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| 1 | Highly Pathogenic H5N1 Influenza Virus Infection in Migratory Birds显示文摘 | Jinhua Liu et al.College of Veterinary Medicine, China Agricultural University, Beijing 100094, China. | 2005 | 中国实验动物学报2005，13，S1： | 114 |
| 2 | Increasing the frequency of CIK cells adoptive immunotherapy may decrease risk of death in gastric cancer patients显示文摘AIM: To analyze the correlation between cytokineinduced killer (CIK) cells adoptive immunotherapy and cancer-related death in gastric cancer patients. METHODS: One hundred and fifty-six gastric cancer patients after operation at the Third Affiliated Hospital of Soochow University were enrolled in this study. Their clinical data including demographic characteristics, operation time, tumor size, pathological type and staging, tumor metastasis, outcome of chemotherapy or CIK cells adoptive immunotherapy, survival time or time of death were collected with a standard structured questionnaire. Kaplan-Meier method was used to estimate the median survival time, and the 2- and 5- year survival rates. Hazard risk (HR) and 95% confidence interval (95% CI) of CIK cells adoptive immunotherapy for gastric cancer were calculated using the two-stage time-dependent covariates Cox model. RESULTS: The survival time of gastric cancer patients was longer after CIK cells adoptive immunotherapy than after chemotherapy (χ 2 = 10.907, P = 0.001). The median survival time of gastric cancer patients was also longer after CIK cells adoptive immunotherapy than after chemotherapy (49 mo vs 27 mo, P < 0.05). The 2- and 5-year survival rates of gastric cancer patients were significantly higher after CIK cells adoptive immunotherapy than after chemotherapy (73.5% vs 52.6%, 40.4% vs 23.9%, P < 0.05). A significant difference was observed in the survival curve for patients who received CIK cells adoptive immunotherapy (0, 1-10, 11-25, and over 25 frequencies) (χ 2 = 14.534, P = 0.002). The frequencies of CIK cells adoptive immunotherapy were significantly related with the decreasing risk of death in gastric cancer patients after adjustment for sex and age of the patients, tumor stage and relapse (HR = 0.54, 95% CI: 0.36-0.80) when the first stage Cox model was used to define the subjects who remained alive beyond 36 mo as survivors. However, no correlation was observed between the frequencies of death in CIK cells adoptive immunotherapy and the risk of gastric cancer patients (HR = 1.09, 95% CI: 0.63-0.89) when the second stage Cox model was