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| 1 | An analysis of 10218 ulcerative colitis cases in China显示文摘AIM: To analyze the characteristics of ulcerative colitis(UC)in Chine.METHODS: From 1981 to 2000, a total of 10218 patients of UCreported in Chinese medical literature and including ourcases diagnosed were analyzed according to the diagnosticcriteria of Lennard-Jones.RESULTS: The number of cases increased by 3.08 times overthe past 10 years (2506 patients were diagnosed from 1981 to1990 while 7512 patients were diagnosed from 1991 to 2000).Lesion renge were described in 7966 patients, 5592 (70.20%)were proctosigmoiditis or proctitis, 1792(22.50%) left-sidedcolitis, 582(7.30%) pancolitis. Among the 8122 patients,2826 (34.8%) had first episole, 4272 (52.6%) had chronicrelapse, 869 (10.7%) were of chronic persist type, 154(1.9%) were of acuts fulminant tyle. The course of theillress were described in 5867 patients, 4427(75.5%) wereless than 5 years, 910 (15.5%) between 5 and 10 years, 530(9.1%) more then 10 years. Six hundred and sixteenpatients patients (6.1% ) had extraintestinal manifestations.The mean age at the diagnosis was 40.7 years( range 6-8 years, and the peak ages 30-49 years). The male to femaleratio was1.09. Among 270 patients diagnosed in ourhospital, 36 had histories of smoking, there was no negativeassociation between the severity of UC and smoking ( P >0.05), 21 smokers were followed up for one year, 15 of themhad given up smoking when the disease were diagnosed,and one year later, 7 patients relapsed, another 6 patientscontinued smoking, and one year later, 2 patients relapsed.Among 270 UC patients diagnosed in our hospital, 4 patients(1.48%) from 2 families had familial history of UC.Treatment was mentioned in 6859 patients, only SASP and/or corticosteroid only in 1276 patients(18.6%), only Chineseherbs in 1377 patients (20.1%), combined Chinese andwestern medicine in 4056 patients (59.1%), surgery wasperformed in 87 patients (1.3%), other treatments in 63patients(0.9%).CONCLUSIONS: In China, number of UC patients increasedsignificantly in the past 10 years. Lesions are commonlylocated to left side colon. The course is short with rereextraintestinal manifestations. The age of onset is relativelyhigh. Males and females are nearly equally affected. Nonegative relation was found between smoking and severityof the disease. Familial relatives are rarely involved.Traditional Chinese medicine (TCM) is widely used in thetreatment of UC. | Xue-Liang Jiang Department of Gastroenterology,Chinese PLA General Hospital of Jinan Command,Jinan 250031,China Hui-Fei Cui Department of Biochemical Pharmaceutics,Shandong University,Jinan 250012,Shandong Province,China | 2002 | World Journal of Gastroenterology2002，8，1： | 217 |
| 2 | Challenges in Research and Development of Traditional Chinese Medicines显示文摘This review is briefly to recall the history of research and development (R&D) of Chinese materia medica (CMM) and to discuss the challenges of Chinese traditional and herbal medicines (CTHM) facing the modern science and technology. The R&D of CTHM is thought to be an important pathway for new drug discovery. Since1949, about 140 approved new drugs have been developed, among which about 80 originated directly or indirectly from medicinal plants. CTHM has gained interest from the international medical, biomedical, and pharmaceutical institutions as a valuable source of potential medicines. For the modernization of CMM and innovative research of CTHM, there are following challenges to be faced: (1) to evaluate the efficacy, pharmacological properties, action mechanism, and active chemical constituents; (2) to develop new methodologies for the quality and safety of CTHM; (3) to apply new '-omics' techniques to accelerate drug discoveries developed from CTHM; and (4) to apply international practices including good agricultural practice, good manufacturing practice, good laboratory practice, and good clinical practice in the R&D of CTHM. | LIU Chang-xiao1*, XIAO Pei-gen2, PENG Yong2, SONG Nai-ning1 1. Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China | 2009 | Chinese Herbal Medicines2009，1，1： | 34 |
