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| 1 | The ability of sperm selection techniques to remove single- or double-strand DNA damage显示文摘为精子的准备的许多技术当前是可得到的,哪个通常采用的大多数是密度坡度 centrifugation (DGC ) 和游泳起来(啜) 。迄今为止,这些方法看起来在为帮助繁殖技术(艺术) 选择功能的精子有效,但是他们可以在精子 DNA 上有否定效果。在这研究,处理技术消除单个海滨的包含的精子和双海滨 DNA 损坏的这些精液的能力被在 157 件精液样品的二尾巴的彗星试金和精子染色质分散测试从寻求帮助繁殖治疗的病人估计。我们显示的结果啜并且 DGC 在消除包含双海滨 DNA 损坏和精子与的精子是同等地有效的高度损坏了(降级) DNA 由存在描绘了单个海滨并且双海滨 DNA 裂缝。然而, DGC 比在选择从单个海滨的 DNA 损坏是免费的精子啜是更有效的。未来研究应该描述 DNA 损坏的各种各样的类型的重要性并且检验处理在每个实验室过去常决定他们的能力消除 DNA 损坏并且因此,经由艺术阻止基因变化的潜在的传播的协议的精子。 | Maria Enciso Miriam Iglesias Isabel Galan Jonas Sarasa Antonio Gosalvez Jaime Gosalvez | 2011 | Asian Journal of Andrology2011，13，5： | 2 |
| 2 | Comparison of ART outcomes in men with altered mRNA protamine 1/protamine 2 ratio undergoing intracytoplasmic sperm injection with ejaculated and testicular spermatozoa显示文摘Assisted reproductive technologies invoIving the use of spermatozoa and eggs for in vitro fertilization(IVF)have come as the solution for many infertile couples to become parents.However,in some cases,the use of ejaculated spermatozoa delivers poor IVF performance.Some studies have suggested the use of testicular spermatozoa in severe male in fertility cases,but no guideli nes regarding their utilization are currently available.In the present study,we found the mRNA protamine 1/protamine 2(P1/P2)ratio to be a valuable biomarker of poor sperm function that could be used as a diagnostic key for the identification of cases that would benefit from the use of testicular spermatozoa.A total of 23 couples undergoing egg donation cycles with at least one previous cycle failure were studied.All couples underwent two consecutive intracytoplasmic sperm injection(ICSI)cycles with either ejaculated or testicular spermatozoa(TESA).The sperm mRNA P1/P2 ratio,fertilization rate,blastocyst rate,and pregnancy and live birth rate were compared.Results showed improved ICSI and clinical outcomes in cycles with testicular spermatozoa in men with altered mRNA P1/P2 ratios.TESA cycles presented significantly higher rates of fertilization(mean±standard deviation:76.1%±15.1%vs 65.5%±18.8%),blastocyst formation(55.0%±20.3%vs 30.8%±23.8%),and good morphological quality blastocyst(28.9%±22.9%vs 13.5%±17.9%)and also improvements on pregnancy(60.9%vs 0%)and healthy birth rates(56.5%vs 0%)than EJACULATE cycles.The results described here suggest that in patients with previous IVF/ICSI failures and aberrant mRNA protamine ratios,the use of testicular spermatozoa may be a good alternative to improve clinical outcomes. | Jonas Sarasa Maria Enciso Laura Garcia Andrea Leza Klaus Steger Jon Aizpurua | 2020 | Asian Journal of Andrology2020，22，6： | 2 |
| 3 | TiO2-catalysed ozonation of raw Ebro river water显示文摘 | Cortes S Sarasa J | 2000 | Water Res2000，34，： | 1 |
| 4 | Study of the aromatic by-products formed from ozonation of anilines in aqueous solution显示文摘 | Sarasa J Cortés S Ormad P | 2002 | Water Research2002，36，： | 1 |
| 5 | TiO2-catalysed ozonation of raw Ebro river water 显示文摘 | Gracia R Corts S Sarasa J | 2000 | Wat Res2000，34，5： | 1 |
| 6 | TiO2-catalysed ozonation of raw Ebro river water显示文摘 | Gracia R Cortes S Sarasa J | 2000 | Water Res2000，34，5： | 1 |
| 7 | Study of the aromatic by-products formed from ozonation of anilines in aqueous solution显示文摘 | SARASA J CORTES S ORMAD P | 2002 | Water Res2002，36，12： | 1 |
| 8 | Characterization and photo-Fenton treatment of used tires leaehate显示文摘 | Sarasa J Liabres T Ormad P | 2006 | Journal of Hazardous Materials2006，136，3： | 1 |
| 9 | TiO2-catalysed ozonation of Raw Ebro river water显示文摘 | Gracia R S Cortes J Sarasa P | 2000 | Water Research2000，34，5： | 1 |
| 10 | Study of the aromatic by-products formed from ozonation of anilines in aqueous solution 显示文摘 | SARASA J CORTES S OSMAD P | 2002 | Water Research2002，36，12： | 1 |
| 11 | Comparative efficiency of the systems O3/high pH and O3/catalyst for the oxidation of chlorobenzenes in water显示文摘 | Sarasa J Ormad P | 2000 | Ozone:Science and Engineering2000，22，4： | 1 |
| 12 | Spinulosis of the face as a manifestation of demodicidosis显示文摘 | FARINA M C REQUENA L SARASA J L | 1998 | Br J Dermato1998，138，4： | 1 |
| 13 | TiO2-catalysed ozonation of raw river water显示文摘 | Gracia R Cortes S Sarasa J | 2000 | Water Res2000，34，5： | 1 |
| 14 | TiO2-catalysed ozonation of raw river water 显示文摘 | Gracia R Cortes S Sarasa J | 2000 | Water Research2000，34，5： | 1 |
| 15 | TiO2-catalysed ozonation of raw Ebro River water显示文摘 | Gracia R Cortes S Sarasa J | 2000 | Wat Res2000，34，5： | 1 |
| 16 | Ozonation of waste-water resulting from the production of organochlorine plaguicides derived from DDT and trichlorobenzene显示文摘 | ORMAD P PUIG A SARASA J | 1994 | Ozone Sci Eng1994，16，6： | 1 |
| 17 | TiO2 catalysed ozonation of raw Ebro river water 显示文摘 | GRACIA R CORTES S SARASA J | 2000 | Water Res2000，34，： | 1 |
| 18 | Oxidative and reductive pathways in manganese-catalyzed Fenton's reactions 显示文摘 | Watts R J Sarasa J Loge F J | 2005 | Journal of Environmental Engineering-Asce2005，131，1： | 1 |
| 19 | Expression of CD44 variant v6 in breast carcinoma and its relationship with other parameters of tumor biology 显示文摘 | Turrion F San Roman JM Sarasa JL Ortiz F Garcia R | 1998 | Med Clin (Barc)1998，111，5： | 1 |
| 20 | Analyzing the emotional outcomes of the online search behavior with search engines 显示文摘 | Blanco C F Sarasa R G Sanclemente C O | 2011 | Computers in Human Behavior2011，，1： | 1 |
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