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| 1 | Identification and Functional Characterization of a NovelΔ12 Fatty Acid Desaturase Gene from Haematococcus pluvialis显示文摘The freshwater microalga Haematococcus pluvialis accumulates large amounts of fatty acids in response to adverse conditions.However,the key fatty acid desaturase genes in H.pluvialis remain unknown.In this study,we cloned and functionally characterized aΔ12 fatty acid desaturase gene,and designated it as HpFAD2.The open reading frame of HpFAD2 consisted of 1137 base pairs and encoded 378 amino acids.The deduced polypeptide showed 70%identity to other endoplasmic reticulumΔ12 fatty acid desaturases,whereas it had only 44%identity to plastidΔ12 fatty acid desaturases.The PSORT algorithm and phylogenetic analysis further confirmed its affiliation to the endoplasmic reticulumΔ12 fatty acid desaturases.Heterologous expression was performed in Saccharomyces cerevisiae cells transformed with the recombinant plasmid pYES2-HpFAD2.Two additional fatty acids(C16:2 and C18:2)were detected in the yeast transformants.The results indicatedΔ12 desaturation activity and substrate preference for C18:1 over C16:1.The transcriptional levels of H.pluvialis HpFAD2 at different growth stages were measured by quantitative polymerase chain reaction(PCR),indicating that the HpFAD2 transcriptional levels were significantly higher in red cells than those in green cells.Our study brings more insight into the fatty acid biosynthetic pathway of H.pluvialis. | ZHANG Lin CHEN Wenbi YANG Shuping ZHANG Yuanbo XU Jilin YANG Dongjie WU Zuyao LIU Tong CAO Jiayi | 2020 | Journal of Ocean University of China2020，19，6： | 2 |
| 2 | Determination of erythrocyte transit time through micropores 显示文摘 | Koutsouris D Guillet R Wenby RB | 1989 | Biorheology1989，26，： | 1 |
| 3 | Effects of nitric oxide on red blood cell deformability 显示文摘 | Bor-Kucukatay M Wenby RB Meiselman H J | 2003 | Am J Physiol Heart Circ Physiol2003，284，5： | 1 |
| 4 | Determination of particle sedimentation rate by ultrasonic interferometry: role of particle size, density and volume fraction 显示文摘 | Razavian S M Wenby R B Fisher T C | 1997 | Biorheology1997，34，45： | 1 |
| 5 | Abnormal blood theology and chronic low grade inflammation : possible risk factors for accelerated atherosclerosis and cor- onary artery disease in Lewis negative subjects 显示文摘 | AlexyT Pais E Wenby RB | 2015 | Atheroselerosis2015，239，1： | 1 |
| 6 | Amine modified graphene as reversed-dispersive solid phase extraction materials com- bined with liquid chromatography tandem mass spectrometry for pesti- cide multi-residue analysis in oil crops显示文摘 | Guan Wenbi Li Zhuonan Zhang Hongyan | 2013 | Journal of Chromatogra- phy A2013，1286，8： | 1 |
| 7 | Perceived learning needs of patients with heart failure显示文摘 | Wenby D Phylli S | 1999 | heartlung1999，28，： | 1 |
| 8 | Influence of cellular properties on red cell aggregation 显示文摘 | Nash G B Wenby R B Sowemimo Coker S O | 1987 | Clin Hemorheol Microcirc1987，7，5： | 1 |
| 9 | Effects of nitric oxide on red blood cell deformability显示文摘 | Eor-Kucukatay M Wenby RB Meiselman HJ | 2003 | J Am Physiol Heart Circ Physiol2003，284，5： | 1 |
| 10 | Effects of dextran molecular weight on red blood cell aggregation显示文摘 | Neu Bjoern Wenby Rosalinda Meiselman Herbert J | 2008 | Biophysical Journal2008，95，6： | 1 |
| 11 | Rheological tests for monitoring disease activity determination of specific red blood cell aggregation indices via an automated system 显示文摘 | Bauersachs R M Wenby R B | 1989 | Clinical Hemorheology1989，9，1： | 1 |
| 12 | Amine modified graphene as reversed-dispersive solid phase extraction materials combined with liquid chromatography-tandem mass spectrometry for pesticide multi-residue analysis in oil crops显示文摘 | Wenbi Guan Zhuonan Li Hongyan Zhang Huijie Hong Natalie Rebeyev Yong Ye Yongqiang Ma | 2013 | Journal of Chromatography A2013，，： | 1 |
| 13 | Determination and study on dissipation of I-naphthylacetir acid in garlic and soil using high per formance liquid chromatography-tandem mass speclrometry显示文摘 | GUAN Wenbi XU Pengjun WANG Kai | 2011 | Food and Chemical Toxicology2011，49，2011： | 1 |
| 14 | Autographa Californica Multiple Nucleopolyhedrovirus P48(Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles显示文摘Our previous study has shown that the Autographa californica multiple nudeopolyhedrovirus(AcMNPV)p48(acl03)gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions(ODVs).However,the exact role of p48 in the morphogenesis of ODVs remains unknown.In this study,we demonstrated that p48 was required for the efficient formation of intranuclear microvesicles.To further understand its functional role in intranuclear microvesicle formation,we characterized the distribution of the P48 protein,which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and ODVs.In AcMNPV-infected cells,P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in ODVs,with a limited but discernible distribution in the plasma membrane,nuclear envelope,intranuclear microvesicles,and ODV envelope.Furthermore,coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation,F48 associated with Ac93 in the absence of viral infection. | Yan Wang Qingyun Cai Jiannan Chen Zhihong Huang Wenbi Wu Meijin Yuan Kai Yang | 2019 | Virologica Sinica2019，34，6： | 1 |
| 15 | Hemorheological abnormalities in stable angina and acute coronary syndromes显示文摘 | F. Jung S. Forconi N. Antonova Byoung K. Lee Azhil Durairaj Anilkumar Mehra Rosalinda B. Wenby Herbert J. Meiselman Tamas Alexy | 2008 | 2008 (1–4)2008，，1： | 1 |
| 16 | Effects of nitric oxide on red blood cell deformability 显示文摘 | Bor-Kucukatay M Wenby RB Melselman HJ | 2003 | Am J Physiol Heart Cire Physio12003，294，5： | 1 |
| 17 | Determination and Study on Residue and Dissipation of Florasulam in Wheat and SoilUnder Field Conditions显示文摘 | Zhuonan Li Wenbi Guan Huijie Hong | 2013 | Bull Environ Contam Toxicol2013，90，： | 1 |
| 18 | Effects of nitric oxide on red blood cell deformability 显示文摘 | Bor-Kucukatay M Wenby RB Meiselman HJ | 2003 | J Am Physlol Heart Circ Physiol2003，284，5： | 1 |
| 19 | He- morheological abnormalities in stable angina and acute coronary syndromes显示文摘 | Lee B K Durairaj A Mehra A Wenby R B | 2008 | Clin Hemorheol Microcirc2008，39，14： | 1 |
| 20 | Effects of nitric oxide on red blood cell deformability显示文摘 | Kucukatay M Wenby RB Meiselman HJ | 2003 | Am J Physiol Heart Cite Physiol2003，284，5： | 1 |
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