| 3 | Epigenetic regulation: methylation of histone and non-histone proteins显示文摘Histone methylation is believed to play important roles in epigenetic memory in various biological processes. However, questions like whether the methylation marks themselves are faithfully transmit- ted into daughter cells and through what mechanisms are currently under active investigation. Previ- ously, methylation was considered to be irreversible, but the recent discovery of histone lysine de- methylases revealed a dynamic nature of histone methylation regulation on four of the main sites of methylation on histone H3 and H4 tails (H3K4, H3K9, H3K27 and H3K36). Even so, it is still unclear whether demethylases specific for the remaining two sites, H3K79 and H4K20, exist. Furthermore, be- sides histone proteins, the lysine methylation and demethylation also occur on non-histone proteins, which are probably subjected to similar regulation as histones. This review discusses recent pro- gresses in protein lysine methylation regulation focusing on the above topics, while referring readers to a number of recent reviews for the biochemistry and biology of these enzymes. | LAN Fei1 & SHI Yang 2 1 Department of Biology, Constellation Pharmaceuticals, 148 Sidney Street, Cambridge, MA 02139, USA 2 Department of Pathology, Harvard Medical School, 77 Ave Louise Pasteur, Boston MA, 02115, USA | 2009 | Science China(Life Sciences)2009，52，4： | 27 |
| 4 | Influence of Different Extraction and Purification Methods on Astragalus Polysaccharides and Pharmacological Evaluation显示文摘Objective To clarify the influence on component and pharmacological action of Astragalus polysaccharides(APS) as complementary therapeutic agents prepared by different extraction and purification techniques.Methods Components of APS prepared by different extraction and purification techniques were analyzed,and these APS were used for synergy and attenuation of chemotherapy,radiotherapy treatment with H22 liver cancer and Lewis lung cancer of tumor-bearing mice,and also used for the regulation of immune function to immunosuppression mice.Results Experimental data were analyzed by means of statistical method to get pharmaco-result:A3(extracted by microwave assistance and purified by membrane separation)>A4(extracted by refluxing and purified by membrane separation)>A1(extracted by refluxing and no purification)≈A2(extracted by microwave assistance and no purification).There were no significant differences on pharmacodynamic action between A1 and A2.However,compared with A1 and A2,it was worth noting that A3 and A4 exhibited good pharmacodynamic action.Then A3-in and A4-in,the samples in dialyzer after dialysis,were separated and purified to get homogeneous APS,which were the principal constituents of APS in dialyzer,with the molecular weight(Mw) of 7669 and 14142 determined by HPGPC,respectively.The average Mw of APS outside of the dialyzer,A3-out was 3102 and A4-out 3256,which were the main compositions of A3 and A4,accounted for 79.63% and 53.92%,respectively.Conclusion APS with Mw about 5000 Da exhibit better antitumor effect and immunological activity.Refluxing,microwave assistance extractions,and membrane enrichment techniques bring different cases on Mw distribution,components and pharmacodynamic action,and obviously exhibit relationship among component,Mw distribution,and pharmacological action. | YANG Yi-fang1,FENG Jing-qian1,XU Hai-yan1,PENG Dan-ming2,HU Jian-ping2 1.Shanghai Institute of Pharmaceutical Industry,Shanghai 200040,China 2.Jiangxi Provincial Academy of Traditional Chinese Medicine,Nanchang 330077,China | 2010 | Chinese Herbal Medicines2010，2，1： | 24 |
| 5 | Glutamine synthetase as an early marker for hepatocellular carcinoma based on proteomic analysis of resected smal hepatocel ular carcinomas显示文摘BACKGROUND:Hepatocellular carcinoma(HCC)is a highly malignant tumor with a poor prognosis.Because small HCCs possess most of the characteristics of early HCC,we investigated small HCCs to screen potential biomarkers for early diagnosis.METHODS:Proteins were extracted from 10 sets of paired tissue samples from HBV-infected small-HCC patients.The extracted proteins were well resolved by two-dimensional electrophoresis.These HCC-associated proteins were then identified by MALDI-TOF/TOF MS following image analysis.Western blotting and immunohistochemistry were used to assess glutamine synthetase(GS)and phenazine biosynthesislike domain-containing protein(PBLD)expression in liver tissue.Enzyme-linked immunosorbent assays in 152 serum samples(from 49 healthy donors,24 