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| 1 | DNA barcoding provides distinction between Radix Astragali and its adulterants显示文摘Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species, DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A. membranaceus var. mongholica are commonly used as Radix Astragali in several Asian countries, including China, Japan, and Korea. However, in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore, a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study, we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, matK, rbcL, and coxI sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5% and 14.1%, respectively, whereas matK was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the matK sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and matK are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs. | GUO HaiYan1,2, WANG WeiWei2, YANG Ning2, GUO BaoLin3, ZHANG Sun2, YANG RuiJing2, YUAN Ye2, YU JunLin4, HU SongNian2, SUN QiShi1 & YU Jun2 1School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China 2Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China 3Chinese Academy of Medical Sciences, Peking Union Medical College Institute of Medicinal Plant Development, Beijing 100094, China 4Department of Chemistry, Tonghua Normal College, Tonghua 134002, China | 2010 | Science China(Life Sciences)2010，53，8： | 7 |
| 2 | On global behavior of the system of rational difference equations x_(n+1)=p+y_(n-k)/x_n,y_(n+1)=q+x_(n-k)/y_n显示文摘In this paper,we investigate the boundedness character,the global attractivity and the periodic nature of the system of rational difference equations:x_(n+1)=p+y_(n-k)/x_n,y_(n+1)=q+x_(n-k)/y_n,n=0,1,2…,where p>0,q>0,k∈{1,2,…} and the initial values xi,yi∈(0,∞),i=-k,-k+1,…,0. Some new results are obtained. | Xia An1,Tai-xiang Sun2,Yan-fang Zhang11. Department of Basic Courses,Institute of Disaster Prevention Science and Technology,Beijing 065201 2. Department of Mathematics,Guangxi University,Nanning 530004,China. | 2009 | Journal of Pharmaceutical Analysis2009，21，3： | 1 |
| 3 | Effect of cerebral lymphatic block on cerebral morphology and cortical evoked potential in rats显示文摘BACKGROUND: It has been shown that although brain does not contain lining endothelial lymphatic vessel,it has lymphatic drain.Anterior lymphatic vessel in brain tissue plays a key role in introducing brain interstitial fluid to lymphatic system;however,the significance of lymphatic drain and the affect on cerebral edema remains unclear.OBJECTIVE: To investigate the effect of cerebral lymphatic block on cerebral morphology and cortical evoked potential in rats.DESIGN: Randomized controlled animal study.SETTING: Institute of Cerebral Microcirulation of Taishan Medical College and Department of Neurology of Affiliated Hospital.MATERIALS:A total of 63 healthy adult male Wistar rats weighing 300-350 g were selected in this study.Forty-seven rats were used for the morphological observation induced by lymphatic drain and randomly divided into three groups:general observation group(n=12),light microscopic observation group(n=21)and electronic microscopic observation group(n=14).The rats in each group were divided into cerebral lymphatic block subgroup and sham-operation control subgroup.Sixteen rats were divided into cerebral the effect of cerebral lymphatic block on cortical evoked potential,in which the animals were randomly divided into sham-operation group(n=6)and cerebral lymphatic block group(n=10).METHODS:The experiment was carried out in the Institute of Cerebral Microcirculation of Taishan Medical College from January to August 2003.Rats in cerebral lymphatic block group were anesthetized and separated bilateral superficial and deep cervical lymph nodes under sterile condition. Superior and inferior boarders of lymph nodes were ligated the inputting and outputting channels, respectively, and then lymph node was removed so as to establish cerebral lymphatic drain disorder models. Rats in sham-operation control group were not ligated the lymphatic vessel and removed lymph nodes.and other operations were as the same as those in cerebral lymphatic block group. Morphological changes of the brain and alterations of latency of cortical evoked potential were detected on the 1st ,2nd 3rd ,5th ,7th ,10th and 15th .days after operation under general,light microscope and electronic microscope observations.MAIN OUTCOME MEASURES:①Cerebral morphological changes;②latent changes of cortical evoked potential.RESULTS:A total of 63 rats were involved in the final analysis.