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| 1 | A review of composition evolution in Ni-based single crystal superalloys显示文摘Due to the outstanding creep performance, nickel-based single crystal superalloys(Ni-SXs) are extensively applied in modern aero-engine and industrial gas turbine. Apart from the special single crystal structure which is disadvantageous to extension of creep cracks, Ni-SXs derive the creep strength from intrinsic two-phase microstructure(γ phase and γ’ phase). Main microstructural parameters including volume fraction of γ’ phase and the lattice misfit, and the formation and distribution of precipitated phase are determined by the compositions of alloys. Besides, the creep properties are greatly influenced by these microstructural parameters and precipitated phase. This review has summarized the relationships between different alloying elements and microstructures and indicated their influence on creep properties of Ni-SXs. In addition, with the improvements of experimental methods and characterization technique, some recent discoveries have provided additional evidence to support or challenge the pervious creep theories of superalloys. In view of these new discoveries, this review has provided some perspectives which can be referenced in future compositional design of Ni-SXs. | Wanshun Xia Xinbao Zhao Liang Yue Ze Zhang | 2020 | Journal of Materials Science & Technology2020，44，9： | 16 |
| 2 | Establishment of a novel corneal endothelial cell line from domestic rabbit,Oryctolagus curiculus显示文摘To develop a rabbit corneal endothelial (RCE) cell line, in vitro culture of RCE cells was initiated from Oryctolagus curiculus corneas and a novel RCE cell line was established in this study. To initiate the primary culture of RCE cells, corneas from rabbit eyes were sliced and attached into glutin-coated wells with endothelial cell surface down. After being cultured at a time-gradient interval from 48 to 6 h, the corneal slices were detached and reattached into new wells, respectively. Cells in the wells containing only a pure population of RCE cells were collected and cultured in 20% FBS-DMEM/F12 medium con- taining chondroitin sulfate, ocular extract, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), carboxymethyl-chitosan, N-acetylglucosamine hydrochloride, glucosamine hydrochloride, culture medium of rabbit corneal stromal cells and oxidation-degradation products of chondroitin sul- fate at 37℃, 5% CO2. The cultured RCE cells, in quadrangle and polygonal shapes, proliferated to con- fluence 3 weeks later. During the subsequent subculture, the shape of RCE cells changed gradually from polygonal to more fibroblastic. A novel RCE cell line, growing at a steady rate, with a population doubling time of 53.8 h, has been established and subcultured to passage 67. Chromosome analysis showed that the RCE cells exhibited chromosomal aneuploidy with the modal chromosome number of 44. The results of immuno-cytochemical staining with neuron specific enolase (NSE) confirmed that the RCE cells were in neuroectodermal origin. Combined with the results of vascular endothelial growth factor (VEGF) treatment and endothelial cell morphology recovery, it can be concluded that the cell line established here is an RCE cell line. This RCE cell line may serve as a useful tool in theoretical re- searches of mammalian corneal endothelial cells, and may also have potential application in artificial corneal endothelium development. | FAN TingJun, ZHAO Jun, FU YongFeng, CONG RiShan, GUO RuiChao, LIU WanShun, HAN BaoQin, YU QiuTao & WANG JingCollege of Marine Life Sciences, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China | 2007 | Science China(Life Sciences)2007，50，2： | 15 |
| 3 | QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples显示文摘Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data'. | Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu Xiaoduo Lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li | 2019 | Molecular Plant2019，12，3： | 13 |
