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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Rapid method for estimating leaf area per plant in maize显示文摘 | Pearce R B Mocd J J Bailey T B | 1975 | Crop Science1975，5，15： | 2 |
| 3 | A study of selerochronology by laser ablation ICP-MS 显示文摘 | Toland H Perkins B Pearce N | 2000 | Journal of Analytical Atomi Spectrometry2000，15，： | 1 |
| 4 | Characterization of programmed cell death during aerenchyma formation induced by ethylene of hypoxia in roots of maize (Zea mays L)显示文摘 | GUNAWARDENA A PEARCE D M JACKSON M B etal | 2001 | Planta2001，212，1： | 1 |
| 5 | Activation tagging of a dominant gibberellin catabolism gene (GA 2-oxidase) from poplar that regulates tree stature显示文摘 | BUSOV V B MEILAN R PEARCE D W | 2003 | Plant Physiol2003，132，3： | 1 |
| 6 | Numerical circulation model for wind-induced flow显示文摘 | Cooper C K | 1981 | J Hydr Div ASCE1981，107，3： | 1 |
| 7 | Management of hypothyroidism in adults显示文摘 | Vaidya B Pearce SH | 2008 | BMJ2008，337，： | 1 |
| 8 | Trace element discrimination diagrams for the tectonic interpretation of granitic rocks 显示文摘 | Pearce J A Harris N B W Tindle A G | 1984 | J Petrol1984，25，4： | 1 |
| 9 | Trace element discrimination diagrams for the tecotonic interpretation of granitic rocks显示文摘 | Pearce J A Harris N B W and Tindle A G | 1984 | Journal of Petrology1984，25，： | 1 |
| 10 | Rubber toughening of practical tetragly-cidyl methylenedianiline-piperidine adduct systems显示文摘 | Pearce P J Morris C E M Ennis B C | | 0，，： | 1 |
| 11 | The proinflammatory cytokine,interleukin-1alpha,reduces glucocorticoid receptoR translocation and function显示文摘 | Pariante C M Pearce B D Pisell T L | 1999 | Endocrinology1999，140，9： | 1 |
| 12 | Trace element dis- crimination diagrams for the tectonic interpretation of granitic rocks显示文摘 | Pearce J A Harris N B W Tindle A G | 1984 | Journal of Petrology1984，25，4： | 1 |
| 13 | Trace element discri- mination diagrams for the tectonic interpretation of granitic rocks 显示文摘 | Pearce J A Harris N B W Tindle A G | 1984 | Journal of Petrology1984，25，： | 1 |
| 14 | Degeneration and the chemical composition of the human lumbar intervertebral disc显示文摘 | PEARCE R H GRIMMER B J ADAMS M E | 1987 | J Orthop Res1987，5，2： | 1 |
| 15 | Trace element discrimi- nation diagrams for the tectonic interpretation of granitic rocks 显示文摘 | Pearce J A Harris N B W Tindle A G | 1984 | Journal of Petrology1984，25，： | 1 |
| 16 | Antibiotic use in infancy and symptoms of asthma,rhinoconjunctivitis,and eczema in children 6 and 7 years old:International Study of Asthma and Allergies in Childhood Phase III显示文摘 | Foliaki S Pearce N Bjrkstén B | | 0，，05： | 1 |
| 17 | Glycinebetaine and choline in wheat;distribution and relation to infection by Fusarium graminearum显示文摘 | Pearce R B Strange R N Smith H | 1976 | Phytochem-istry1976，15，： | 1 |
| 18 | The role of mat-forming diatoms in the formation of Mediterranean sapropels 显示文摘 | Kump A E S Pearce R B Koizumi I | 1999 | Nature1999，398，： | 1 |
| 19 | Trace Element Discrimination Diagrams for the Tectonic Interpreta- tion of Granitic Rocks显示文摘 | Pearce J A Harris N B W Tindle A G | 1984 | Journal of Petrology1984，25，： | 1 |
| 20 | Trace element discrimination diagrams for the tectonic interpretation of granitic rocks显示文摘 | Pearce J A Harris N B W Tindle A G | 1984 | J Petrol1984，25，4： | 1 |
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