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| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | Association of gene and protein expression and genetic polymorphism of CC chemokine ligand 4 in colorectal cancer显示文摘BACKGROUND Leukocytes,such as T cells and macrophages,play an important role in tumorigenesis.CC chemokine ligand(CCL)4,which is produced by lymphocytes and macrophages,has been found to be expressed in the mucosa of the gastrointestinal tract and is a potent chemoattractant for various leukocytes.AIM To examine CCL4 expression and its genetic polymorphism rs10491121 in patients with colorectal cancer(CRC)and evaluate their prognostic significance.METHODS Luminex technology was used to determine CCL4 Levels in CRC tissue(n=98),compared with paired normal tissue,and in plasma from patients with CRC(n=103),compared with healthy controls(n=97).Included patients had undergone surgical resection for primary colorectal adenocarcinomas between 1996 and 2019 at the Department of Surgery,Ryhov County Hospital,Jönköping,Sweden.Reverse transcription quantitative PCR was used to investigate the CCL4 gene expression in CRC tissue(n=101).Paired normal tissue and TaqMan single nucleotide polymorphism assays were used for the CCL4 rs10491121 polymorphism in 610 CRC patients and 409 healthy controls.RESULTS The CCL4 protein and messenger RNA expression levels were higher in CRC tissue than in normal paired tissue(90%,P<0.001 and 45%,P<0.05,respectively).CRC tissue from patients with localized disease had 2.8-fold higher protein expression levels than that from patients with disseminated disease.Low CCL4 protein expression levels in CRC tissue were associated with a 30%lower cancer-specific survival rate in patients(P<0.01).The level of plasma CCL4 was 11%higher in CRC patients than in healthy controls(P<0.05)and was positively correlated(r=0.56,P<0.01)with the CCL4 protein level in CRC tissue.The analysis of CCL4 gene polymorphism rs10491121 showed a difference(P<0.05)between localized disease and disseminated disease in the right colon,with a dominance of allele A in localized disease.Moreover,the rate of the A allele was higher among CRC patients with mucinous cancer than among those with nonmucinous cancer.CONCLUSION The present study indicates that the CRC tissue levels of CCL4 and CCL4 gene polymorphism rs10491121,particularly in the right colon,are associated with clinical outcome in CRC patients. | Levar Shamoun Kalle Landerholm Amanda Balboa Ramilo Roland E Andersson Jan Dimberg Dick Wågsäter | 2021 | World Journal of Gastroenterology2021，27，30： | 2 |
| 3 | 卒中介入治疗培训指南:国际多学会共识文件显示文摘1背景
缺血性卒中是全球人口死亡和残疾的首要原因。很多急性大血管闭塞(emergent large vesselocclusion,ELVO)患者都会遗留长期残疾。事实上,这些颅内大动脉闭塞经常会导致大面积脑损伤,进而造成患者死亡或严重致残。 | Lavine SD Cockroft K Hoh B Bambakidis N Khalessi AA Woo H Riina H Siddiqui A Hirsch JA Chong W Rice H Wenderoth J Mitchell P Coulthard A Signh TJ Phatorous C Khangure M Klurfan P ter Brugge K Iancu D Gunnarsson T Pongpech S Rodesch G Soderman M Taylor A Krings T Orbach D Picard L Suh DC Zheng HQ Jansen O Muto M Szikora I Pierot L Brouwer P Gralla J Renowden S Andersson T Fiehler J Turjman F White P Januel AC Spelle L Kulcsar Z Chapot R Biondi A Dima S Taschner C Szajner M Krajina A Sakai N Matsumaru Y Yoshknura S Ezura M Fujinaka T Iihara K Ishii A Higashi T Hirohata M Hyodo A Ito Y Kawanishi M Kiyosue H Kobayashi E Kobayashi S Kuwayama N Matsumoto Y Miyachi S Murayama Y Nagata I Nakahara I Nemoto S Niimi Y Oishi H Satomi J Satow T Sugiu K Tanaka M Terada T Yamagami H Diaz O Lylyk P Jayaraman MV Patsalides A Gandhi CD Lee SK Abruzzo T Albani B Ansari SA Arthur AS Baxter BW Bulsara KR Chen M Almandoz JE Fraser JF Heck DV Hetts SW Hussain MS Klucznik RP Leslie-Mawzi TM Mack WJ McTaggart RA Meyers PM Mocco J Prestigiacomo CA Pride GL Rasmussen PA Starke RM Sunenshine PJ Tarr RW Frei DF Pabo M Nogueira RG Zaidat OO Jovin T Linfante I Yavagal D Liebeskind D Novakovic R Pongpech S 许岩 孙瑞 郭芮兵 | 2017 | 国际脑血管病杂志2017，25，5： | 2 |
