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| 1 | Rapid growth and sterility of growth hormone gene transgenic triploid carp显示文摘Triploid carp(100%)with 150(3n=150)chromosomes were obtained by crossing the females of improved tetraploid hybrids(♀, 4n=200)of red crucian carp(♀)×common carp(♂)with the males of diploid yellow river carp(♂,2n=100).The crosses yielded transgenic triploid carp(positive triploid fish,44.2%of the progeny)and non-transgenic triploid carp(negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season.Morphologically,both the positive and negative triploid fish were similar.They had a spindle-shaped,laterally compressed,steel grey body with two pairs of barbells.Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents.The positive and negative triploid fish were raised in the same pond for 2 years.The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish.The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish.Thus,we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny. | YU Fan XIAO Jun LIANG XiangYang LIU ShaoJun ZHOU GongJian LUO KaiKun LIU Yun HU Wei WANG YaPing ZHU ZuoYan | 2011 | Chinese Science Bulletin2011，56，16： | 11 |
| 2 | Evolution of surface microstructure of Cu-50Cr alloy treated by high current pulsed electron beam显示文摘A Cu-50Cr alloy was treated by the high current pulsed electron beam(HCPEB)at 20 and 30 ke V with pulse numbers ranging from 1 to 100.Surface morphologies and microstructures of specimens before and after the treatments were investigated by employing scanning electron microscopy and X-ray diffraction.Results show that the HCPEB technique is able to induce remarkable surface modifications for the Cu-50Cr alloy.Cracks in Cr phases appear even after one-pulse treatment and their density always increases with the pulse number.Formation reason for these cracks is attributed to quasi-static thermal stresses accumulated along the specimen surface.Craters with typical morphologies are formed due to the dynamic thermal field induced by the HCPEB and they are found to prefer the sites near cracks or boundaries between neighboring Cr phases.Another microstructural characteristic produced by the HCPEB is the fine Cr spheroids,which are determined to be due to occurrence of liquid phase separation in the Cu-50Cr alloy.Finally,a general microstructural evolution profile that incorporates various HCPEB-induced surface features is tentatively outlined. | CHAI LinJiang ZHOU ZhiMing XIAO ZhiPei TU Jian WANG YaPing HUANG WeiJiu | 2015 | Science China(Technological Sciences)2015，58，3： | 5 |
| 3 | Preliminary observation results of the Coherent Beacon Systemonboard the China Seismo-Electromagnetic Satellite-1显示文摘This paper reports, for the first time, observation results of the Coherent Beacon System(CBS) onboard the China SeismoElectromagnetic Satellite-1(CSES-1). We describe the CBS, and the Computerized Ionospheric Tomography(CIT) algorithm program is validated by numerical experiment. Two examples are shown, for daytime and nighttime respectively. The Equatorial Ionization Anomaly(EIA) can be seen, and the northern crest core is located at ~20°N in the reconstruction image at 07:28 UTC on 20 July 2018(daytime).Disturbances are shown in the reconstruction image at 18:40 UTC on 13 July 2018(nighttime). We find that beacon measurements are more consistent with ionosonde measurements than model results, by comparing Nm F2 at three sites at Lanzhou, Chongqing, and Kunming; consistency with ionosonde measurements validates beacon measurements. Finally, we have studied Vertical Total Electron Content(VTEC) variations from ground to ~500 km(the height of CSES-1 orbit) and ratios of VTEC between beacon measurements and CODE(Center for Orbit Determination in Europe) data. VTEC variation from ground to ~500 km has a range of 7.2–16.5 TECU for the daytime case and a range of 1.1–1.7 TECU for the nighttime case. The Beacon/CODE ratio of VTEC varies with latitude and time. The mean Beacon/CODE ratio is 0.69 for the daytime case and 0.26 for the nighttime case. The fact that the nighttime case yields lower ratios indicates the higher altitude of the ionosphere during nighttime when the ionosphere is assumed to be a thin layer. | Liang Chen Ming Ou YaPing Yuan Fang Sun Xiao Yu WeiMin Zhen | 2018 | Earth and Planetary Physics2018，2，6： | 3 |
