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| 1 | Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers显示文摘Rose has emerged as a model ornamental plant for studies of flower development, senescence, and morphology, as well as the metabolism of floral fragrances and colors.Virus-induced gene silencing(VIGS) has long been used in functional genomics studies of rose by vacuum infiltration of cuttings or seedlings with an Agrobacterium suspension carrying TRV-derived vectors. However, VIGS in rose flowers remains a challenge because of its low efficiency and long time to establish silencing. Here we present a novel and rapid VIGS method that can be used to analyze gene function in rose,called ‘graft-accelerated VIGS', where axil ary sprouts are cut from the rose plant and vacuum infiltrated with Agrobacterium. The inoculated scions are then grafted back onto the plants to flower and silencing phenotypes can be observed within 5 weeks, post-infiltration. Using this new method, we successfully silenced expression of the RhDFR_1, RhA G, and RhNUDX_1 in rose flowers, and affected their color, petal number, as well as fragrance, respectively. This grafting method will facilitate high-throughput functional analysis of genes in rose flowers. Importantly, it may also be applied to other woody species that are not currently amenable to VIGS by conventional leaf or plantlet/seedling infiltration methods. | Huijun Yan Shaochuan Shi Nan Ma Xiaoqian Cao Hao Zhang Xianqin qiu Qigang Wang Hongying Jian Ningning Zhou Zhao Zhang Kaixue Tang | 2018 | Journal of Integrative Plant Biology2018，60，1： | 11 |
| 2 | Projected Shifts in Kppen Climate Zones over China and Their Temporal Evolution in CMIP5 Multi-Model Simulations显示文摘Previous studies have examined the projected climate types in China by 2100. This study identified the emergence time of climate shifts at a 1?scale over China from 1990 to 2100 and investigated the temporal evolution of K o¨ppen–Geiger climate classifications computed from CMIP5 multi-model outputs. Climate shifts were detected in transition regions(7%–8% of China's land area) by 2010, including rapid replacement of mixed forest(Dwb) by deciduous forest(Dwa) over Northeast China, strong shrinkage of alpine climate type(ET) on the Tibetan Plateau, weak northward expansion of subtropical winterdry climate(Cwa) over Southeast China, and contraction of oceanic climate(Cwb) in Southwest China. Under all future RCP(Representative Concentration Pathway) scenarios, the reduction of Dwb in Northeast China and ET on the Tibetan Plateau was projected to accelerate substantially during 2010–30, and half of the total area occupied by ET in 1990 was projected to be redistributed by 2040. Under the most severe scenario(RCP8.5), sub-polar continental winter dry climate over Northeast China would disappear by 2040–50, ET on the Tibetan Plateau would disappear by 2070, and the climate types in 35.9%and 50.8% of China's land area would change by 2050 and 2100, respectively. The results presented in this paper indicate imperative impacts of anthropogenic climate change on China's ecoregions in future decades. | Duo CHAN Qigang WU Guixiang JIANG Xianglin DAI | 2016 | Advances in Atmospheric Sciences2016，33，3： | 10 |
| 3 | Inheritance of Perpetual Blooming in Rosa chinensis ‘Old Blush'显示文摘Perpetual blooming is one of the most important biological and economical traits in modern rose, while the genetic basis underlining the control of this trait is poorly investigated. With an aim in dissecting the genetic determinism of perpetual blooming, we developed six rose populations(OB, W, F1, F2, BC1 OB and BC1W) derived from a WOB population [interspecific diploid hybridization between Rosa chinensis ‘Old Blush'(OB) and R. wichuriana ‘Basye's Thornless'(W)]. Perpetual blooming is absent both in a F1 population with 296 individuals and a BC1 W population(W as the backcross parent) with 150 individuals. However, the perpetual blooming trait showed a typical 3︰1 segregation in a backcross population BC1 OB with OB as the backcross parent. In this population with 300 individuals, 83 plants had the perpetual blooming phenotype while the other 217 featured non-perpetual blooming, indicating that the perpetual blooming trait is very likely controlled by two recessive genes in R. chinensis(rpb1 and rpb2). These genetic data suggest that the inheritance of rose perpetual blooming may be controlled by a complex mechanism. | LI Shubin ZHOU Ningning ZHOU Qing YAN Huijun JIAN Hongying WANG Qigang CHEN Min QIU Xianqin ZHANG Hao WANG Shufang LI Shufa TANG Kaixue | 2015 | Horticultural Plant Journal2015，1，2： | 9 |