| 4 | 显示文摘 | Péter Antal Torok Gabriella Armstrong Daniel W | 2000 | Journal of Chromatography A2000，904，： | 2 |
| 5 | Verfication of workflow task structures: a petri-net-based approach 显示文摘 | AALST van der W M P HOFSTEDE ter A H M | 2000 | Information Systems2000，25，1： | 1 |
| 6 | Evidence based selection of housekeeping genes显示文摘 | de Jonge H J Fehrmann R S de Bont E S Hofstra R M Gerbens F Kamps W A de Vries E G van der Zee A G te Meerman G J ter Elst A | | 0，，： | 1 |
| 7 | The Wnt/frizzled/GSK-3β pathway: a novel therapeutic target for cardiac hypertrophy显示文摘 | Blankesteijn W M de Schans V A M V ter Horst P | 2008 | Trends Pharmacol Sci2008，29，4： | 1 |
| 8 | Increased expression of leukotriene C4 synthase and predominant formation of cysteinyl-leukotrienes in human abdominal aortic aneurysm显示文摘 | Di Gennaro A W(a)gs(a)ter D M(a)yr(a)np(a)(a) MI | | 0，，： | 1 |
| 9 | Thermal processing of whole soya beans: Studies on the inactivation of antinutritional factors and effects on ileal digestibility in piglets显示文摘 | Qin G Ter Elst E R Bosch M W | 1996 | Anita Feed Sci Tech1996，57，： | 1 |
| 10 | YAWL:yet another workflow language 显示文摘 | ASLST W M P van der HOFSTEDE A H M ter | 2005 | Information Systems2005，30，1： | 1 |
| 11 | YAWL:Yet Another Workflow Language显示文摘 | W M P van der Aalst A H M ter Hofstede | 2003 | Accepted for publication in Information Systems and also available as QUT Technical report FIT-TR-2003-04 Queensland University of Technology Brisbane2003，2003，： | 1 |
| 12 | Workflow Patterns显示文摘 | W M P van der Aalst A H M ter Hofstede | 2003 | Distributed and Parallel Databases2003，14，1： | 1 |
| 13 | MMP-2 and MMP-9 are prominent matrix metalloproteinases during atherosclerosis development in the Ldlr(-/-)Apob(100/100) mouse显示文摘 | W(a)gs(a)ter D Zhu C Bj(o)rkegren J | 2011 | Int J Mol Med2011，28，2： | 1 |
| 14 | Modeling distribution of Amazonian tree species and diversity using remote sensing measurements 显示文摘 | Saatchi S Buermann W Steege H ter | 2008 | Remote Sensing of Environment2008，112，5： | 1 |
| 15 | Electrodeionization 1 :Migration of Nickel Ions Absorbed in a Rigid,Macroporous Cation-exchange Resin 显示文摘 | P B Spoor W R ter Veen L J J Janssen | 2001 | J Appl Electrochem2001，31，10： | 1 |
| 16 | Workflow patterns显示文摘 | VAN DER AALST W M P TER HOFSTEDE A H M KIEPUSZEWSKI B | 2003 | Distributed and Parallel Databases2003，14，1： | 1 |
| 17 | Workflow Patterns显示文摘 | van der Aalst W M P Ter Hofstede A H M Kiepuszewski B | 2003 | Distributed and Parallel Databases2003，14，3： | 1 |
| 18 | Modeling distribution of Amazonian tree species and diversity using remote sensing measurements显示文摘 | Saatchi S Buermann W Ter Steege H | | 0，，： | 1 |
| 19 | Ecosystem service supply and vulnerability to global change in Europe显示文摘 | Schr ter D Cramer W Leemans R | 2005 | Science2005，310，： | 1 |
| 20 | Workflow patterns 显示文摘 | Van DER AALST W M P TER HOFSTEDE A H M KIE- PUSZEWSKI B | 2003 | Distributed and Parallel Databases2003，14，1： | 1 |
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