| 4 | The Rice'NUTRITION RESPONSE AND ROOT GROWTH' (NRR) Gene Regulates Heading Date显示文摘Dear Editor, As one of the most important developmental traits,flowering time has been extensively investigated in the long-day plant Arabidopsis.Multiple factors are involved,including photoperiod and/or temperature (Amasino,2010).Flowering locus T (FT) is known to be an important integrator of different flowering pathways (Taoka et al.,2011).Genes of the CONSTANS (CO) family also play key roles in regulating flowering time.The CO protein consists of a zinc finger and a CCT domain shared by a group of plant-specific transcription factors that regulate photoperiodic flowering response and circadian rhythms in Arabidopsis.In the case of short-day plants such as rice,previous studies have also revealed a number of genes that control heading date,such as the CO ortholog Heading date 1 (Hdl) and,at its downstream,Heading date 3a (Hd3a),an ortholog of FT (Kojima et al.,2002;Komiya et al.,2008).Although the main photoperiodic flowering regulators are highly conserved between Arabidopsis and rice,there are genes that act differently relating to the daylength property of rice,such as Early heading datel (Ehdl),Indeterminate1 (OslD1),the MADS-box gene OsMADS51 (Doiet al.,2004;Kim et al.,2007;Park et al.,2008),and Ghd7 (Xue et al.,2008). | Yuman Zhang Ge Zhang Na Xiao Lina Wang Yaping Fu Zongxiu Sun Rongxiang Fang Xiaoying Chen | 2013 | Molecular Plant2013，6，2： | 3 |
| 5 | Synthesis of MoX2 (X =Se or S) monolayers with high-concentration 1T'phase on 4H/fcc-Au nanorods for hydrogen evolution显示文摘Controlled synthesis of transition metal dichalcogenide (TMD) monolayers with unusual crystal phases has attracted increasing attention due to their promising applications in electrocatalysis.However,the facile and large-scale preparation of TMD monolayers with high-concentration unusual crystal phase still remains a challenge.Herein,we report the synthesis of MoX2 (X =Se or S) monolayers with high-concentration semimetallic 1T'phase by using the 4H/face-centered cubic (fcc)-Au nanorod as template to form the 4H/fcc-Au@MoX2 nanocomposite.The concentrations of 1T'phase in the prepared MoSe2 and MoS2 monolayers are up to 86% and 81%,respectively.As a proof-of-concept application,the obtained Au@MoS2 nanocomposite is used for the electrocatalytic hydrogen evolution reaction (HER) in acid medium,exhibiting excellent performance with a low overpotential of 178 mV at the current density of 10 mNcm^2,a small Tafel slope of 43.3 mV/dec,and excellent HER stability.This work paves a way for direct synthesis of TMD monolayers with high-concentration of unusual crystal phase for the electrocatalytic application. | Zhengqing Liu Xiao Zhang Yue Gong Qipeng Lu Zhicheng Zhang Hongfei Cheng Qinglang Ma Junze Chen Meiting Zhao Bo Chen Ye Chen Xue-Jun Wu Pengfei Yin Lin Gu Yaping Du Hua Zhang | 2019 | Nano Research2019，12，6： | 3 |
| 6 | Surface engineering of titania nanotubes incorporated with double-layered extracellular vesicles to modulate inflammation and osteogenesis显示文摘Titania nanotubes(TNT)generated on titanium implant are emerged as important modification technique to facilitate bone regeneration.Mesenchymal stem cells(MSCs)-derived exosomes are membrane bound extracellular vesicles(EVs),which play an important role in tissue regeneration.The objective of this study was to generate an EVs hybrid TNT aiming at regulating inflammation,MSCs recruitment and osteogenesis.We isolated EVs from MSCs(MSCs EVs)and 3-day osteogenically differentiated MSCs(3d EVs).MSC EVs and 3d EVs exhibited round morphology under TEM,which also showed robust internalization by human bone marrow derived MSCs(hBMSCs).Next,we fabricated 3d EVs/MSC EVs hybrid TNT.When inflammatory macrophages were co-cultured with EVs hybrid TNT,the gene and protein expression of inflammatory cytokine were significantly reduced.Macrophage morphology was also examined by confocal laser scanning microscopy(CLSM)and scanning electron microscopy(SEM).Further migratory ability study using hBMSCs indicated significant enhancement of MSCs migration in EVs hybrid TNT.In addition,we further demonstrated significant increase of osteogenic differentiation of hBMSCs in EVs hybrid TNT.This study suggests that EVs hybrid TNT may serve as a viable therapeutic approach to enhance osteogenesis and bone regeneration. | Qingyu Zhao Yi Zhang Lan Xiao Haiping Lu Yaping Ma Qi Liu Xin Wang | 2021 | Regenerative Biomaterials2021，8，3： | 3 |