| 4 | Efficient elimination of multidrug-resistant bacteria using copper sulfide nanozymes anchored to graphene oxide nanosheets显示文摘Antibacterial nanomaterials have attracted growing interest for bacterial infection therapy.However,most nanomaterials eliminate bacteria either physically or chemically,which hampers their efficacy when dealing with multidrug-resistant bacteria.To overcome this,we integrated copper sulfide(CuS)nanoparticles with active graphene oxide nanosheets(GO NSs)to synthesize a superior nanocomposite(CuS/GO NC)that acts both physically and chemically on the bacteria.CuS/GO NC was produced using a facile hydrothermal method,whereby the CuS nanoparticles grew and were uniformly dispersed on the GO NSs in situ.We found that the CuS/GO NC possesses a unique needle-like morphology that physically damages the bacterial cell membrane.CuS/GO NC also exhibits high oxidase-and peroxidase-like activity,ensuring efficient generation of the reactive oxygen species•OH from H2O2,which kills bacteria chemically.These features endow the CuS/GO NC with excellent antibacterial capabilities to kill multidrug-resistant bacteria such as methicillin-resistant Staphylococcus aureus(MRSA)with only a single dose.Additionally,it was found that the CuS/GO NC accelerated the healing of infected wounds in vivo owing to its good biocompatibility as well as facilitation of cell migration and collagen secretion.This study provides a new strategy to combine the physical and chemical antibacterial modes of nanomaterials to develop more effective therapies to combat multidrug-resistant bacterial infections. | Wanshun Wang Binglin Li Huili Yang Zefeng Lin Lingling Chen Zhan Li Jiayuan Ge Tao Zhang Hong Xia Lihua Li Yao Lu | 2020 | Nano Research2020，13，8： | 8 |
| 5 | Effects of Carboxymethyl-Chitosan on Wound Healing in vivo and in vitro显示文摘Effects of carboxymethyl-chitosan with different molecular weights on wound healing were investigated in vivo and in vitro.A second degree burn model was performed in rats and the accelerative effects of carboxymethyl-chitosan on wound repair were observed.Contents of transforming growth factor(TGF)-β1,interleukin(IL)-6 and matrix metalloproteinase(MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay(ELISA).In vitro study evaluated the influence of carboxy-methyl-chitosan on cytokines secretion of fibroblasts and macrophages.In vivo results showed that carboxymethyl-chitosan effec-tively accelerated the wound healing process in burned rats(P<0.05).Levels of TGF-β1,IL-6 and MMP-1 in carboxymethyl-chitosan groups were significantly elevated,compared with control group(P<0.05).In vitro results indicated that carboxymethyl-chitosan significantly promoted the proliferation of fibroblasts and stimulated its IL-8 and IL-10 secretion at different incubation time,but it did not affect collagen secretion of fibroblasts.Carboxymethyl-chitosan enhanced phagocytosis ability of macrophages,and in-creased its tumor necrosis factor(TNF)-α secretion.In conclusion,carboxymethyl-chitosan promoted wound healing by activating macrophages,accelerating fibroblasts growth and exerting considerable effects on the secretion of a series of cytokines. | PENG Sikai LIU Wanshun HAN Baoqin CHANG Jing LI Minyu ZHI Xuan | 2011 | Journal of Ocean University of China2011，10，4： | 7 |
| 6 | Analgesis and Wound Healing Effect of Chitosan and Carboxymethyl Chitosan on Scalded Rats显示文摘Analgesis and wound healing effect of chitosan and carboxymethyl chitosan on scalded rats were investigated. A II degree scald model was established in rats, which was subsequently treated with chitosan and carboxymethyl chitosan solution, respectively. The concentration of bradykinin and 5-hydroxytryptophan was detected by assaying enzyme-linked immunosorbent. Healing condition was observed and pathological sections were made to determine the healing effect of chitosan and carboxymethyl chitosan. Results showed that the concentration of bradykinin and 5-hydroxytryptophan peaked at the third hour post-wound in all groups, while the concentration of hydroxyproline peaked at the seventh day post-wound in