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| 1 | Adding fifth dimension to optoacoustic imaging: volumetric time-resolved spectrally enriched tomography显示文摘Optoacoustics provides a unique set of capabilities for bioimaging,associated with the intrinsic combination of ultrasound-and light-related advantages,such as high spatial and temporal resolution as well as powerful spectrally enriched imaging contrast in biological tissues.We demonstrate here,for the first time,the acquisition,processing and visualization of five-dimensional optoacoustic data,thus offering unparallel imaging capacities among the current bioimaging modalities.The newly discovered performance is enabled by simultaneous volumetric detection and processing of multispectral data and is further showcased here by attaining time-resolved volumetric blood oxygenation maps in deep human vessels and real-time tracking of contrast agent distribution in a murine model in vivo. | Xose Luis Dean-Ben Daniel Razansky | 2014 | Light(Science & Applications)2014，3，1： | 5 |
| 2 | Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators显示文摘Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of neuroscience.We developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering brains.It can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm^(3) with respective spatial resolution of 35–200μm.Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate,for the first time,the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scattering brains.The newly developed platform thus offers unprecedented capabilities for functional whole-brain observations of fast calcium dynamics;in combination with optoacoustics'well-established capacity for resolving vascular hemodynamics,it could open new vistas in the study of neural activity and neurovascular coupling in health and disease. | X Luís Deán-Ben Gali Sela Antonella Lauri Moritz Kneipp Vasilis Ntziachristos Gil G Westmeyer Shy Shoham Daniel Razansky | 2016 | Light(Science & Applications)2016，5，1： | 5 |
| 3 | Transmission-reflection optoacoustic ultrasound(TROPUS)computed tomography of small animals显示文摘Rapid progress in the development of multispectral optoacoustic tomography techniques has enabled unprecedented insights into biological dynamics and molecular processes in vivo and noninvasively at penetration and spatiotemporal scales not covered by modern optical microscopy methods.Ultrasound imaging provides highly complementary information on elastic and functional tissue properties and further aids in enhancing optoacoustic image quality.We devised the first hybrid transmission–reflection optoacoustic ultrasound(TROPUS)small animal imaging platform that combines optoacoustic tomography with both reflection-and transmission-mode ultrasound computed tomography.The system features full-view cross-sectional tomographic imaging geometry for concomitant noninvasive mapping of the absorbed optical energy,acoustic reflectivity,speed of sound,and acoustic attenuation in whole live mice with submillimeter resolution and unrivaled image quality.Graphics-processing unit(GPU)-based algorithms employing spatial compounding and bent-ray-tracing iterative reconstruction were further developed to attain real-time rendering of ultrasound tomography images in the full-ring acquisition geometry.In vivo mouse imaging experiments revealed fine details on the organ parenchyma,vascularization,tissue reflectivity,density,and stiffness.We further used the speed of sound maps retrieved by the transmission ultrasound tomography to improve optoacoustic reconstructions via two-compartment modeling.The newly developed synergistic multimodal combination offers unmatched capabilities for imaging multiple tissue properties and biomarkers with high resolution,penetration,and contrast. | Elena Mercep Joaquin LHerraiz Xose Luis Dean-Ben Daniel Razansky | 2019 | Light(Science & Applications)2019，8，1： | 4 |
| 4 | Accounting for speed of sound variations in volumetric hand-held optoacoustic imaging显示文摘 | X. Luis DEAN-BEN Ali OZBEK Daniel RAZANSKY | 2017 | Frontiers of Optoelectronics2017，10，3： | 1 |
| 5 | High-sensitivity compact ultrasonic detector based on a pi-phase-shifted fiber Bragg grating显示文摘 | A Rosenthal D Razansky V Ntziachristos | 2011 | Opt Lett2011，36，10： | 1 |
| 6 | Multispectral Opto-acoustic Tomography of Deepseated Fluorescent Proteins in vivo 显示文摘 | DANIEL RAZANSKY MARTIN DISTE CLAUDIO VINEGONI | 2009 | Nature Photonics2009，3，： | 1 |