| 4 | Effects of closing and reopening live poultry markets on the epidemic of human infection with avian influenza A virus显示文摘Live poultry markets(LPMs) are crucial places for human infection of influenza A(H7N9 virus).In Yangtze River Delta,LPMs were closed after the outbreak of human infection with avian influenza A(H7N9) virus,and then reopened when no case was found.Our purpose was to quantify the effect of LPMs' operations in this region on the transmission of influenza A(H7N9) virus.We obtained information about dates of symptom onset and locations for all human influenza A(H7N9) cases reported from Shanghai,Jiangsu and Zhejiang provinces by May 31,2014,and acquired dates of closures and reopening of LPMs from official media.A two-phase Bayesian model was fitted by Markov Chain Monte Carlo methods to process the spatial and temporal influence of human cases.A total of 235 cases of influenza A(H7N9) were confirmed in Shanghai,Jiangsu and Zhejiang by May 31,2014.Using these data,our analysis showed that,after LPM closures,the influenza A(H7N9) outbreak disappeared within two weeks in Shanghai,one week in Jiangsu,and one week in Zhejiang,respectively.Local authorities reopened LPMs when there was no outbreak of influenza A(H7N9),which did not lead to reemergence of human influenza A(H7N9).LPM closures were effective in controlling the H7N9 outbreak.Reopening of LPM in summer did not increase the risk of human infection with H7N9.Our findings showed that LPMs should be closed immediately in areas where the H7N9 virus is confirmed in LPM.When there is no outbreak of H7N9 virus,LPMs can be reopened to satisfy the Chinese traditional culture of buying live poultry.In the long term,local authorities should take a cautious attitude in permanent LPM closure. | Jian Lu Wendong Liu Rui Xia Qigang Dai Changjun Bao Fenyang Tang Yefei Zhu Qiao Wang | 2016 | The Journal of Biomedical Research2016，30，2： | 7 |
| 5 | Numerical analysis and simulation of Czochralski growth processes for large diameter silicon crystals显示文摘Numerical analysis and simulation have been an effective means to develop the advanced growth technology and to control the defects type, size and density for silicon crystals of 300 mm and beyond. In the present paper, numerical analysis of the melt flow in the Czochralski (CZ) crystal growth configuration, the three dimensional (3D) modeling, the simulation of melt flow under the magnetic field, the inverse modeling and the time-dependent simulation are reviewed. Fi nally, comparison of numerical analysis with experimental measurements is discussed. | TU Hailing XIAO Qinghua GAO Yu ZHOU Qigang ZHANG Guohu CHANG Qing | 2007 | Rare Metals2007，26，6： | 5 |
| 6 | Dependence of nitrogen concentration in type Ib diamonds on synthesis temperature显示文摘Type Ib diamonds were grown by the temperature gradient method (TGM) at 5.5 GPa and 1500-1560 K in a china-type cubic anvil high pressure apparatus using Ni70Mn25Co5 alloy as solvent/catalyst. The concentration of nitrogen (CN) in type Ib diamonds synthesized at different synthesis temperatures was measured by a Fourier transform infrared (FTIR) spectrometer. The dependence of CN in diamond on synthesis temperature was studied. For the type Ib diamonds synthesized using Ni70Mn25Co5 as catalyst, its CN decreases along with the increase of synthesis temperature. | TIAN Yu JIA XiaoPeng ZANG ChuanYi LI ShangSheng XIAO HongYu ZHANG YaFei HUANG GuoFeng LI Rui HAN QiGang MA LiQiu LI Yong CHEN XiaoZhou ZHANG Chong MA HongAn | 2009 | Chinese Science Bulletin2009，54，9： | 5 |
| 7 | Experimental investigation on flow asymmetry in solid entrance region of a square circulating fluidized bed显示文摘To study the influence of back feeding particles on gas-solid flow in the riser, this paper investigated the flow asymmetry in the solid entrance region of a fluidized bed by particle concentration/velocity measurements in a cold square circulating fluidized beds (CFB). The pressure drop distribution along the riser and the saturation carrying capacity of gas for Geldart-B type particles were first analyzed. Under the condition of u0=4 m/s and Gs=21 kg/(m2 s), the back feeding particles were found to penetrate the lean gas-solid flow near the entrance (rear) wall before reaching the opposite (front) wall, thus leading to a relatively denser region near the front wall in the bottom bed. Higher solid circulation rate (u0=4 m/s, Gs=33 kg/(m2 s)) resulted in a higher particle concentration in the riser. However the back feeding particles with higher momentum increased the asymmetry of the particle concentration/velocity profile in the solid entrance region. Lower air velocity (u0=3.2 m/s) and Gs=21 kg/(m2 s), beyond the saturation carrying capacity of gas, induced an S-shaped axial solid distribution with a denser bottom zone. This limited the penetration of the back feeding particles and forced the fluidizing air to flow in the central region, thus leading to a higher solid holdup near the rear wall. Under the conditions of u0=4 m/s and Gs=21 kg/(m2 s), addition of coarse particles (dp=1145 m) into the bed made the radial distribution of solids more symmetrical. | Zhengyang Wang Shaozeng Sun Hao Chen Qigang Deng Guangbo Zhao Shaohua Wu | 2009 | Particuology2009，7，6： | 4 |