both chitosan and carboxymethyl chitosan group. The concentration of bradykinin and 5-hydroxytryptophan of carboxymethyl chitosan group was significantly lower than that of control(P < 0.05), while that of chitosan group was similar to that of control(P > 0.05). These findings indicated that carboxymethyl chitosan reduced the concentration of algogenic substances, resulting in analgesia. During the whole recovery process, the hydroxyproline concentration in chitosan and carboxymethyl chitosan group on day 3 and 7 was significantly higher than that of control(P < 0.01); however the significance of such a highness decreased on day 14(P < 0.05). These findings indicated that chitosan and carboxymethyl chitosan accelerated tissue repair. Meanwhile, chitosan performed better in healing than carboxymethyl chitosan in both decrustation and healing time. In conclusion, carboxymethyl chitosan showed significant analgesis and wound-healing promotion effect, but chitosan only showed wound-healing promotion effect. | HUANG Shuya HAN Baoqin SHAO Kai YU Miao LIU Wanshun | 2014 | Journal of Ocean University of China2014，13，5： | 5 |
| 7 | Pharmacokinetics and Biodegradation Performance of a Hydroxypropyl Chitosan Derivative显示文摘Hydroxypropyl chitosan(HP-chitosan) has been shown to have promising applications in a wide range of areas due to its biocompatibility, biodegradability and various biological activities, especially in the biomedical and pharmaceutical fields. However, it is not yet known about its pharmacokinetics and biodegradation performance, which are crucial for its clinical applications. In order to lay a foundation for its further applications and exploitations, here we carried out fluorescence intensity and GPC analyses to determine the pharmacokinetics mode of fluorescein isothiocyanate-labeled HP-chitosan(FITC-HP-chitosan) and its biodegradability. The results showed that after intraperitoneal administration at a dose of 10 mg per rat, FITC-HP-chitosan could be absorbed rapidly and distributed to liver, kidney and spleen through blood. It was indicated that FITC-HP-chitosan could be utilized effectively, and 88.47% of the FITC-HP-chitosan could be excreted by urine within 11 days with a molecular weight less than 10 k Da. Moreover, our data indicated that there was an obvious degradation process occurred in liver(< 10 k Da at 24 h). In summary, HP-chitosan has excellent bioavailability and biodegradability, suggesting the potential applications of hydroxypropyl-modified chitosan as materials in drug delivery, tissue engineering and biomedical area. | SHAO Kai HAN Baoqin DONG Wen SONG Fulai LIU Weizhi LIU Wanshun | 2015 | Journal of Ocean University of China2015，14，5： | 5 |
| 8 | Activities of the Deriviatives of Chitin on the Osbeoblast Proliferation and the Effect on Bone Strength in Ovariectomized Rats显示文摘This study intends to examine the effects of different concentrations of four kinds of degradations of chitin: glucosamine (GLC),N-acetyl-D-glucosamine (NAG), chitooligosaccharide(COS),CM-chitooligosaccharide (CM-COS) —on the proliferation of MC3T3-E1 cell line cultured in vitro. Results suggest that all of the glucoses mentioned above promoted the proliferation of osteoblast, and various concentrations have different effects: the proliferation was remarkable when the concentration of GLC, NAG, COS, CM-COS was 100 , 100 , 500 , 500 μg/mL ,respectively. Furthermore we choose the glucosamine as the material and study the effect on the bone strength in ovariectomized rats. The results showed that the middle does of glucosamine can significantly increase the bone strength of femur in ovariectomized rats. | WEI Changzheng LIU Wanshun HAN Baoqin DUAN Yixiang | 2006 | Wuhan University Journal of Natural Sciences2006，11，3： | 5 |
