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| 1 | Serum semaphorin 4C as a diagnostic biomarker in breast cancer:A multicenter retrospective study显示文摘Background:To date,there is no approved blood-based biomarker for breast cancer detection.Herein,we aimed to assess semaphorin 4C(SEMA4C),a pivotal protein involved in breast cancer progression,as a serum diagnostic biomarker.Methods:We included 6,213 consecutive inpatients from Tongji Hospital,Qilu Hospital,and Hubei Cancer Hospital.Training cohort and two validation cohorts were introduced for diagnostic exploration and validation.A pan-cancer cohort was used to independently explore the diagnostic potential of SEMA4C among solid tumors.Breast cancer patients who underwent mass excision prior to modified radical mastectomy were also analyzed.We hypothesized that increased pretreatment serum SEMA4C levels,measured using optimized in-house enzymelinked immunosorbent assay kits,could detect breast cancer.The endpoints were diagnostic performance,including area under the receiver operating characteristic curve(AUC),sensitivity,and specificity.Post-surgery pathological diagnosis was the reference standard and breast cancer staging followed the TNM classification.There was no restriction on disease stage for eligibilities.Results:We included 2667 inpatients with breast lesions,2378 patients with other solid tumors,and 1168 healthy participants.Specifically,118 patients with breast cancer were diagnosed with stage 0(5.71%),620 with stage I(30.00%),966 with stage II(46.73%),217 with stage III(10.50%),and 8 with stage IV(0.39%).Patients with breast cancer had significantly higher serum SEMA4C levels than benign breast tumor patients and normal controls(P<0.001).Elevated serum SEMA4C levels had AUC of 0.920(95%confidence interval[CI]:0.900–0.941)and 0.932(95%CI:0.911–0.953)for breast cancer detection in the two validation cohorts.The AUCs for detecting early-stage breast cancer(n=366)and ductal carcinoma in situ(n=85)were 0.931(95%CI:0.916–0.946)and 0.879(95%CI:0.832–0.925),respectively.Serum SEMA4C levels significantly decreased after surgery,and the reduction was more striking after modified radical mastectomy,compared with mass excision(P<0.001).The positive rate of enhanced serum SEMA4C levels was 84.77%for breast cancer and below 20.75%for the other 14 solid tumors.Conclusions:Serum SEMA4C demonstrated promising potential as a candidate biomarker for breast cancer diagnosis.However,validation in prospective settings and by other study groups is warranted. | Ya Wang Long Qiao Jie Yang Xiong Li Yaqi Duan Jiahao Liu Shaoqi Chen Huayi Li Dan Liu Tian Fang Jingjing Ma Xiaoting Li Fei Ye Junxiang Wan Juncheng Wei Qin Xu Ensong Guo Ping Jin Mingfu Wu Lin Zhang Yun Xia Yaqun Wu Jun Shao Yaojun Feng Qing Zhang Zongyuan Yang Gang Chen Qinghua Zhang Xingrui Li Shixuan Wang Junbo Hu Xiaoyun Wang Mona P.Tan Kazuaki Takabe Beihua Kong Qifeng Yang Ding Ma Qinglei Gao | 2021 | Cancer Communications2021，41，12： | 3 |
| 2 | Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy显示文摘 | WU Qingli WANG Mingfu JAMES E | | 0，，： | 1 |
| 3 | Spontaneous movement of a retrotransposon generated genic dominant male sterility providing a useful tool for rice breeding显示文摘Male sterility in plants provides valuable breeding tools in germplasm innovation and hybrid crop production.However,genetic resources for dominant genic male sterility,which hold great promise to facilitate breeding processes,are extremely rare in natural germplasm.Here we characterized the Sanming Dominant Genic Male Sterility in rice and identified the gene SDGMS using a map-based cloning approach.We found that spontaneous movement of a 1978-bp long terminal repeat(LTR)retrotransposon into the promoter region of the SDGMS gene activates its expression in anther tapetum,which causes abnormal programmed cell death of tapetal cells resulting in dominant male sterility.SDGMS encodes a ribosome inactivating protein showing N-glycosidase activity.The activation of SDGMS triggers transcription reprogramming of genes responsive to biotic stress leading to a hypersensitive response which causes sterility.The results demonstrate that an ectopic gene activation by transposon movement can give birth to a novel trait which enriches phenotypic diversity with practical utility. | Conghao Xu Yifeng Xu Zhengji Wang Xiaoyu Zhang Yuying Wu Xinyan Lu Hongwei Sun Lei Wang Qinglu Zhang Qinghua Zhang Xianghua Li Jinghua Xiao Xu Li Mingfu Zhao Yidan Ouyang Xianbo Huang Qifa Zhang | 2023 | National Science Review2023，10，9： | 1 |