| 8 | Monitoring the Cultivated Slope Land in the Three Gorges Reservoir Area Based on Remote Sensing and GIS显示文摘By means of combining auto-extraction with manual interpretation, the current distribution information about cultivated land is obtained. The distribution information of 1992 is extracted from the dynamic polygons of 2002. The monitoring mini-system of the cultivated slope land is established. In the system, detailed surveys, focused on the resources of cultivated slope land, are carried out. The results indicate that the area of the cultivated slope land is very large. Meanwhile, there are lots of cultivated steep slopes with gradient above 35°.The areas of steep land cultivated had been slowly reduced from 1992 to 2002. At the same time, the pressures of returning farm land to forestry are great in all counties. The conflicts between population growth, insufficient grain supply and stagnant economic development sharpen increasingly. It is inevitable to improve the agricultural structure. | ZHOU Jieming1, ZHOU Qigang 2, 3 ,HUANG Zhiqin 2, 3, 4 1. The Faculty of Geography and Resources Science, Sichuan Normal University, Chengdu 610066, Sichuan, China 2. Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China 3. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China 4. Information Center of Land and Resources Department of Sichuan Province, Chengdu 610041, Sichuan, China | 2006 | Wuhan University Journal of Natural Sciences2006，11，4： | 4 |
| 9 | Comparison of measurements and simulation results in 300mm CZ silicon crystal growth显示文摘A special thermal modeling tool, CrysVUn, which was developed by Crystal Growth Laboratory (CGL) of Fraunhofer Institute of Integrated Systems and Devices Technology in Erlangen of Germany, was used for numerical analysis of growth interface situation. The heat transportation, argon flow and melt convection have been considered. Cauchy's first and second laws of motion have been the governing partial equations for stress calculation. The measurement results and simulation results were compared and the interface shape and thermal stress distribution during 300 mm Czochralski (CZ) silicon crystal growth with different growth rates were predicted. | GAO Yu TU Hailing ZHOU Qigang DAI Xiaolin XIAO Qinghua | 2007 | Rare Metals2007，26，6： | 3 |
| 10 | LUCC and Accompanied Soil Degradation in China from 1960's to 1990's显示文摘This paper tries to qualitatively analyze land use and cover changes (LUCC) and accompanied soil degradation in China, the data of World Atlas of Agriculture in 1969, Land Cover Data of Asia in 1992 and Global Assessment of Human Induced Soil Degradation in 1992, etc. have been used. From 1960' s to 1990's, the area of forestland had increased, arable land and paddy as well as grassland had significantly decreased in China. The major type of soil degradation is due to water erosion, which is widely distributed especially in Loess Plateau and in Southeast and Southwest China, this happened in forestland, grassland and arable land and mainly resulted from deforestation. The secondary type is wind erosion, which is mainly distributed in Northwest China, it happened in arable land and grassland and was caused by overgrazing. Chemical deterioration is distributed in North and Northwest China, which happened in paddy, arable land and grassland. Physical deterioration only concentrated in local area in North China, it happened in arable and paddy. Chemical and physical deteriora-tion mainly resulted from unreasonable activity. Because of different human activities, different LUCC and different natural conditions, the types and strength of soil degradation can be different. | JIANG Qigang(College of Earth Sciences, Jilin University, Changchun 130026, P. R. China) Shintaro GOTO and Hiroki TAKAMURA(Faculty of Geoenvironmental Science, Rissho University, Saitama, 360-0194, Japan) | 2002 | Global Geology2002，7，1： | 3 |
| 11 | Effect of thermal shield and gas flow on thermal elastic stresses in 300mm silicon crystal显示文摘The thermal elastic stresses induced in 300 mm Si crystal may be great troubles because it can incur the generation of dislocations and undesirable excessive residual stresses. A special thermal modeling tool, CrysVUn, was used for numerical analysis of thermal elastic stresses and stress distribution of 300 mm Si crystal under the consideration of different thermal shields and gas flow conditions. The adopted governing partial equations for stress calculation are Cauchy′s first and second laws of motion. It is demonstrated that the presence and shape of thermal shield, the gas pressure and velocity can strongly affect von Mises stress distribution in Si crystal. With steep-wall shield, however, the maximal stress and ratio of high stress area are relatively low. With slope-wall shield or without shield, both maximal stress and ratio of high stress area are increased in evidence. Whether thermal shields are used or not, the increase of gas flow velocity could raise the stress level. In contrast, the increase of gas pressure cannot result in so significant effect. The influence of thermal shield and gas flow should be attributed to the modification of heat conduction and heat radiation by them. | GAO Yu XIAO Qinghua ZHOU Qigang DAI Xiaolin TU Hailing | 2006 | Rare Metals2006，25，z2： | 2 |