| 9 | Synthesis and Characterization of a Hydroxyethyl Derivative of Chitosan and Evaluation of Its Biosafety显示文摘Hydroxyethyl chitosan(HE-chitosan) is a water-soluble derivative of chitosan with many apparent biological properties. For example, it is non-toxic and rapidly biodegradable. Moreover, HE-chitosan has advantages in water-solubility, moisture retention and gelling property due to its hydroxyethyl group. However, the biocompatibility and biodegradability of this multifunctional derivative have rarely been documented although they are critical for its application in biomedical and clinical treatments. The purpose of this work was to evaluate the biosafety of HE-chitosan, and draw important clues for its diverse applications. HE-chitosan was synthesized and characterized its chemical structure with FTIR. Its molecular weight(MW) was determined by gel permeation chromatography(GPC), and its deacetylation degree(DD) was investigated through potentiometric analysis. The cytotoxicity of HE-chitosan on mouse fibroblast cell L929 was tested. The biocompatibility and biodegradability of HE-chitosan in rat and rabbit were evaluated. The FTIR results indicated that the hydroxyethyl groups were linked to C6 of chitosan. The GPC analysis confirmed that its Mw was about 90.01 k Da. It was also demonstrated that HE-chitosan had excellent biocompatibility and biodegradability in vivo and had no cytotoxicity on L929. These findings indicated that HE-chitosan can potentially be applied as a biomaterial in tissue engineering, drug delivery, and other biomedical fields. | SHAO Kai HAN Baoqin GAO Jinning SONG Fulai YANG Yan LIU Wanshun | 2015 | Journal of Ocean University of China2015，14，4： | 5 |
| 10 | A New Treatment of Depression for Drainage Network Extraction Based on DEM显示文摘Depressions in landscapes function as buffers for water and sediment. A landscape with depressions has less runoff,less erosion and more sedimentation than that without depressions. Sinks in digital elevation models (DEMs) can be considered the real features that represent depressions in actual landscapes or spurious features that result from errors in DEM creation. In many hydrological and erosion models,all sinks are considered as spurious features and,as a result,these models do not deal with the sinks that represent real depressions. Consequently,the surface runoff and erosion are overestimated due to removing the depressions. Aiming at this problem,this paper presents a new method,which deal with the sinks that represent real depressions. The drainage network is extracted without changing the original DEM. The method includes four steps:detecting pits,detecting depressions,merging depressions,and extracting drainage network. Because the elevations of grid cells are not changed,the method can also avoid producing new flat areas,which are always produced by the conventional filling methods. The proposed method was applied to the Xihanshui River basin,the upper reach of the Jialingjiang River basin,China,to automatically extract the drainage network based on DEM. The extracted drainage network agrees well with the reality and can be used for further hydrologic analysis and erosion estimation. | WANG Yan PENG Hong CUI Peng ZHANG Wanshun QIAO Fei CHEN Cai'er | 2009 | Journal of Mountain Science2009，6，4： | 3 |
| 11 | The Evaluation on Biological Properties of Carboxymethyl-chitosan and Carboxymethyl-chitin显示文摘Carboxymethyl-chitosan and carboxymethyl-chitin were prepared with the methods developed in our laboratory. The DS (degree of substitution) and DD (degree of deacetylation) of the carboxymethyl-chitosan were 103.14% and 97.18% respectively,while the DS of the carboxymethyl-chitin was 96.37%. Their effects on human fibroblasts,intradermal irritation test,in vitro and vivo degradability,and biocompatibility were evaluated. The results indicate that the polysaccharides at low concentrations can facilitate the growth of human fibroblasts and the carboxymethyl-chitosan at 100 μg mL-1 is the most effective. The polysaccharides at higher concentrations,however,inhibit the growth of fibroblasts. The PII (Primary Irritation Index) values of CM-chitosan and CM-chitin are both 0.0,which shows that they have no irritation reaction. Both of the polysaccharides show good degradability and biocompati-bility. Carboxymethyl-chitin degrades faster in vitro than carboxymethyl-chitosan. The latter,however,has no inflammatory reaction after being implanted in vivo for 7 d and shows better biocompatibility. This study may provide a scientific basis for the use of car-boxymethyl-chitosan and carboxymethyl-chitin as biomaterials. | CHANG Jing LIU Wanshun HAN Baoqin LIU Bing | 2008 | Journal of Ocean University of China2008，7，4： | 3 |