patients with liver cirrhosis,and 79 with HCC)were used to further assess the significance of GS clinically.RESULTS:Fifteen up-regulated and three down-regulated proteins were identified.Western blotting confirmed GS overexpression and decreased PBLD expression in liver tissue.Immunohistochemistry showed that GS was expressed in 70.0%(84/120)of HCCs and 35.8%(43/120)of nontumor tissues;PBLD was expressed in 74.2%(89/120) of nontumor tissues and 40.8%(49/120)of HCCs.The Chi-square test showed significant expression differences between HCCs and adjacent tissues.Consistent with this,serum GS levels in HCC patients were significantly higher than those in liver cirrhosis patients and healthy donors,while the latter two groups were also significantly different.In addition, a diagnostic cutoff value of 2.6 mg/ml was used for GS;it was elevated in 19(76.0%)of 25 HCC patients with AFP≤20 ng/ml and 47(88.7%)of 53 HCC patients with AFP≤200 ng/ml.CONCLUSION:GS and PBLD are abnormally expressed in most HCCs.GS may be a novel serum marker for early HCC, especially for those patients with low AFP levels(≤200 ng/ml). | Jiang Long,Zhen-Wei Lang,Hua-Guang Wang,Tai-Ling Wang,Bao-En Wang and Si-Qi Liu Liver Research Center,Beijing Friendship Hospital, Capital Medical University,Beijing 100050,China Department of Hepatology,Department of Pathology, Beijing Youan Hospital,Capital Medical University,Beijing 100069,China Department of Pharmaceutical Affairs,Beijing Chaoyang Hospital,Capital Medical University,Beijing 100020,China Department of Pathology,China-Japan Friendship Hospital,Beijing 100029,China Beijing Genomics Institute,Chinese Academy of Science,Beijing Airport Industrial Zone B-6,Shunyi,Beijing 101300,China | 2010 | Hepatobiliary & Pancreatic Diseases International2010，9，3： | 21 |
| 6 | Transformation of Compound K from Saponins in Leaves of Panax notoginseng by Immobilized β-Glucanase显示文摘Objective To prepare an active anti-tumor component,compound K(C-K),from saponins in leaves of Panax notoginseng(SLPN) using immobilized β-glucanase.Methods Two entrapments,alginate gel-1(Alg 1) and alginate gel-2(Alg 2),were evaluated for their ability to immobilize β-glucanase.The amount and purity of C-K obtained from the transformation process were analyzed by HPLC,and the immobilizing parameters were optimized.Results β-Glucanase can be immobilized and reused with either of the entrapment.However,using Alg 1 resulted in higher enzyme activity than Alg 2.The optimal concentration of the immobilized enzyme was 10%;The optimal crosslinking time was 4-6 h;and the optimal concentration of the crosslinking agent was 6%- 7%.Conclusion Immobilized β-glucanase shows sustained enzyme activity,good ethanol tolerance,and was reusable for the preparation of C-K from SLPN. | DONG Hui-juan1,2,JIANG Bin-hui3,HAN Ying3,GENG Yong1,ZHAO Yu-qing4 1.Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China 2.Graduate School of Chinese Academy of Sciences,Beijing 100039,China 3.School of Resources and Civil Engineering,Northeastern University,Shenyang 110004,China 4.Shenyang Pharmaceutical University,Shenyang 110016,China | 2010 | Chinese Herbal Medicines2010，2，1： | 20 |
| 7 | Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models显示文摘The traditional Chinese medicine concepts of 'Xinxueyuzuzheng (heart blood stasis obstruction pattern)' and 'Qiyinliangxuzheng (qi and yin deficiency pattern)' for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two 'pattern' rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two 'patterns' are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two 'Zhengxing (pattern types)' of myocardial ischemia, and 'Bianzheng (pattern identification)'. | YAN Bei1, A JiYe1, HAO HaiPing1, WANG GuangJi1, ZHU XuanXuan2, 3, ZHA WeiBin1, LIU LinSheng1, GUAN EnZe3, ZHANG Ying1, GU ShengHua1, HUANG Qing1 & ZHENG YuanTing1 1Key Laboratory of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, China 2Pharmacological Laboratory of Clinical Research Institute, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 210029, China 3College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China | 2009 | Science China(Life Sciences)2009，52，11： | 19 |