| 4 | Determination ofproanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy显示文摘 | Qingli Wu Mingfu Wang James E | 2003 | Anal Chem2003，75，： | 1 |
| 5 | Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods 显示文摘 | WANG Mingfu TADMOR Y WU Qingli | 2003 | Journal of Agricultural and Food Chemistry2003，51，21： | 1 |
| 6 | The contribution of photoinduced charge-transfer enhancement to the SERS of uranyl(Ⅵ) in a uranyl-Ag_2O complex显示文摘Charge-transfer(CT) is an important enhancement mechanism in the field of surface-enhanced Raman scattering(SERS) that typically increases the Raman intensity of molecules by as much as 10–100 times.Herein, a low-cost Ag_2O aggregates substrate was prepared via a facile chemical precipitation method,and the calculated CT-based enhancement factor of the uranyl ions adsorbed on it reached as high as 10~5, a metal-comparable value. The efficient photoinduced CT process from the valence band of Ag_2O to the LUMO of uranyl ions under appropriate excitation sources resulted in the repulsion of the axial oxygen atoms of the O=U=O bond, which enhanced its polarizability, creating a more intense Raman mode. To the best of our knowledge, this study firstly reports such a strong photoinduced CT enhancement of uranyl ions, with concentrations of 10^(-8) mol L^(-1) or lower being detected using this Ag_2O substrate. Most importantly, this research has shown that the photoinduced CT enhancement also contributes to the SERS of uranyl ions on pure Ag substrates which have often been ascribed to the electromagnetic enhancement in previous studies. In addition, Ag_2O can be used to selectively detect uranyl ions without interference from many other molecules or ions because of the energy matching rule of the photoinduced CT process, which was readily available for uranyl detection in the environmental aqueous solution. | Shaofei Wang Shanli Yang Haoxi Wu Jiaolai Jiang Lang Shao Yiming Ren Yingru Li Chuanhui Liang Mingfu Chu Xiaolin Wang | 2019 | Science Bulletin2019，64，5： | 1 |
| 7 | Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells显示文摘 | Qiong Li Mingfu Wu | 2008 | Cancer Letters2008，261，1： | 1 |
| 8 | Determination of isoflavones in red clover and related species by high -performance liquid chromatography combined with ultraviolet and mass spectrometric detection显示文摘 | Qingli Wu Mingfu Wang James E Simon | 2003 | Journal of Chromatography A2003，1016，： | 1 |
| 9 | HIF-1α promotes SARS-CoV-2 infection and aggravates inflammatory responses to COVID-19显示文摘Cytokine storm induced by Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)is a major pathological feature of Coronavirus Disease 2019(COVID-19)and a crucial determinant in COVID-19 prognosis.Understanding the mechanism underlying the SARS-CoV-2-induced cytokine storm is critical for COVID-19 control.Here,we identify that SARS-CoV-2 ORF3a and host hypoxia-inducible factor-1α(HIF-1α)play key roles in the virus infection and pro-inflammatory responses. | Mingfu Tian Weiyong Liu Xiang Li Peiyi Zhao Muhammad Adnan Shereen Chengliang Zhu Shanyu Huang Siyu Liu Xiao Yu Miaomiao Yue Pan Pan Wenbiao Wang Yongkui Li Xulin Chen Kailang Wu Zhen Luo Qiwei Zhang Jianguo Wu | 2021 | Signal Transduction and Targeted Therapy2021，6，9： | 1 |
| 10 | Overexpression of vimentin contributes to prostate cancer invasion and metastasis via src regulation 显示文摘 | Wei Juncheng Xu Gang Wu Mingfu | 2008 | Anticancer Res2008，28，： | 1 |
| 11 | Effects of Ganoderma on aging and learning and memory ability in senescence accelerated mice显示文摘 | WANG Mingfu CHAN Yinching WU Chinglein | 2004 | International Congress Series2004，1260，3： | 1 |
| 12 | High rate cycling performance of lanthanum-modified Li 4 Ti 5 O 12 anode materials for lithium-ion batteries显示文摘 | Ting-Feng Yi Ying Xie Qiuju Wu Haiping Liu Lijuan Jiang Mingfu Ye Rongsun Zhu | 2012 | Journal of Power Sources2012，，： | 1 |