| 12 | Enzymatic properties of UFE,a novel marine fibrinolytic enzyme显示文摘A novel potent protease, Urechis unicinctus fibrinolytic enzyme (UFE), was firstly discovered. The enzymatic properties of UFE were further investigated. As a low molecular mass protein, UFE appeared to be very stable to heat and pH. When temperature was below 50 ℃, the remnant enzyme activity remained almost unchanged, but when temperature was raised to 60 ℃, the remnant enzyme activity began to decrease rapidly. UFE was quite stable in the range of pH value from 3 to 12, especially in slightly alkaline pH value. Mn2+, Cu2+ and Fe2+ ions were activators of UFE, while Fe3+ and Ag+ ions were inhibitors of UFE. Fe2+ ion along with Fe3+ ion might regulate UFE activity in vivo. The optimum pH and temperature of UFE were about 8 and 50 ℃, respectively. Other characteristics of this enzyme were also studied. Systematic research results are significant when UFE is applied for medical and industrial purposes. | WANG Dianliang LIU Wanshun HAN Baoqin | 2007 | Acta Oceanologica Sinica2007，26，2： | 3 |
| 13 | Pharmacokinetics and Biodegradation of Chitosan in Rats显示文摘Chitosan, an excellent biomedical material, has received a widespread in vivo application. In contrast, its metabolism and distribution once being implanted were less documented. In this study, the pharmacokinetics and biodegradation of fluorescein isothiocyanate(FITC) labeled and muscle implantation administrated chitosan in rats were investigated with fluorescence spectrophotometry, histological assay and gel chromatography. After implantation, chitosan was degraded gradually during its distribution to diverse organs. Among the tested organs, liver and kidney were found to be the first two highest in chitosan content, which was followed by heart, brain and spleen. Urinary excretion was believed to be the major pathway of chitosan elimination, yet 80% of chitosan administered to rats was not trackable in their urine. This indicated that the majority of chitosan was degraded in tissues. In average, the molecular weight of the degradation products of chitosan in diverse organs and urine was found to be <65 k Da. This further confirmed the in vivo degradation of chitosan. Our findings provided new evidences for the intensive and safe application of chitosan as a biomedical material. | LI Hui JIANG Zhiwen HAN Baoqin NIU Shuyi DONG Wen LIU Wanshun | 2015 | Journal of Ocean University of China2015，14，5： | 3 |
| 14 | Two-dimensional Numerical Model for Debris Flows in the Jiangjia Gully,Yunnan Province显示文摘Debris flows are recurrent natural hazards in many mountainous regions.This paper presents a numerical study on the propagation of debris flows in natural erodible open channels,in which the bed erosion and sedimentation processes are important.Based on the Bingham fluid theory,a mathematical model of the two-dimensional non-constant debris flow is developed.The governing equations include the continuity and momentum conservation equations of debris flow,the sediment convection-diffusion equation,the bed erosion-deposition equation and the bed-sediment size gradation adjustment equation.The yield stress and shear stress components are included to describe the dynamic rheological properties.The upwind control-volume Finite Volume Method (FVM) is applied to discretize the convection terms.The improved SIMPLE algorithm with velocity-free-surface coupled correction is developed to solve the equations on non-orthogonal,quadrilateral grids.The model is applied to simulate a debris flow event in Jiangjia Gully,Yunnan Province and to predict the flow pattern and bed erosion-deposition processes.The results show the effectiveness of the proposed numercial model in debris flow simulation and potential hazard analysis. | PENG Hong ZHAO Yanxin CUI Peng ZHANG Wanshun CHEN Xuejiao CHEN Xiaoqing | 2011 | Journal of Mountain Science2011，8，6： | 3 |