| 8 | Induction of apoptosis by furanodiene in HL60 leukemia cells through activation of TNFR1 signaling pathway显示文摘Objective Curcuma wenyujin,named Ezhu in Chinese,a traditional Chinese medicine,which has been shown to possess anticarcinogenic activity and used for the treatment of tumor in China,for example,hepatic cancer and leukemia.β-elemene,a component of Curcuma wenyujin,was largely reported as a main active component for anti-tumor effect of Curcuma wenyujin.However,furanodiene,another main component of Curcuma wenyujin,was seldom investigated for its anti-tumor activities.In the present study,we aim to investigate the effect of furanodiene on human leukemia HL60 cells and to study the accurate molecular mechanisms of its action.Methods Trypan blue exclusion experiment was used for cell growth inhibition assay.The apoptotic characterizations were assessed by flow cytometry analysis,AO/EB staining assay and agarose gel electrophoresis assay.The expression of apoptosis-related proteins and the release of cytochrome c from mitochondrial were detected by western blotting,and the mRNA levels of TNF-α and TNFR1 were probed by RT-PCR.Formation of TNFR1 complex was analyzed by using immunoprecipitation of TNFR1 with TRRF1/2 and RIP.Results Furanodiene(10-100 μM)treatment inhibited the growth of HL60 cells in a concentration-dependent manner.The effect of furanodiene on HL60 cells was associated with the induction of apoptosis,which was characterized by DNA fragmentation,cleavage of poly(ADP-ribose)polymerase(PARP),caspase-3,caspase-8 and caspase-9.In the Bcl-2 family proteins,Bid protein(a substrate of caspase-8)was activated by furanodiene,but Bcl-2,Bax and Bcl-xL proteins were not inuenced by furanodiene stimulation.Furanodiene elicited cytochrome c release from mitochondria into the cytosol.Moreover,furanodiene treatment caused the upregulation of TNFR1,the formation of TNFR1 complex and an obvious production of TNF-α in HL60 cells.The soluble TNFR1 receptor effectively inhibited furanodiene-induced apoptosis.Conclusions Furanodiene inhibited the growth of HL60 leukemia cells via induction of apoptosis.Furanodiene-induced apoptosis in HL60 cells is mediated by upregulation of TNF receptor 1 as well as induction of TNF-α production to activate TNFR1 signaling pathway.Our research provides insight into the molecular mechanisms on furanodiene-induced cell death,and may aid to the development of furanodiene as a new anti-tumor agent. | MA En-long1,WANG Xiao-long1,LI Yan-chun1,TAI Wen-jiao1,LI Te1,GUO Tao2(1.Department of Pharmacology,Shenyang Pharmaceutical University,Shenyang 110016,China 2.Department of Pharmacy,General Hospital of Shenyang Military Region,Shenyang 110016,China) | 2008 | 沈阳药科大学学报2008，25，S1： | 14 |
| 9 | Effects of Astragaloside Ⅳ Derivative on Heart Failure in Rats显示文摘Objective Astragaloside Ⅳ derivative(ASId) is one of Astragaloside Ⅳ(ASI) derivatives with higher water-solubility and may have more druggability than ASI.The present study aims at observing the effects of ASId on cardiovascular parameters in chronic heart failure in rats.Methods Using echocardiographic and haemodynamic measurements,the effects of ASId on congestive heart failure(CHF) induced by ligation of the left coronary artery in rats were investigated.Results ASId iv 0.5,1.0,and 2.0 mg/(kg·d) attenuated the decline of ejection fraction.The peak derivatives of the left ventricle(LV) pressure(dp/dt) in ASId treated groups were significantly increased.Both LV volumes in diastole and in systole were decreased significantly after ASId treatment,accompanied with a trend towards normalization of relative wall thickness at end-systole.ASId 0.5,1.0,and 2.0 mg/(kg·d) attenuated the increase of LV systolic and diastolic wall stress.ASId treatment also inhibited compensatory hypertrophy of depressed heart.Conclusion ASId could improve cardiac functions and inhibite compensatory hypertrophy and LV remodelling,which suggests the possibility of ASId as a new therapeutic drug for the treatment of CHF. | WANG Wei-ting1,ZHAO Zhuan-you1,HAN Ying-mei2,XU Wei-ren2,TANG Li-da1 1.State Key Laboratory of Pharmacokinetics and Pharmacodynamics,Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China 2.Tianjin Key Laboratory of Molecular Design & Drug Discovery,Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China | 2010 | Chinese Herbal Medicines2010，2，1： | 13 |