| 13 | Determination of isoflavones in red dover and related species by high-performance liquid chromatography combined with ultraviolet and mass spectrometric detection显示文摘 | WU Qingli WANG Mingfu SIMON J E | 2003 | J Chromatogr A2003，1016，2： | 1 |
| 14 | Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy 显示文摘 | Wu Qingli Wang Mingfu James E | 2008 | Anal Chem2008，95，： | 1 |
| 15 | Determination of isoflavones in red clover and related species by high -performance liquid chromatography combined with ultraviolet and mass spectrometric detection显示文摘 | Qingli Wu Mingfu Wang James E Simon | 2003 | Journal of Chromatography A2003，1016，： | 1 |
| 16 | Hypoxia signaling in human health and diseases:implications and prospects for therapeutics显示文摘Molecular oxygen(O2)is essential for most biological reactions in mammalian cells.When the intracellular oxygen content decreases,it is called hypoxia.The process of hypoxia is linked to several biological processes,including pathogenic microbe infection,metabolic adaptation,cancer,acute and chronic diseases,and other stress responses.The mechanism underlying cells respond to oxygen changes to mediate subsequent signal response is the central question during hypoxia.Hypoxia-inducible factors(HIFs)sense hypoxia to regulate the expressions of a series of downstream genes expression,which participate in multiple processes including cell metabolism,cell growth/death,cell proliferation,glycolysis,immune response,microbe infection,tumorigenesis,and metastasis.Importantly,hypoxia signaling also interacts with other cellular pathways,such as phosphoinositide 3-kinase(PI3K)-mammalian target of rapamycin(mTOR)signaling,nuclear factor kappa-B(NF-κB)pathway,extracellular signal-regulated kinases(ERK)signaling,and endoplasmic reticulum(ER)stress.This paper systematically reviews the mechanisms of hypoxia signaling activation,the control of HIF signaling,and the function of HIF signaling in human health and diseases.In addition,the therapeutic targets involved in HIF signaling to balance health and diseases are summarized and highlighted,which would provide novel strategies for the design and development of therapeutic drugs. | Zhen Luo Mingfu Tian Ge Yang Qiaoru Tan Yubing Chen Geng Li Qiwei Zhang Yongkui Li Pin Wan Jianguo Wu | 2022 | Signal Transduction and Targeted Therapy2022，7，8： | 1 |
| 17 | Determination of proanthocyanidins in grape products by liquid chromatography/mass spectrometric detection under low collision energy显示文摘 | Qingli Wu Mingfu Wang James E | 2003 | Anal Chem2003，75，： | 1 |
| 18 | Cu_(1)-B dual-active sites catalysts for the efficient dehydrogenative coupling and CO_(2)electroreduction显示文摘Dual-active sites(DASs)catalysts have positive potential applications in broad fields because of their specific active sites and synergistic catalytic effects.Therefore,the controllable synthesis and finely regulating the activity of such catalysts has become a hot research area for now.In this work,we developed a pyrolysis-etching-hydrogen activation strategy to prepare the DASs catalysts involving single-atom Cu and B on N-doped porous carbon material(Cu_(1)-B/NPC).Numerous systematic characterization and density functional theoretical(DFT)calculation results showed that the Cu and B existed as Cu-N4 porphyrinlike unit and B-N_(3)unit in the obtained catalyst.DFT calculations further revealed that single-atom Cu and B sites were linked by bridging N atoms to form the Cu_(1)-B-N6 dual-sites.The Cu_(1)-B/NPC catalyst was more effective than the single-active site catalysts with B-N_(3)sites in NPC(B/NPC)and Cu-N4 porphyrin-like sites in NPC(Cu_(1)/NPC),respectively,for the dehydrogenative coupling of dimethylphenylsilane(DiMPSH)with various alcohols,performing the great activity(>99%)and selectivity(>99%).The catalytic performances of the Cu_(1)-B/NPC catalyst remained nearly unchanged after five cycles,also indicating its outstanding recyclability.DFT calculations showed that the Cu_(1)-B-N6 dual-sites exhibited the lowest energy profile on the potential energy surface than that of sole B-N_(3)and Cu-N4 porphyrin-like sites.Furthermore,the rate-limiting step of dehydrogenation of DiMPSH on Cu_(1)-B-N6 dual-sites also showed a much lower activation energy than the other two single sites.Benefitting from the superiority of the Cu_(1)-B-N6 dual-sites,the Cu_(1)-B/NPC catalyst can also be used for CO_(2)electroreduction to produce syngas.Thus,DASs catalysts are promising to achieve multifunctional catalytic properties and have aroused positive attention in the field of catalysis. | Konglin Wu Zhaobin Fang Cheng Peng Yining Zhang Binbin Jiang Yanshang Kang Zhiming Chen Mingfu Ye Yuxi Wu Xianwen Wei Shoujie Liu Sha Li Jian Zhang | 2023 | Nano Research2023，16，4： | 0 |
