Wen-Long Wei Jian Liang Yao Shen Gerhard Franz De-An Guo | 2021 | World Journal of Traditional Chinese Medicine2021，7，1： | 2 |
| 18 | Ultrastructural observation of liver tissue ablation induced by high-intensity focused ultrasound显示文摘UltrastructuralobservationoflivertissueablationinducedbyhighintensityfocusedultrasoundCHENGShuQun1,ZHOUXinDa1,TANGZhaoYo... | CHENG Shu Qun 1, ZHOU Xin Da 1, TANG Zhao You 1, YU Yao 1, BAO Su Su 2 and QIAN De Chu 2 | 1997 | World Journal of Gastroenterology1997，3，3： | 2 |
| 19 | Infrared fluorescence imaging of infarcted hearts with A@S nanodots显示文摘Ag2S nano dots have already bee n dem onstrated as promisi ng n ear-i nfrared (NIR-II, 1.0-1.45 pm) emitting nan oprobes with low toxicity, high penetration and high resolution for in vivo imaging of, for example, tumors and vasculature. In this work, we have systematically investigated the pote ntial applicati on of function alized Ag2S nano dots for accurate imaging of damaged myocardium tissues after a myocardial in farction in duced by either partial or global ischemia. Ag2S nano dots surface-function alized with the an giote nsin II peptide (ATI I) have show n over 10-fold enhanced binding efficiency to damaged tissues than non-specifically (PEG) functionalized Ag2S nanodots due to their interaction with the upregulated angiotensin II receptor type I (AT1R). It is demonstrated how the NIR-II images generated by ATII-functionalized Ag2S nanodots contain valuable information about the location and extension of damaged tissue in the myocardium allowing for a proper identification of the occluded artery as well as an in direct evaluati on of the damage level. The pote ntial applicati on of Ag2S nano dots in the n ear future for in vivo imaging of myocardial infarction was also corroborated by performing proof of concept whole body imaging experiments. | Dirk H. Ortgies Angel Luis Garcia-Villalon Miriam Granado Sara Amor Emma Martin Rodriguez Harrisson DA Santos Jingke Yao Jorge Rubio-Retama Daniel Jaque | 2019 | Nano Research2019，12，4： | 2 |
| 20 | Development and Evaluation of a Distance Learning System Based on CSCW显示文摘This paper described a distance learning system, which allows Internet users to conduct a lesson in real time from any kinds attached computers. Participants can jointly view and edit relevant multimedia information distributed through Internet. Teachers and students can also simultaneously communicate by voice and text to discuss the problems. Teacher can broadcast streaming PowerPoint presentation in real time to network users. In addition to sliders, presenters can broadcast video and audio simultaneously to deliver a live multimedia show online, and store their presentations for on demand playback. Teachers distributed in different places can also use cooperative editing tool to edit and encode existing digital content. We discussed some important design principles of the system. Then, the system configuration and the results of evaluation are also presented. The system has proved to be applicable to the distance learning based on CSCW (Computer Support Cooperative Work) in Internet. | Yin Hao,Zhu Guang\|xi,Li Xiao\|long,Zhu Yao\|ting,He Da\|an Electronic Engineering Department,Huazhong University of Science and Technology , Wuhan 430074,China | 2001 | Wuhan University Journal of Natural Sciences2001，6，Z1： | 2 |
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