| 10 | A New Bibenzyl Derivative from Dendrobium moniliforme显示文摘A new bibenzyl derivative, 3,4-dihydroxy-4,5-dimethoxy bibenzyl, was isolated from a orchid Dendrobium moniliforme. The structure elucidation and 1H,13C NMR assignments were achieved by spectroscopic method. | Zhi Ming BI, Li YANG, Zheng Tao WANG*, Luo Shan XU, Guo Jun XU Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038 | 2002 | Chinese Chemical Letters2002，13，6： | 12 |
| 11 | Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography显示文摘Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production.Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract of A.vera.The biphasic solvent system composed of hexane-ethyl acetate-acetone-water(0.2:5:1.5:5) was used at a flow rate of 1.0 mL/min,while the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min.The effluent was detected at 254 nm.Results Isoaloeresin D(53.1 mg) and aloin(106.9 mg) were separated from the crude extract(384.7 mg) with the purities of 98.6% and 99.5%,respectively.Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe. | WAN Jin-zhi1,CHEN Xin-xia1,QIU Chun-mei1,LIN Xiang-cheng2 1.Laboratory for Pharmaceutical Analysis and Quality Evaluation,School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China 2.Institute of Fine Chemicals and Synthetic Drugs,Sun Yat-sen University,Guangzhou 510006,China | 2010 | Chinese Herbal Medicines2010，2，2： | 12 |
| 12 | CHEMICAL CONSTITUENTS OF LONICERA MACRANTHOIDES HAND.-MAZZ Ⅰ.STRUCTURE OF MACRANTHOISIDE I显示文摘A new bisdesmosidic triterpene saponin,macranthoiside I,wasisolated from L.macranthoides.Its structure was determinedby chemical degradation and spectroscopic analysis. | Min CHEN Si Qi LUO Hui Ting LI Shanghai Institute of Pharmaceutical Industry Shanghai,200040 | 1990 | Chinese Chemical Letters1990，1，3： | 11 |
| 13 | Investigation of the differences between the “COLD” and “HOT” nature of Coptis chinensis Franch and its processed materials based on animal’s temperature tropism显示文摘The description and differentiation of the so-called 'Cold' and 'Hot' natures, the primary 'Drug Naure' of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the 'Cold' and the 'Hot' natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40℃ ) significantly increased (P<0.05), suggesting the enhancement of Hot tropism. The internal indexes of adenosine triphosphatase (ATPase) activity and oxygen consuming volume decreased significantly (P<0.05), suggesting the decapability of energy metabolism. This external behavior of Hot tropism might reflect the internal Cold nature of C. chinensis Franch. However, the processed materials of C. chinensis Franch exhibited a different Cold nature in temperature tropism compared with crude C. chinensis Franch (CC): the Cold nature of bile-processed C. chinensis Franch (BC) enhanced while the ginger-processed C. chinensis Franch (GC) changed inversely. The changing sequence was consistent with the theoretical prognostication. It is indicated that the external Cold & Hot natures of Chinese medicine may possibly reflect in an ethological way for the changes of animal’s temperature tropism which might be internally regulated by the body’s energy metabolism. | ZHOU CanPing1,2, WANG JiaBo1, ZHANG XueRu1,2, ZHAO YanLing1, XIA XinHua2, ZHAO HaiPing1, REN YongShen1 & XIAO XiaoHe1 1 China Military Institute of Chinese Meteria Medica, 302 Military Hospital of China, Beijing 100039, China 2 College of Pharmaceutical Sciences, Hunan University of Chinese Medicine, Changsha 410208, China | 2009 | Science China(Life Sciences)2009，52，11： | 11 |
| 14 | 医改进行时——我国医院药学发展专家共识显示文摘新一轮医改使医院药学的发展面临机遇和挑战,给我国的医院药师提出了新的更高的要求。在中国药学会医院药学专业委员会等7个医院药师学术团体的牵头下,全国医院药学专家通过热烈的讨论达成本共识,以期能够促进医院药学的发展、促进人民群众的合理用药、提高我国药师的职业素养和职业地位、构建和谐与可持续发展的医疗环境。 | Hospital Pharmacy Committee of Chinese Pharmaceutical Association | 2014 | 药品评价2014，11，12： | 11 |
| 15 | Development of HPLC Method to Evaluate Drug-processing Technique of Eucommiae Cortex显示文摘Objective To establish a RP-HPLC method investigate the processing technique and mechanism of Eucommiae Cortex.Methods The RP-HPLC method was applied to simultaneously determining six ingredients,geniposidic acid,geniposide,genipin,chlorogenic acid,(+)-pinoresinol-di-β-D-glucopyranoside,and(+)-syringaresinol-di-β-D-glucopyranoside,in the different processed barks of Eucommia ulmoides.Results The valid method with good accuracy could be well used to study the processing technique of E.ulmoides;Besides,target ingredients in E.ulmoide were decreased within 6 h when they were processed.Conclusion Established RP-HPLC is a reliable method which could be used to research the processing technique of the barks of E.ulmoides.Moreover,the result of this study could be provided with significant evidence of processed barks of E.ulmoides. | WANG Jia-long1,LIU Er-wei1,2,WU Shuai1,YIN Zhao-ye3,ZHANG Yi2 1.Institute of Traditional Chinese Medicine Research,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 2.Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China 3.School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China | 2011 | Chinese Herbal Medicines2011，3，3： | 10 |