①Cerebral morphological changes:General observation showed that,for cerebral lymphatic block rats,the surface of brain was pale and full,and cerebral gyrus was wide and flattened sulci after cerebral lymphatic block; and cerebral tissue space prolongation,increased interstitial fluid, neuronal degeneration and necrosis,diffused phagocytes and satellitosis were observed under light microscope.Neuronal swell and necrosis,gilal cell swell,apparent subcellular changes such as mitochondron were observed under electronic microscope. ②Latent changes of cortical evoked potential:As compared with sham-operation control group.1atency of cortical evoked potential in cerebral lymphatic blockage group prolonged on the 5th day and 7th day after cerebral lymphatic block[(6.28±0.23),(6.97±0.35)ms;(6.23±0.22),(7.12±0.20)ms;P<0.01].CONCLUSION:①Cerebral lymphatic block plays an important role in cerebral morphology,and may result in abnormaliy of sensitive impulse conduction and prolong latency of cortical evoked potential.②Examination of cortical evoked potential is easy and convenient,so it is regarded as a key index for lymphatic disturbed cerebral injury. | Zuoli Xia1, Baoliang Sun2, Mingfeng Yang1, Dongmei Hu2, Tong Zhao2, Jingzhong Niu2 1Institute of Brain Microcirculation, Taishan Medical College, Taian 271000, Shandong Province, China 2Department of Neurology, Affiliated Hospital of Taishan Medical College, Taian 271000, Shandong Province, China | 2006 | Neural Regeneration Research2006，1，7： | 0 |
| 4 | Design, synthesis, biological activity and density function theory study of pyrazole derivatives containing 1,3,4- thiadiazole moiety显示文摘 | Xiaoming Ding Zhiwen Zhai Luping Lv Zhaohui Sun2, Xinghai Liu | 2017 | Frontiers of Chemical Science and Engineering2017，11，3： | 0 |
| 5 | Related factors of cognitive impairment after stroke显示文摘BACKGROUND: Cognitive impairment after stroke associates with various factors, such as age, educational years, etc. Besides concerning about the recovery of limb function after stroke, we should also focus on the rehabilitation of cognition. Moreover, we'd better pay attention to the control of all the risk factors of stroke, and improve the quality of life in stroke patients. OBJECTIVE: To analyze the factors that affect cognitive impairment after stroke. DESIGN: A related factors analysis. SETTINGS: Department of Neurology of Dalian Port Hospital and Dalian Second People's Hospital. PARTICIPANTS: Totally 148 stroke inpatients were selected from Department of Neurology, Dalian Port Hospital and Dalian Second People's Hospital from April 2004 to December 2005, including 100 males and 48 females, aging 45-75 years with an average age of (67±8) years; Their educational years ranged 2-10 years with an average of (6.1±3.7) years; The disease course ranged 15-30 days; All were right-handed. Inclusive criteria: All were accorded with the diagnostic standard set by the Fourth National Academic Meeting for Cerebrovascular Disease; Confirmed by CT or MRI; Informed consents were obtained from all the subjects. METHODS: After the disease conditions were stable, the patients were assessed with Wechsler memory scale (WMS) and Wisconsin card sorting test (WCST). WMS included forward and backward recitation of numbers and short-term memory (verbal memory, visual recognition). The number of times for correct and wrong classifications in WCST and the time to complete the trail making tests A and B were recorded. The focal volume, area and layer were recorded at 24 hours after admission. The general data of the patients were recorded, including name, sex, age, educational years, history of hypertension, history of diabetes mellitus. Electroencepalograph (EEG) was examined to record the wave shape, blood lipids were detected, and the cognition related indexes were analyzed with the Pearson correlation analysis. The correlation between cognitive indexes after stroke and the influencing factors were analyzed with multiple linear regression analysis. MAIN OUTCOME MEASURES: ① Correlation between cognitive indexes and imaging indexes; ② Influencing factors for cognitive indexes after stroke. RESULTS: All the 148 stroke patients were involved in the analysis of results. ① Correlation between cognitive indexes and CT indexes: There were obvious negative correlations between numerical symbol and focal layer (r =-0.234, P < 0.05), as well as between verbal memory and the focal area and volume (r =-0.363, -0.279, P < 0.05); Trail making test A had obvious positive correlation with focal area and volume (r =0.256, 0.256, P < 0.05). Results of multiple