| 15 | Inconsistent creep between dendrite core and interdendritic region under different degrees of elemental inhomogeneity in nickel-based single crystal superalloys显示文摘The creep inconsistency between dendrite core and interdendritic region is investigated in a nickel-based single crystal superalloy under 1373 K and 137 MPa.Two specimens with higher and lower degree of elemental inhomogeneity on dendritic structures are compared.For specimen with higher inhomogeneity,stronger segregation of refractory elements reinforces the local strength in dendrite core,but damages the strength in interdendritic region.Creep strain is accumulated faster in interdendritic region giving rise to promoted dislocation shearing inγphase,faster degradation of dislocation networks and facilitated topological inversion of rated structures.Although the segregation of refractory elements produces a high density of topologically close-packed(TCP)phase in dendrite core,faster accumulation of creep strain forms microcracks prior in interdendritic region that gives rise to final rupture of the specimen.In another specimen,increased solid solution time gives rise to overall reduced inhomogeneity.Creep inconsistency is relieved to show more uniform evolution of dislocation substructures and rafting between dendrite core and interdendritic region.The second specimen is ruptured by formation and extension of microcracks along TCP phase although the precipitation of TCP phase is relatively restricted under reduced inhomogeneity.Importantly,the balance of local strength between dendrite core and interdendritic region results in over 40%increase of creep rupture life of the second specimen. | Wanshun Xia Xinbao Zhao Liang Yue Quanzhao Yue Jiangwei Wang Qingqing Ding Hongbin Bei Ze Zhang | 2021 | Journal of Materials Science & Technology2021，，33： | 2 |
| 16 | Numerical Simulation of Interaction Between Tributary Debris Flow and Main River显示文摘Based on two-phase flow theory and shallow water flow assumption,a mathematical model is applied to simulate debris flow.The model considers a two-phase mixture of sediments and water fluid.Assuming that the sediments and the water fluid move downstream with the same velocity,the flow of the mixture is described using a two dimensional depth averaged model with a unique 2-D momentum equation and two mass balance e- quations for the mixture and the sediments,respectively.The finite volume method is used f... | Zhang, Wanshun Cui, Peng Wang, Ying Qiao, Fei Wang, Xiekang | 2005 | 四川大学学报(工程科学版)2005，37，S1： | 2 |
| 17 | 基于转录组denovo拼接鉴定辣椒(Capsicum frutescens)中辣椒素生物合成相关基因显示文摘辣椒素是由辣椒果实产生的一组化合物,被广泛应用于许多领域,尤其是医药行业。辣椒素生物合成途径还没有被清楚地确定。为了解更多辣椒素生物合成的知识,我们对辣椒(Capsicum frutescens L.)胎座和果皮的混合材料进行了转录组测序。并使用不同的拼接软件对各拼接参数的影响进行了评估,最终获得了转录组数据de novo拼接的最佳策略之一。我们利用Trinity软件得到共54 045条高质量unigenes(转录物)。约92.65%的unigenes与马铃薯、番茄和辣椒(C.annuum)ESTs数据库公共蛋白序列和基因组有相似性。我们预测到了3个新的结构基因(DHAD,TD,PAT),填补了由Mazourek提出的辣椒素分子合成途径中的空缺,并且以KEGG(京东基因与基因组百科全书)分析为基础,鉴定到了参与辣椒素生物合成新的候选基因。大量的SSR(简单重复序列)和SNP(单核苷酸多态性)标记在C.frutescens和C.annuum序列中被预测到,这些标记可在识别辣椒群体多态性方面提供帮助。这些数据将为辣椒素生物合成途径及相关研究提供新的思路。此外,我们的转录组de novo拼接策略也可用于其他大量类似研究中。 | Liu Shaoqun Li Wanshun Wu Yimin Chen Changming Lei Jianjun 高凌云 孔秋生 | 2014 | 辣椒杂志2014，，1： | 2 |
| 18 | Protective effects of chitosan oligosaccharide and its derivatives against carbon tetrachloride-induced liver damage in mice显示文摘 | Yang Y Wanshun L Baoqin H | 2006 | Hepatol Res2006，35，3： | 1 |
| 19 | The effect of carboxymethyl -chitosan on proliferation and collagen secretion of normal and keloid skin fibroblasts显示文摘 | Chen Xiguang Wang Zhen Liu Wanshun | 2002 | Biomaterials2002，23，23： | 1 |
| 20 | Protective effects of chitosan oligosaccharide and its derivatives against carbon tetrachloride-induced liver damage in mice显示文摘 | Yang Y Wanshun L Baoqin H | 2006 | Hepatol Res2006，35，3： | 1 |
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