| 16 | FOXP3 expression and clinical characteristics of hepatocellular carcinoma显示文摘AIM: To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC). METHODS: We analyzed the expression and localization of FOXP3 in HCC tissues and cell lines to evaluate its biological features. The relationship between FOXP3 staining and clinical risk factors of HCC was assessedto identify the clinical characteristics of FOXP3 in HCC. RESULTS: The mRNA and protein expression of FOXP3 were found in some hepatoma cell lines. Immunohistochemical (IHC) analysis of HCC sections revealed that 48% of HCC displayed FOXP3 staining, but we did not find any FOXP3 staining in normal liver tissues and para-tumor tissues. IHC and Confocal analysis showed that the expressions of FOXP3 were mainly present in the nucleus and cytoplasm of tumor cells in tissues or cell lines. In HCC, the distribution of FOXP3 was similar to that of the cirrhosis, but not to the hepatitis B virus. Those findings implicate that FOXP3 staining seems to be associated with the high risk of HCC. CONCLUSION: The clinical characteristics of FOXP3 in HCC warrants further studies to explore its functions and roles in the cirrhosis and development of HCC. | Wei-Hua Wang, Jun-Zhi Wang, National Center for Safety Evaluation of Drugs, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China Wei-Hua Wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, Biotechnology Center, School of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Wei-Hua Wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, State Key Laboratory of Cancer Biology, Xi’an 710032, Shaanxi Province, China Wei Yan, Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Yu-Hai Zhang, Department of Health Statistics, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Jiang-Tao Yang, Department of Pathology, Xi’an Gaoxin Hospital, Xi’an 710032, Shaanxi Province, China | 2010 | World Journal of Gastroenterology2010，16，43： | 10 |
| 17 | Studies on Absorption and Tansport of Limoninoids from Fructus Evodiae in Caco-2 Cell Monolayer Model显示文摘Objective To study the intestinal absorption and transepithelial transport of three limoninoids: evodol (EVO), limonin (LIM), and shihulimonin A (SHIA), isolated from Fructus Evodiae [the unripe fruit of Evodia rutaecarpa and Evodia rutaecarpa var. bodinieri] in the human intestine. Methods The in vitro cultured human colon carcinoma cell line, Caco-2 cell monolayer model, was applied to studying the absorption and transepithelial transport of the three limoninoids from apical (AP) to basolateral (BL) side and from BL to AP side. The three limoninoids were measured by reversed-phase high performance liquid chromatography coupled with ultraviolet absorption detector. Transport parameters and apparent permeability coefficients (Papp) were then calculated and compared with those of Propranolol as a control substance of high permeability and Atenolol as a control substance of poor permeability. Results The Papp value of EVO and LIM from AP to BL side for absorption and transport were 1.78 × 10-5 cm/s and 1.16 × 10-5 cm/s, respectively, which was comparable to that of Propranolol with Papp 2.18 × 10-5 cm/s. Conclusion The absorption and transport of both EVO and LIM are main passive diffusion as the dominating process in Caco-2 cell monolayer model, and they were estimated to be high absorbed compounds. SHIA in Caco-2 cell monolayer model may be involved in metabolism in the transport processes. | YANG Xiu-wei1*, TENG Jie1, ZHAO Bo2, ZHANG Lian-xue2 1. State Key Laboratory of Natural and Biomimetic Drugs (Peking University), School of Pharmaceutical Sciences, Peking University, Beijing 100191, China 2. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China | 2009 | Chinese Herbal Medicines2009，1，1： | 10 |