| 7 | Optoacoustic monitoring of cutting efficiency and thermal damage during laser ab- lation显示文摘 | Bay E Douplik A Razansky D | 2014 | Lasers Med Sci2014，29，： | 1 |
| 8 | 多谱线光声断层摄影术的癌症异质性光学成像显示文摘目的考察多谱线光声断层摄影术(MSOT)在展示目标外源物质的不均匀分布状况和活体样本恶性肿瘤中直径在毫米级的血管的特征等方面的能力。材料与方法实验中涉及的对动物的操作经上巴伐利亚行政区政府批准。研究人员使用一台MSOT装置(产生横断面图像速度为10帧/s,平面内分辨率约为150μm)对小鼠皮下肿瘤进行成像。为了研究动态对比强化,研究人员系统性地对3只患4T1型肿瘤的小鼠进行注射吲哚菁绿(ICG)操作,并在该操作前、注射时及注射后20 min,4 h和24 h对上述小鼠进行成像。荧光显微镜成像结果被用于对比。靶向荧光剂(注射6 h后)和血红蛋白氧合物的MSOT同时进行(4T1型肿瘤:n=3)。冰冻切片的荧光显微镜成像结果被用于验证上述结果。研究人员利用长循环时间金纳米棒对由于渗透性增加和肿瘤(4T1型肿瘤:n=4,HT29型肿瘤:n=3,A2780型肿瘤:n=2)内的滞留产生的积聚效应进行评估(注射前,注射时,注射后1 h,5 h和24 h)。暗场显微镜检查结果被用于验证上述结果。结果吲哚菁绿可用于动态对比强化。与荧光显微镜成像对比,MSOT显示了肿瘤内药剂的不均匀分布。靶向荧光剂和脱氧血红蛋白的同时成像让研究人员除目标药物摄取程度外,还可以了解肿瘤内的血管系统。久而久之在MSOT影像中看到的肿瘤中的金纳米棒的积聚过程,也呈异质性吸收。结论 MSOT技术可用于肿瘤的即时高空间分辨率观测,用以展示目标荧光染料、纳米材料以及肿瘤内部血管网的影像资料。 | Eva Herzog Adrian Taruttis Nicolas Beziere Andrey A.Lutich Daniel Razansky Vasilis Ntziachristos | 2012 | 中国医疗设备2012，27，9： | 1 |
| 9 | Molecular imaging by means of multisp-ectral optoacoustic tomography(MSOT)显示文摘 | Ntziachristos V Razansky D | 2010 | Chem Rev2010，110，5： | 1 |
| 10 | Molecular imaging by means of multispectral optoacoustic tomography (MSOT)显示文摘 | Ntziachristos V Razansky D | 2010 | Chem Rev2010，110，5： | 1 |
| 11 | Non-invasive determination of murine placental and foetal functional parameters with multispectral optoacoustic tomography显示文摘Despite the importance of placental function in embryonic development,it remains poorly understood and challenging to characterize,primarily due to the lack of non-invasive imaging tools capable of monitoring placental and foetal oxygenation and perfusion parameters during pregnancy.We developed an optoacoustic tomography approach for real-time imaging through entire ~4 cm cross-sections of pregnant mice.Functional changes in both maternal and embryo regions were studied at different gestation days when subjected to an oxygen breathing challenge and perfusion with indocyanine green.Structural phenotyping of the cross-sectional scans highlighted different internal organs,whereas multi-wavelength acquisitions enabled non-invasive label-free spectroscopic assessment of blood-oxygenation parameters in foeto-placental regions,rendering a strong correlation with the amount of oxygen administered.Likewise,the placental function in protecting the embryo from extrinsically administered agents was substantiated.The proposed methodology may potentially further serve as a probing mechanism to appraise embryo development during pregnancy in the clinical setting. | Kausik Basak Xose Luis Dean-Ben Sven Gottschalk Michael Reiss Daniel Razansky | 2019 | Light(Science & Applications)2019，8，1： | 1 |
| 12 | Multispectral opto- acoustic tomography of deep-seated fluorescent proteins in vivo显示文摘 | Razansky D Distel M Vinegoni C | 2009 | Nat Photonics2009，3，： | 1 |
| 13 | Real-time imaging of cardiovascular dynamics and circulating gold nanorods with multispectral optoacoustic tomography 显示文摘 | Taruttis A Herzog E Razansky D | 2010 | Opt Express2010，18，19： | 1 |
| 14 | Quantitative optoacoustic signal extraction using sparse signal representation显示文摘 | Rosenthal A Razansky D Ntziachristos V | 2009 | IEEE Trans Med Imaging2009，28，12： | 1 |
| 15 | Video rate optoaeoustic tomography of mouse kidney perfusion显示文摘 | Buehler A Herzog E Razansky D | 2010 | Opt Lett2010，35，14： | 1 |
| 16 | Near-field thermoacoustic tomography of small animals显示文摘 | KELLNBERGER S HAJIABOLI A RAZANSKY D | 2011 | Physics in Medicine and Biology2011，56，11： | 1 |
| 17 | Multispectral optoaeoustic tomography of matrix metalloproteinase activity in vulnerable human carotid plaques显示文摘 | Razansky D Harlaar N Hillebrands JL | 2012 | Mol Imaging Biol2012，14，3： | 1 |
| 18 | In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography显示文摘 | Vinegoni C Pitsouli C Razansky D | 2008 | Nat Methods2008，5，： | 1 |
| 19 | Mapping molecular agents distributions in whole mice hearts using Born-Normalized optical projection tomography显示文摘 | Vinegoni C Feruglio P F Razansky D | 2012 | Plos One2012，10，00： | 1 |
| 20 | Statistical optoacoustic image reconstruction using a-priori knowledge on the location of acoustic distortions显示文摘 | DEAN-BEN X L NTZIACHRISTOS V RAZANSKY D | 2011 | Applied Physics Letters2011，98，17： | 1 |
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