| 7 | DC-CIK联合化疗治疗非小细胞肺癌的临床疗效评价(英文)显示文摘Objective: The aim of the study was to evaluate the safety and therapeutic effects of autologous dendritic cells co-cultured with cytokine-induced killer cells (DC-CIK) combined with chemotherapy in advanced non-small cell lung cancer (NSCLC) patients. Methods: Fifty patients with advanced NSCLC (stages III to IV), who had received therapies in our Center (Department of Biotherapy, Affiliated to Cancer Hospital of Shanxi Medical University, Taiyuan, China) from August 2008 to January 2010, were treated by DC-CIK + chemotherapy as the combined treatment group; fifty advanced NSCLC patients treated with chemotherapy at the same time served as controls. The immunologic function, short-term therapeutic effects, the 1-year survival rate, the life quality, the chemotherapy side effects were compared between the two groups, the safety and therapeutic effects of DC-CIK cells therapy were observed too. Results: There was no obvious change of subsets of T cells in peripheral blood before and after therapy in DC-CIK + chemotherapy group, and IFN-γ was improved after therapy in this group (P < 0.05); in chemotherapy alone group, the ratios of CD3+CD4+, CD3+CD8+, CD3-CD56+ cells and the secretion of IL-2, TNF-α decreased significantly after therapy (P < 0.05); the ratios of CD3+CD8+, CD3+CD56+ were improved after cell culture (P < 0.05). The disease control rate (DCR) of DC-CIK + chemotherapy group was higher than that in the chemotherapy alone group (78.0% vs 56.0%, P < 0.05); the 1-year survival rates of DC-CIK + chemotherapy group and chemotherapy alone group were 50% and 44% respectively, had no significant difference. Compared with chemotherapy alone group, the occurrence of chemotherapy side effects (including bone marrow suppression, nausea and vomiting, peripheral nerve toxicity) was less in the DC-CIK + chemotherapy group (P < 0.05). The physical and appetite were better in DC-CIK + chemotherapy group after therapy. Conclusion: To compare with simple chemotherapy, DC-CIK + chemotherapy for advanced NSCLC is safe and effective, and it can improve patients' life quality and remission rate, and prolong their survival time. | Junping Zhang Guanghua Mao Yaping Han Xiaoling Yang Huijing Feng Linzi Jia Ting Zhi Yan Xiao Libin Zhang Jiangtao Wang Tianliang Shi | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，2： | 3 |
| 8 | Molecular Systematics of Xenocyprinae (Teleostei: Cyprinidae): Taxonomy, Biogeography, and Coevolution of a Special Group Restricted in East Asia显示文摘 | Wuhan Xiao Yaping Zhang Huanzhang Liu | 2001 | Molecular Phylogenetics and Evolution2001，，2： | 2 |
| 9 | Photocatalytic degradating methyl orange in water phase by UV-irradiated CdS carried by carbon nanotubes显示文摘A new candidate for photocatalytic degradating organic dyes,CdS carried by car-bon nanotubes(CdS/CNTs) ,is reported. The degradation ratio curves of methyl orange in water phase show that the capability for degradating organic molecules of CdS/CNTs is obviously higher than that of separated CdS. The degradation ca-pability enhances as the increase of the amount of net CdS catalyst,the ratio of carbon nanotubes to CdS,and the area of the template,and is influenced by the pH value and the temperature of aqueous solution. These results suggest that the photocatalyst of CdS/CNTs is very suitable for potential applications in organic waste removal from water. | LI ChenSha TANG YaPing KANG BoNan WANG BinSong ZHOU Feng MA Qiang XIAO Ji WANG DaZhi LIANG Ji | 2007 | Science China(Technological Sciences)2007，50，3： | 2 |
| 10 | Moleeular systemafics of Xenocyprinae(Telestei:Cyprinidae):taxonomy, biogeography, and eoevolution of a special group restricted in east Asia显示文摘 | Xiao Wuhan Zhang Yaping Liu Huanzhang | 2001 | Mol Phylogenet Evol2001，，18： | 1 |