| 18 | A New Phenolic Glucoside from Paeonia lactiflora显示文摘Objective To study the chemical constituents from EtOAc extracts of Paeonia lactiflora.Methods Compounds were isolated by various chromatographic techniques and structures were elucidated on the basis of spectral analysis.Results Seventeen compounds were obtained and their structures were identified as 1,2,6-benzenetriol-1-O-α-D-glucoside(1),paeoniflorin(2),4-methylpaeoniflorin(3),albiflorin(4),paeonidanin(5),benzoylpaeoniflorin(6),4-methylbenzoylpaeoniflorin(7),benzoylalbiflorin(8),paeonidanin A(9),galloylalbiflorin(10),debenzoylalbiflorin(11),4',5-dihydroxyflavanone-7-O-β-D-glucoside(12),5,7-dihydroxy flavanone-4'-O-β-D-glucoside(13),(+)-catechin(14),gallic acid(15),vanillic acid(16),and 1,2,3-benzenetriol(17).Conclusion Compound 1 is a new compound named paeoniphenoside.Compounds 12 and 13 are firstly obtained from genus Paeonia L.,and compounds 5 and 9 are isolated from P.lactiflora for the first time. | HE Xiao-yan,HAN Li,HUANG Xue-shi Department of Natural Products Chemistry,School of Pharmaceutical Science,China Medical University,Shenyang 110001,China | 2011 | Chinese Herbal Medicines2011，3，2： | 9 |
| 19 | Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales,Phaeophyta)显示文摘The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been inte-grated into the genome of gametophytes of L. japonica, and the expression product showed the ex-pected bioactivity, implying the proper post-transcript modification in haploid gametophyte. | ZHANG YiChen1,2, JIANG Peng1, GAO JiangTao1,3, LIAO JianMin4, SUN ShiJing4, SHEN ZiLong4 & QIN Song1, 1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 2 College of Chemistry and Life Science, Tianjin Normal University, Tianjin 300387, China 3 Graduate University of Chinese Academy of Sciences, Beijing 100049, China 4 Biotechnology Center, China Pharmaceutical University, Nanjing 210009, China | 2008 | Science China(Life Sciences)2008，51，12： | 9 |
| 20 | Cloning of cytochrome P-450 2C9 cDNA from human liver and its expression in CHL cells显示文摘AIM: Using bacterial, yeast, or mammalian cell expressing a human drug metabolism enzyme would seem good way to study drug metabolism-related problems. Human cytochrome P-450 2C9 ( CYP2 C9) is a polymorphic enzyme responsible for the metabolism of a large number of clinically important drugs. It ranks among the most important drug metabolizing enzymes in humans. In order to provide a sufficient amount of the enzyme for drug metabolic research, the CYP2 C9 eDNA was cloned and expressed stably in CHL cellsMETHODS: After extraction of total RNA from human livertissue, the human CYP2C9 eDNA was amplified withreverse transcription-polymerase chain reaction (RT-PCR),and cloned into cloning vector pGEM-T. The cDNA fragmentwas identified by DNA sequencing and subcloned into amammalian expression vector pREP9. A transgenic cell linewas established by transfecting the recombinant vector ofpREP9-CYP2C9 into CHL cells. The enzyme activity ofCYP2C9 catalyzing oxidation of tolbutamide to hydroxytolbutamide in S9 fraction of the cell was determined by highperformance liquid chromatography(HPLC).RESULTS: The amino acid sequence predicted from theeDNA segment was identical to that of CYP2 C9 * 1, the wildtype CYP2 C9. However, there were two base differences, i.e. 21T > C, 1146C > T, but the encoding amino acidsequence was the same, L7, P382. The S9 fraction of theestablished cell line metabolizes tolbutamide to hydroxytolbutamide; tolbutamide hydroxylass activity was found to be0.465 ± 0.109 μmol@ min-1 . g1 S9 protein or 8.62 ± 2.02 mol@ min 1 ~mol-1 CYP, but was undetectable in parental CHL cell.CONCLUSION: The cDNA of human CYP2C9 was successfullycloned and a cell line of CHL- CYP2C9, efficiently expressingthe protein of CYP2C9, was established. | Ge-Jian Zhu Ying-Nian Yu,Department of Pathophysiology and Laboratory of Medical Molecular Biology,Zhejiang University School of Medicine,Hangzhou 310031,Zhejiang Province,China Xin Li,Department of pharmaceutical analysis & drug metabolism,College of Pharmacology Science,Zhejiang University,Hangzhou 310031,Zhejiang Province,China Yu-Li Qian, Present address:Center of laboratory,Women’s hospital,School of Medicine,Zhejiang University,Hangzhou 310031,Zhejiang Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 9 |
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