linear regression analysis: Correct classification was correlated with triglyceride (partial wave and θ wave (partial regression coefficient=0.231-0.908, P < 0.05); Verbal memory was correlated with EEG α wave, focal volume, sex, educational years and diabetes mellitus (partial regression coefficient=0.219-1.017, P < 0.05-0.01). Visual recognition had correlation with educational years and hypertension (partial regression coefficient=0.326, -1.163, P < 0.01). Trail making tests A and B were correlated with focal volume (partial regression coefficient=4.680, -18.561, P < 0.05). CONCLUSION: The factors that affect the cognitive function after stroke include sex, age, educational years, hypertension, diabetes mellitus, triglyceride, EEG wave shape, and the focal area, volume and layer. | Xiaofang Chen1, Yueji Sun2, Haiyan Liu1, Yunqiao Ding1, Shujuan Liu1, Jinghua Chen1, Xueying Wang1, Zhenpeng Han3, Fang Qu3 1Department of Neurology, Dalian Port Hospital, Dalian 116015, Liaoning Province, China 2Department of Psychology, Dalian Medical University, Dalian 116023, Liaoning Province, China 3 Department of Rheumatology, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China 4Department of Neurology, Dalian Second People’s Hospital, Dalian 116011, Liaoning Province, China | 2006 | Neural Regeneration Research2006，1，8： | 0 |
| 6 | Research on Intelligent Public Transport System Integrated IOT Elements显示文摘This paper will discuss how to provide a series of services,including the quick vehicle rescue,video surveillance & control,Real Time Localization,Real-Time Scheduling,Emergency Call,Information Services,Route Optimization Computing,and Historical path playback,to bus passengers or other customers,by using RFID and some other technologies related to IOT. | Xiangwen Li1,Wenfei Mu2,Mingguang Sang2andRuijuan Sun2 1 Associate Professor of Transportation Management College,Dalian Maritime University,Dalian,China P.O.Box 116026 2 Master student of Transportation Management College,Dalian Maritime University,Dalian,China P.O.Box 116026 | 2010 | 射频世界2010，，5： | 0 |
| 7 | Why Static O-H Bond Parameters Cannot Characterize the Free Radical Scavenging Activity of Phenolic Antioxidants: ab initio Study显示文摘The static O-H bond parameters including O-H bond length, O-H charge difference, O-H Mulliken population and O-H bond stretching force constant (k) for 17 phenols were calculated by ab initio method HF/6-31G**. In combination with the O-H bond dissociation enthalpies (BDE) of the phenols determined by experiment, it was found that there were poor correlationships between the static O-H bond parameters and O-H BDE. Considering the good correlationship between O-H BDE and logarithm of free radical scavenging rate constant for phenolic antioxidant, it is reasonable to believe that the ineffectiveness of static O-H bond parameters in characterizing antioxidant activity arises from the fact that they cannot measure the O-H BDE. | Hong Yu ZHANG1,2 *, You Min SUN2, De Zhan CHEN2 1Laboratory for Computational Biology, Zibo University, Zibo 255091 2Department of Chemistry, Shandong Teachers’ University, Jinan 250014 | 2001 | Chinese Chemical Letters2001，12，1： | 0 |
| 8 | APPLICATION OF GENETIC NEURAL NETWORK IN Zn-27 % Al-Mg ALLOY显示文摘A genetic neural net work m odel about design of Mg content in the alloy , based on tested databetw een Mg and tensile intensity or elongation in Zn 27 % Al alloy , has been established . Theresult has sho w n that the genetic neural netw ork is a better an d m ore applied method for m a terials design than the regress analysis . | L. S. Yang1) . H. T. Sun2) and A. N. Zhand2)1) Department of Materials Engineering , Luoyang Institute of Technology , Luoyang 471039 , China2) Department of Electric Engineering , Luoyang Institute of Technology , Luoyang 471039 , Ch | 1999 | Acta Metallurgica Sinica(English Letters)1999，12，5： | 0 |
| 9 | Force Parameter Model of 3-Roll Mills for Continuous Cold Rolling Wires显示文摘Fromthetimewhen3-rollmillsareinvelltedtotheendof1970's,theyhadbeenusedinhotrollingofwirerods,especiallyinproducingofnon-ferrousmetals.Itisestimatedthatapproximately90%ofthewirerodsmadefromaluminumandaluminumalloyswereproducedonthe3-rollmillsatthattim... | Baolong Zheng1 Li Sun2 Ruichen An2 Jin Wang1 Weichang Zhu1(l Material Science and Engineering School, University of Science and Technology Beijing, Beijing 100083, China2 Tangshan iron and Steel Co. Ltd., Tangshan 063000, China) | 1999 | International Journal of Minerals,Metallurgy and Materials1999，13，4： | 0 |
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