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| 1 | Topological and evolutional relationships between HCV core protein and hepatic lipid vesicles: Studies in vitro and in conditionally transgenic mice显示文摘AIM: To investigate a specific association between hepatic steatosis and hepatitis C virus (HCV) core. METHODS: HeLa cells and primary mouse hepatocytes were transfected with HCV core plasmid, and conditional transgenics in which hepatic over-expression of HCV core is regulated by the tetracycline-off system, were developed. The expression of the HCV core was assessed over one to six months after withdrawal of doxycycline (dox) by immunohistochemistry (IHC) and Western blotting and by sequential liver biopsy. Hepatic steatosis was evaluated using oil red stain. 8-hydroxydeoxyguanosine (8-OHdG) stains and caspase levels were conducted to clarify hepatic oxidative stress and apoptosis rate. Serum aminotransferase was checked. RESULTS: The transfected hepatocytes had globular cores under the lipid vesicles. In transgenic mice on control diet, core expression was robust, localized to the cytoplasmic vesicle membrane and strongly associatedwith microvesicular steatosis, which was gradually replaced by macrovesicular steatosis. However, both steatosis and core positive hepatocytes diminished with time. Increases in aminotransferase, caspase and 8-OHdG were associated with peak core expression. CONCLUSION: The core protein was readily detected and morphologically associated with steatosis in individual hepatocytes both in vitro and in vivo. In vivo, oxidative stress caused by the core potentially reduced the number of core positive hepatocytes and in parallel the level of steatosis. To our knowledge, this is the f irst animal model that directly shows topological relationship between HCV core and hepatic lipid vesicles. | Ming-Ling Chang Jeng-Chang Chen Chau-Ting Yeh I-Shyan Sheen Dar-In Tai Ming-Yu Chang Cheng-Tang Chiu Deng-Yn Lin D Montgomery Bissell | 2007 | World Journal of Gastroenterology2007，13，25： | 4 |
| 2 | Acute respiratory distress syndrome in critically ill patients with severe acute respiratory syndrome 显示文摘 | Lew TW Kwek TK Tai D | 2003 | JAMA2003，290，： | 2 |
| 3 | Antibiotic Resistance in Salmonella Isolates from Imported Chicken Carcasses in Bhutan and from Pig Carcasses in Vietnam显示文摘 | Ellerbroek L Narapati D Tai N Phu Poosaran N Pinthong R Sirimalaisuwan A Tshering P Fries R Zessin K-h Baumann M Schroeter A | 2010 | Journal of Food Protection2010，，2： | 2 |
| 4 | Components of Hedyotis diffusa Wild显示文摘 | Tai D F Lin YM Chen F C | 1979 | Hua Hsueh1979，，3： | 1 |
| 5 | A Promising approach to con ductive patterns with high efficiency for flexible electronics 显示文摘 | Tai Y L Yang Z G Li Z D | 2011 | Appl Surf Sci2011，257，： | 1 |
| 6 | Treatmentof postoperative pain in oral and maxillofacial sur2gery显示文摘 | Coulthard P Haywood D Tai M A | 2000 | Br J OralMaxillofac surgery2000，38，： | 1 |
| 7 | Development of a 3D-printed external ventricular drain placement simulator: technical note 显示文摘 | Tai BL Rooney D Stephenson F | 2015 | J Neurosurg2015，123，4： | 1 |
| 8 | HCMV IE2-mediared inhibition of HAT activity downregulates P53 function显示文摘 | Hsu C H Chang M D Tai K Y | 2004 | EMBO J2004，23，： | 1 |
| 9 | Elastodynamic response of an interface crack in a layered composite under anti-plane shear impact load 显示文摘 | Li D H Tai W H | 1991 | Eng Fract Mech1991，39，5： | 1 |
| 10 | Synthesis and characterization of 6-O-acyl-2-O-alpha-D-glucopyranosyl-L-ascorbic acids with a branched-acyl chain显示文摘 | Tai A Kawasaki D Sasaki K | 2003 | Chem Pharm Bull (Tokyo)2003，51，2： | 1 |
| 11 | Large scale gene expression analysis of cholesterol-loaded macrophages显示文摘 | Shiffman D Mikita T Tai JT | 2000 | J Biol Chem2000，275，37： | 1 |
| 12 | In situ prepared transparent polyaniline electrode and its application in bifacial dyesensitized solar cells显示文摘 | Tai Q D Chen B L Guo F | | 0，，： | 1 |
| 13 | Vitamin C activity in guinea pigs of 6-O-acyl-2-O-alpha-D-glucopyranosyl-L-ascorbic acids with a branched-acyl chain显示文摘 | Tai A Kawasaki D Goto S | 2003 | Biosci Biotechnol Biochem2003，67，8： | 1 |
| 14 | Experimental approach to selecting H∞weighting functions for DC servos显示文摘 | Yang C D Tai H C Lee C C | 1997 | Journal of Dynamic Systems Measurement and Control1997，119，1： | 1 |
| 15 | Leech fotore- ceptors project their Galectin - containing processes into the optic neuropils where they contact AP cells 显示文摘 | Tai M H Rheuben M B Autio D M | 1996 | Comp Neurol1996，371，： | 1 |
| 16 | Envy as pain: Rethinking the nature of envy and its implications for employees and organizations 显示文摘 | Tai K Narayanan J McAllister D J | 2012 | The Academy of Management Review2012，37，1： | 1 |
| 17 | Angiotensin2 I converting enzyme ins- er2tion/deletion polymorphism and its association with diabetic nephrop- athy:a meta2analysis of studies reported between 1994 and 2004 and comp fi2 sing 14, 727 subjects 显示文摘 | Ng DP Tai BC Koh D | 2005 | Diabetologia2005，48，10： | 1 |
| 18 | Gene expression analysis by transcript profiling coupled to a gene database query 显示文摘 | Shimkets R A Lowe D G Tai J T | 1999 | Nat Biotechnol1999，17，8： | 1 |
| 19 | A cotton gene encodes a tonoplast aquaporin that is involved in cell tolerance to cold stress 显示文摘 | Li D D Tai F J Zhang Z T | 2009 | Gene2009，438，： | 1 |
| 20 | CD40 activation induces p53-dependent vascular endothelial growth factor secretion in human multiple myeloma celia显示文摘 | Tai YT Podar K Gupta D | 2002 | Blood2002，99，4： | 1 |
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