| 11 | Molecular systematics of xenocyptinae ( Teleostei : Cyptinidae) : taxonomy biogeography and coevolution of a special group restricted in East Asia显示文摘 | Xiao Wuhan Zhang Yaping Liu Huanhang | 2001 | Molecuale Phylogentics and Evolutionm2001，18，2： | 1 |
| 12 | Synthesis of Few‐Layer MoS<sub>2</sub> Nanosheet‐Coated TiO<sub>2</sub> Nanobelt Heterostructures for Enhanced Photocatalytic Activities显示文摘 | Weijia Zhou Zongyou Yin Yaping Du Xiao Huang Zhiyuan Zeng Zhanxi Fan Hong Liu Jiyang Wang Hua Zhang | 2012 | Small2012，，1： | 1 |
| 13 | Light-controllable charge-reversal nanoparticles with polyinosinic-polycytidylic acid for enhancing immunotherapy of triple negative breast cancer显示文摘Nucleic acid drugs are highly applicable for cancer immunotherapy with promising therapeutic effects, while targeting delivery of these drugs to disease lesions remains challenging. Cationic polymeric nanoparticles have paved the way for efficient delivery of nucleic acid drugs, and achieved stimuli-responsive disassembly in tumor microenvironment(TME). However, TME is highly heterogeneous between individuals, and most nanocarriers lack active-control over the release of loaded nucleic acid drugs, which will definitely reduce the therapeutic efficacy. Herein, we have developed a lightcontrollable charge-reversal nanoparticle(LCCN) with controlled release of polyinosinic-polycytidylic acid [Poly(I:C)] to treat triple negative breast cancer(TNBC) by enhanced photodynamic immunotherapy. The nanoparticles keep suitably positive charge for stable loading of Poly(I:C), while rapidly reverse to negative charge after near-infrared light irradiation to release Poly(I:C). LCCN-Poly(I:C) nanoparticles trigger effective phototoxicity and immunogenic cell death on 4 T1 tumor cells, elevate antitumor immune responses and inhibit the growth of primary and abscopal 4 T1 tumors in mice. The approach provides a promising strategy for controlled release of various nucleic acid-based immune modulators, which may enhance the efficacy of photodynamic immunotherapy against TNBC. | Lei Fang Zitong Zhao Jue Wang Ping Xiao Xiangshi Sun Yaping Ding Pengcheng Zhang Dangge Wang Yaping Li | 2022 | Acta Pharmaceutica Sinica B2022，12，1： | 1 |
| 14 | Visualization of the electrocatalytic activity of three- dimensional MoSe2@reduced graphene oxide hybrid nanostructures for oxygen reduction reaction显示文摘nanostructured 转变金属 dichalcogenides (TMD ) 的开发材料作为氧的新奇 electrocatalyst 候选人,减小反应(ORR ) 是新策略支持非宝贵的金属 ORR 催化剂的开发。在这个工作,一三维(3D ) 像玫瑰花蕾的 MoSe 2 nanostructures 的混血儿在减少的 graphene 上支持了氧化物(rGO ) nanosheets 成功地通过灵巧的热水的策略被综合。混合 nanostructure 显示出的准备 MoSe 2@rGO 与纯 MoSe 2, rGO ,和他们的简单物理混合的相比在碱的媒介为 ORR 提高了 electrocatalytic 活动,它能得益于 ultrathin MoSe 2层的富有地暴露的活跃的边地点的优秀的氧吸附能力,传导性和 rGO 站台的限制聚集的效果,以及唯一的 3D 混合材料的像玫瑰花蕾的建筑学。向 ORR 的 MoSe 2@rGO 混血儿的 electrocatalytic 活动比得上商业 Pt/C 催化剂的。并且支持的反应被揭示包含一将近,由获得的 Koutecky-Levich 的分析的 four-electron-dominated ORR 过程阴谋。扫描电气化学的显微镜学( SECM )技术与在一个单个实验与高空间的分辨率和不同催化剂的同时评估的活动样品的本地催化活动调查的优点,进一步被使用调查 MoSe 2@rGO 的本地 ORR electrocatalytic 活动并且通过使用不同样品潜力另外的催化剂样品把它与那些作比较。优秀稳定性和对甲醇的 3D nanostructured MoSe 2@rGO 混血儿的甲醇忍耐进一步在燃料房间和金属空气电池在潜在的应用程序的碱的答案作为有希望的 ORR electrocatalyst 证明 3D nanostructured MoSe 2@rGO 混合。 | Shuli Xin Zhengqing Liu Li Ma Yao Sun Chunhui Xiao Fei Li Yaping Du | 2016 | Nano Research2016，9，12： | 1 |
| 15 | Novel Combination Therapy for Triple-Negative Breast Cancer based on an Intelligent Hollow Carbon Sphere显示文摘Triple-negative breast cancer(TNBC)is a subtype of breast cancer with high mortality,and the efficacy of monotherapy for TNBC is still disappointing.Here,we developed a novel combination therapy for TNBC based on a multifunctional nanohollow carbon sphere.This intelligent material contains a superadsorbed silicon dioxide sphere,sufficient loading space,a nanoscale hole on its surface,a robust shell,and an outer bilayer,and it could load both programmed cell death protein 1/programmed cell death ligand 1(PD-1/PD-L1)small-molecule immune checkpoints and small-molecule photosensitizers with excellent loading contents,protect these small molecules during the systemic circulation,and achieve accumulation of them in tumor sites after systemic administration followed by the application of laser irradiation,thereby realizing dual attack of photodynamic therapy and immunotherapy on