| 19 | NIR-Ⅱ fluorescence/photoacoustic imaging of ovarian cancer and peritoneal metastasis显示文摘Ovarian cancer is a global problem,and is typically diagnosed in the middle or late stages,with a mysterious abdominal mass or atypical abdominal metastases due to the lack of specific initial diagnostic methods.Dual-modal near-infrared Ⅱ(NIR-Ⅱ,1,000–1,700 nm)fluorescence/photoacoustic imaging has great potential in early ovarian cancer diagnosis and image-guided surgery due to its high sensitivity and deep penetration.Herein,we report a novel organic NIR-Ⅱ dye(H10)with excellent aggregation-induced-emission(AIE)characteristics(I/I0>1.6)utilizing a selenadiazolo-[3,4-f]benzo[c][1,2,5]thiadiazole(ST)-based building block.Then,water-soluble and biocompatible H10@follicle-stimulating hormone(H10@FSH)dots with superior optical/photoacoustic properties and a tenfold increase in ovarian-specific targeting ability were synthesized.Finally,for the first time,in vivo dual-mode NIR-Ⅱ fluorescent/photoacoustic(PA)imaging and image-guided surgery of patient-derived tumor xenograft(PDTX)and micro-metastatic abdominal ovarian cancer lesions were investigated.This novel strategy will establish a new method for early detection of ovarian cancer and significantly improve the prognosis of ovarian cancer patients. | Siyu Lu Liru Xue Meng Yang Jingjing Wang Yang Li Yuxin Jiang Xuechuan Hong Mingfu Wu Yuling Xiao | 2022 | Nano Research2022，15，10： | 0 |
| 20 | SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication显示文摘Viral infection in respiratory tract usually leads to cell death,impairing respiratory function to cause severe disease.However,the diversity of clinical manifestations of SARS-CoV-2 infection increases the complexity and difficulty of viral infection prevention,and especially the high-frequency asymptomatic infection increases the risk of virus transmission.Studying how SARS-CoV-2 affects apoptotic pathway may help to understand the pathological process of its infection.Here,we uncovered SARS-CoV-2 imployed a distinct anti-apoptotic mechanism via its N protein.We found SARS-CoV-2 virus-like particles(trVLP)suppressed cell apoptosis,but the trVLP lacking N protein didn’t.Further study verified that N protein repressed cell apoptosis in cultured cells,human lung organoids and mice.Mechanistically,N protein specifically interacted with anti-apoptotic protein MCL-1,and recruited a deubiquitinating enzyme USP15 to remove the K63-linked ubiquitination of MCL-1,which stabilized this protein and promoted it to hijack Bak in mitochondria.Importantly,N protein promoted the replications of IAV,DENV and ZIKV,and exacerbated death of IAV-infected mice,all of which could be blocked by a MCL-1 specific inhibitor,S63845.Altogether,we identifed a distinct anti-apoptotic function of the N protein,through which it promoted viral replication.These may explain how SARS-CoV-2 effectively replicates in asymptomatic individuals without cuasing respiratory dysfunction,and indicate a risk of enhanced coinfection with other viruses.We anticipate that abrogating the N/MCL-1-dominated apoptosis repression is conducive to the treatments of SARS-CoV-2 infection as well as coinfections with other viruses. | Pan Pan Weiwei Ge Zhiwei Lei Wei luo Yuqing Liu Zhanwen Guan Lumiao Chen Zhenyang Yu Miaomiao Shen Dingwen Hu Qi Xiang Wenbiao Wang Pin Wan Mingfu Tian Yang Yu Zhen Luo Xulin Chen Heng Xiao Qiwei Zhang Xujing Liang Xin Chen Yongkui Li Jianguo Wu | 2023 | Signal Transduction and Targeted Therapy2023，8，6： | 0 |
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