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| 1 | Regulation of clusterin/apolipoprotein J, a functional homologue to the small heat shock proteins, by oxidative stress in ageing and age-related diseases显示文摘 | Ioannis P. Trougakos Efstathios S. Gonos | 2006 | Free Radical Research2006，，12： | 2 |
| 2 | Ectopic expression of clusterin/apolipoprotein J or Bcl-2 decreases the sensitivity of HaCaT cells to toxic effects of ropivacaine显示文摘Local anesthetics inhibit cell proliferation and induce apoptosis in various cell types. Ropivacaine, a unique, novel tertiary amine-type anesthetic, was shown to inhibit the proliferation of several cell types including keratinocytes. We found that Ropivacaine could inhibit the proliferation and induce apoptosis in an immortalized human keratinocyte line, HaCaT, in a dose- and time-dependent manner and with the deprivation of serum. The dose-dependent induction of apoptosis by ropivacaine was demonstrated by DNA fragmentation analysis and the proteolytic cleavage of a caspase-3 substrate — poly (ADP-ribose) polymerase (PARP). In addition, ropivacaine downregulated the expression of clusterin/ apoliporotein J, a protein with anti-apoptotic properties, in a dose-dependent manner, which well correlated with the induction of apoptosis of HaCaT cells. To investigate the role of clusterin/apoliporotein J in ropivacaine-induced apoptosis, HaCaT cells overexpressing clusterin/apoliporotein J were generated and compared to cells expressing the well estab- lished anti-apoptotic Bcl-2 protein. Ectopic overexpression of the secreted form of clusterin/apoliporotein J or Bcl-2 decreased the sensitivity of HaCaT cells to toxic effects of ropivacaine as demonstrated by DNA fragmentation, the proteolytic cleavage of PARP and by a reduction in procaspase-3 expression. Furthermore, the downregulation of endogenous clusterin/apolipoprotein J levels by ropivacaine suggested that this might be one mechanism by which ropivacaine induced cell death in HaCaT cells. In conclusion, the ability of ropivacaine to induce antiproliferative responses and to suppress the expression of the anti-apoptotic protein clusterin/apolipoprotein J, combined with previ- ously reported anti-inflammatory activity and analgesic property of the drug, suggests that ropivacaine may have poten- tial utility in the local treatment of tumors. | Evangelos KONTARGIRIS Evangelos KOLETTAS Athina VADALOUCA Ioannis P. TROUGAKOS Efstathios S. GONOS Vasiliki KALFAKAKOU | 2004 | Cell Research2004，14，5： | 2 |
| 3 | Clusterin/apolipoprotein J in human aging and cancer显示文摘 | Trougakos IP Gonos ES | 2002 | Int J Biochem Cell Biol2002，34，： | 1 |
| 4 | Clusterin/apolipoprotein J in human aging and cancer 显示文摘 | Trougakos IP Gonos ES | 2002 | Int J Biochem Cell Biol2002，34，11： | 1 |
| 5 | Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex显示文摘 | Trougakos IP Lourda M Antonelou MH | | 0，，01： | 1 |
| 6 | Molecular effects of advanced glycation end products on cell signalling pathways, ageing and pathophysiology显示文摘 | O. Nedi&cacute S. I. S. Rattan T. Grune I. P. Trougakos | 2013 | Free Radical Research2013，，1： | 1 |
| 7 | Intracellular clusterin Inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein Complex显示文摘 | Trougakos IP Lourda M Antonelou MH | 2009 | Clin Cancer Res2009，15，1： | 1 |
| 8 | Functional analysis of elusterin/apolipoprotein J in cellular death induced by severe genotoxic stress显示文摘 | Trougakos I Gonos E | 2004 | Ann NY Acad Sci2004，1019，6： | 1 |
| 9 | Clusteri / apolipoprotein J in humanaging and cancer显示文摘 | Trougakos IP Gonos ES | 2002 | Int J Bioche Cell Biol2002，34，11： | 1 |
| 10 | Intracellular cluster - ininhibits mitochondrial apoptosis by suppressing p53 - activating stress signals and stabilizing the cytosolic Ku70 - Bax protein complex显示文摘 | TROUGAKOS IP LOURDA M ANTONELOU MH | 2009 | Clin Cancer Res2009，，1： | 1 |
| 11 | Functional analysis of clusterin/apolipoprotein J in cellular death induced by severe genotoxic stress 显示文摘 | Trougakos IP Gonos ES | 2004 | Annv N Y Acad Sci2004，1019，： | 1 |
| 12 | Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex显示文摘 | Trougakos IP Lourda M Antonelou MH | 2009 | Clin Cancer Res2009，15，1： | 1 |
| 13 | Differential effects of clusterin/apolipoprotein J on cellular growth and survival 显示文摘 | Trougakos 1P Lourda M Agiostratidou G | 2005 | Free Radic Bio Med2005，38，： | 1 |
| 14 | Intracellular clusterin inhibits mitochondrial apoptosis by suppressing p53-activating stress signals and stabilizing the cytosolic Ku70-Bax protein complex 显示文摘 | Trougakos IP Lourda M Antonelou MH | 2009 | Clin Cancer Res2009，15，1： | 1 |
| 15 | Differential effects of clusterin/apolipoprotein J on cellular growth and survival显示文摘 | TROUGAKOS IP LOURDA M Agiostratidou G | 2005 | Free Radic Biol Med2005，38，4： | 1 |
| 16 | Clusterin/apolipoprotein j in human aging and cancer显示文摘 | Trougakos IP Gonos ES | 2002 | Int J Biochem Cell Biol2002，34，11： | 1 |
| 17 | Regulation of clusterin/apolipoprotein J, a functional homologue to the small heat shock proteins, by oxidative stress in ageing and age-related diseases 显示文摘 | Trougakos IP Gonos ES | 2006 | Free Radic Res2006，40，12： | 1 |
| 18 | Cen- tral role of the proteasome in senescence and survival of hu- man fibroblasts: induction of a senescence - like phenotype upon its inhibition and resistance to stress upon its activa- tion 显示文摘 | Chondrogianni N Stratford FL Trougakos IP | 2003 | J Biol Chem2003，278，28： | 1 |
| 19 | Silencing expression of the clustetin / apolipoprotein gene in human cancer cells using small interfeting RNA induces spontaneous apoptosis,reduced growth ability, and cell sensitization to genotoxic and oxidative stress 显示文摘 | Trougakos IP So A Jansen B | 2004 | Cancer Res2004，64，5： | 1 |
| 20 | Advances and challenges in basic and translational research on clusterin显示文摘 | Trougakos IP Djeu JY Gonos ES | 2009 | Cancer Res2009，69，2： | 1 |
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