tumors.Importantly,we integrated the fasting-mimicking diet condition that can further enhance the cellular uptake efficiency of nanoparticles in tumor cells and amplify the immune responses,further enhancing the therapeutic effect.Thus,a novel combination therapy“PD-1/PD-L1 immune checkpoint blockade+photodynamic therapy+fasting-mimicking diet”was developed with the aid of our materials,which eventually achieved a marked therapeutic effect in 4T1-tumor-bearing mice.The concept can also be applied to the clinical treatment of human TNBC with guiding significance in the future. | Yue Yin Yaping Yan Biao Fan Wenping Huang Jie Zhang Hai-Yan Hu Xiaoqiong Li Dongbin Xiong Shu-Lei Chou Yao Xiao Hai Wang | 2023 | Research2023，，4： | 1 |
| 16 | Polyploidization and epigenetics显示文摘The occurrence of polyploidy,or whole genome duplication,can result in instantaneous speciation.Because new polyploids are immediately reproductively isolated from their non-polyploid congeners,polyploidization has played an important role in the diversification of flowering plants and some vertebrates.Newly formed polyploids must respond to this instantaneous genomic change,which resembles 'genome shock' syndrome to survive and reproduce successfully.Epigenetic changes,which do not cause changes to the sequence of DNA,can significantly contribute to the survival of and ultimately to the evolutionary success of new polyploids.Epigenetic regulation,both transcriptional and post-transcriptional,entails changes in DNA methylation,gene status and/or nucleolus dominance.These changes provide effective and flexible ways for a new polyploid to respond quickly to the enormous change in genetic material,to survive and potentially reproduce.We examine and assess certain epigenetic phenomena and possible pathways that may facilitate the evolutionary success of polyploid organisms. | LI ZhiHui LU Xiang GAO Yun LIU ShaoJun TAO Min XIAO Heng QIAO YunQian ZHANG YaPing LUO Jing | 2011 | Chinese Science Bulletin2011，56，3： | 1 |
| 17 | Molec- ular systematics of Xenocyprinae: taxonomy, biogeog- raphy,and coevolution of a special group restricted in east Asia显示文摘 | Xiao WuHan Zhang YaPing Liu HuanZhang | 2001 | Mol PhylogenetEvol2001，18，4： | 1 |
| 18 | Mo- lecular systematics of Xenocyprinae ( Telestei: Cyprini- dae): taxonomy, biogeography, and coevolution of a special group restricted in east Asia显示文摘 | XIAO Wuhan ZHANG Yaping LIU Huanzhang | 2001 | Mol Phylogenet Evol2001，18，： | 1 |
| 19 | Novel mutation c.1210-3C>G in cis with a poly-T tract of 5T affects CFTR mRNA splicing in a Chinese patient with cystic fibrosis显示文摘Cystic fibrosis(CF)is a rare autosomal recessive disease with only one pathogenic gene cystic fibrosis transmembrane conductance regulator(CFTR).To identify the potential pathogenic mutations in a Chinese patient with CF,we conducted Sanger sequencing on the genomic DNA of the patient and his parents and detected all 27 coding exons of CFTR and their flanking intronic regions.The patient is a compound heterozygote of c.2909G>A,p.Gly970Asp in exon 18 and c.1210-3C>G in cis with a poly-T of 5T(T5)sequence,3 bp upstream in intron 9.The splicing effect of c.1210-3C>G was verified via minigene assay in vitro,indicating that wild-type plasmid containing c.1210-3C together with T7 sequence produced a normal transcript and partial exon 10-skipping-transcript,whereas mutant plasmid containing c.1210-3G in cis with T5 sequence caused almost all mRNA to skip exon 10.Overall,c.1210-3C>G,the newly identified pathogenic mutation in our patient,in combination with T5 sequence in cis,affects the CFTR gene splicing and produces nearly no normal transcript in vitro.Moreover,this patient carries a p.Gly970Asp mutation,thus confirming the high-frequency of this mutation in Chinese patients with CF. | Xinyue Zhao Keqiang Liu Wenshuai Xu Meng Xiao Qianli Zhang Jiaxing Song Keqi Chen Yaping Liu Xinlun Tian Kai-Feng Xu Xue Zhang | 2022 | Frontiers of Medicine2022，16，1： | 1 |
| 20 | Selective catalytic reduction of NOx with NH3 over V2O5-WO3/TiO2-ZrO2 monolith catalysts显示文摘 | LI Peng ZHANG Yaping XIAO Rui | 2013 | Journal of Central South University (Science and Technology)2013，44，4： | 1 |
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