| 12 | Remote Sense and GIS-Based Division of Landslide Hazard Degree in Wanzhou District of the Three Gorges Reservoir Area显示文摘An evaluation model divided landslide hazard degrees in Wanzhou District of Three Gorges Reservoir Area. The model was established by GIS techniques and took land use/cover, stratum characters, slope aspect, slope gradient, elevation difference and slope shape as evaluation factors. The data of land use/cover were obtained by remote sensing, and the weights of the factors mentioned above were established by the analytic hierarchy process (AHP). The results indicate, low danger areas in the studied area account for 66.51%, and high danger areas and very high danger areas occupy 1/3 of the total area. The regions of high and very high danger are mainly located around the urban area of Wanzhou District and on the banks of the Yangtze River with a relatively large area , where collapse and landslide directly threats densely populated areas and Three Gorges Reservoir. Slope destabilization, if occurs, will bring huge loss to social economy. All research results are consistent with the actual conditions; therefore, they can be regarded as a useful basis for planning and constructing of the reservoir area. | ZHOU Qigang FENG Wenlan SONG Shujun YUAN Lifeng ZHOU Wancun | 2006 | Wuhan University Journal of Natural Sciences2006，11，4： | 1 |
| 13 | Detection of human norovirus in cherry tomatoes,blueberries and vegetable salad by using a receptor-binding capture and magnetic sequestration(RBCMS)method显示文摘 | Pan Liangwen Zhang Qigang Li Xiang | 2012 | Food Microbiol2012，30，2： | 1 |
| 14 | The number of independent sets of tricyclic graphs显示文摘 | Zhu Zhongxun Yu Qigang | 2012 | Applied Mathematics Letters2012，25，10： | 1 |
| 15 | Enhanced Cata- lytic Activity of Hemoglobin in Organic Solvents by Lay-ered Titanate Immobilization显示文摘 | Qigang Wang Qiuming Gao Jianlin Shi | 2004 | Journal of the American Chemical Society2004，126，14： | 1 |
| 16 | Finite element analysis of high-pressure anvils according to the principle of lateral support显示文摘 | Han Qigang Ma Hongan Li R | 2007 | Journal of Applied Physics2007，，102： | 1 |
| 17 | Overexpression of the mTERT gene by adenoviral vectors promotes the proliferation of neuronal stem cells in vitro and stimulates neurogenesis in the hippocampus of mice显示文摘We sought to construct the adenoviral vector carrying the gene encoding mouse telomerase reverse transcriptase(mTERT),as well as detect its expression and effect on the proliferation of neuronal stem cells.mTERT was am-plified by RT-PCR and then the eukaryotic expression vector of pDC-EGFP-TERT was constructed.After DNA sequence analysis,we detected that there were 293 cells transfected with pDC-EGFP-TERT and helper adenovirus plasmid pBHG lox ΔE1,and three Cre using Lipofectamine 2000 mediation,named Ad-mTERT-GFP,to pack-age adenoviral particles.The Ad-mTERT-GFP was used to infect neuronal stem cells and then the expression and activity of mTERT were detected.In addition,Bromodeoxyuridine labeling test identified the impact of mTERT overexpression on proliferation of neuronal stem cells.The recombinant adenoviral vector confirmed that mTERT was successfully constructed.Overexpression of mTERT stimulated the proliferation of neuronal stem cells both in vitro and in vivo.mTERT overexpression via adenoviral vector carrying mTERT cDNA upregulated the ability of proliferation in neuronal stem cells. | Mengying Liu Yao Hu Lijuan Zhu Chen Chen Yu Zhang Weixiang Sun Qigang Zhou | 2012 | The Journal of Biomedical Research2012，26，5： | 1 |
| 18 | High-water-content mouldable hydrogels by mixing clay and a dendritic molecular binder显示文摘 | Wang Qigang Justin LMynar Masaru Yoshida | 2010 | Nature2010，463，7279： | 1 |
| 19 | Selecting activated carbon for water and wastewater treatment studies显示文摘 | ZHANG Wei CHANG Qigang LIU Wandong | 2007 | Environmental Progress2007，26，3： | 1 |
| 20 | Improved methods for carbon adsorption studies for water and wastewater treatment 显示文摘 | YING Weichi ZHANG Wei CHANG Qigang | 2006 | Environmental Progress2006，25，2： | 1 |
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