used to define the subjects who survived for more than 36 mo as survivors. CONCLUSION: The survival time of the gastric cancer patients treated with chemotherapy combined with CIK cells adoptive immunotherapy is significantly longer than that of the patients treated with chemotherapy alone and increasing the frequency of CIK cells adoptive immunotherapy seems to benefit patients more. | Jing-Ting Jiang, Chang-Ping Wu, Lu-Jun Chen, Xiao Zheng, Department of Tumor Biological Treatment, Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu Province, China Yi-Bei Zhu, Jing Sun, Xue-Guang Zhang, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, Soochow University, Suzhou 215123, Jiangsu Province, China Yue-Ping Shen, Wen-Xiang Wei, Department of Medicine, Soochow University, Suzhou 215123, Jiangsu Province, China Bin-Feng Lu, Department of Immunology, University of Pitts- burgh School of Medicine, Pittsburgh, PA 15261, United States | 2010 | World Journal of Gastroenterology2010，16，48： | 82 |
| 3 | 肉毒毒素治疗成人肢体痉挛状态 中国指南(2015)显示文摘本指南是在参考《英国国家指南:肉毒毒素治疗成人痉挛状态》、《美国评价:肉毒毒素治疗痉挛状态的循证回顾》和《A型肉毒毒素治疗成人痉挛状态的欧洲共识》等文献的基础上,综合我国康复医学专家的共同意见而形成的。◆痉挛状态(spasticity)为目前较容易被各方面接受的泛指名词,定义是由上运动神经元损害之后的运动感觉控制障碍导致的,各种间歇或持续的非自主的肌肉活动。痉挛状态包括牵张反射亢进、协同运动、联合反应、屈肌反射增强和痉挛性肌张力障碍等上运动神经元综合征阳性征在内的异常肌肉活动。痉挛状态可导致各种问题,如疼痛、压疮、主动/被动活动受损、护理困难、外观受损,并进而影响活动和参与。痉挛状态的处理比较复杂。◆局部肌肉注射肉毒毒素(botulinum neurotoxin,Bo NT)是治疗痉挛状态的良好方法,疗效确切,不良反应轻微。有大量循证Ⅰ级证据说明,这种方法能降低过高的肌张力,并改善患者功能。◆市场上有不同的Bo NT产品,不同Bo NT产品的生产工艺、配方、结构及分子大小都不同,且采用不同的生物鉴定方法,故不同的Bo NT产品的剂量单位不可相互换算。不同的Bo NT产品的疗效可能存在差异,不良事件的发生率可能不同。目前的临床证据显示:保妥适安全性良好,其引起的不良事件的发生与安慰剂无显著差异。近年来经改良的保妥适配方,不易引起免疫反应。◆本指南目的是给专科医生提供参考,以便于合理使用Bo NT治疗痉挛状态。◆因临床研究的滞后,Bo NT在各国的适应证不尽相同。大多数国家包括中国的专家均认为:有经验的临床医生可以使用Bo NT合理治疗痉挛状态。◆只有在痉挛状态评价和治疗方面有经验的临床医生,才能使用Bo NT。其主要作用是降低注射肌的张力,易化注射肌的牵伸和延长,并有助于增加拮抗肌肌力。然而,只有配合物理/作业/矫形器等其他治疗,才能使上运动神经元综合征患者的功能发生更有意义的改善。因此,Bo NT不能单独使用,而是多学科综合疗法的组成部分。◆选择合适的患者,制订明确、切实可行并且可测评的目标,对用Bo NT治疗痉挛状态取得成效至关紧要。治疗目标或为改善症状,或为改善主动功能,或为改善肢体的活动范围和减轻护理工作量。这些目标建议应记录于患者的病历中。每次注射Bo NT时,都应评价治疗效果。 | Chinese Association of Rehabilitation Medicine | 2015 | 中国康复医学杂志2015，30，1： | 82 |
| 4 | 肿瘤相关抑郁状态中医诊疗专家共识显示文摘《肿瘤相关抑郁状态中医诊疗专家共识》由6部分组成:1定义:肿瘤相关抑郁是以肿瘤为基础疾病而引发的一组症状或状态,并非精神病性抑郁,推荐用'肿瘤相关抑郁状态'名词术语;建议用'郁证'作为中医病证名。2发病:心理因素、癌性疼痛、癌性疲乏、睡眠障碍、手术创伤、放疗、化疗与肿瘤相关抑郁状态发生密切相关;因郁致病、因病致郁以及肝郁、脾虚导致痰、湿、瘀相互交织是中医关键病因病机。3症状:由核心症状、心理症状与躯体症状三部分构成。其中,心境或情绪低落、兴趣缺乏与乐趣丧失是临床诊断的主要依据。4筛查:参考国际神经精神访谈量表,结合数字疼痛分级法制定调查问卷,对肿瘤患者进行抑郁状态筛查是为了区分精神病性抑郁症与肿瘤相关抑郁,以帮助肿瘤科医生能够迅速甄别、及时治疗或转诊。5诊断:参照中国精神障碍分类与诊断标准(CCMD-3),结合肿瘤患者诊治过程的心境变化来综合考虑。6治疗:必须与抗肿瘤同时进行。轻度抑郁状态可采用心理干预、音乐放松、教育引导、体育训练、针灸等非药物干预的综合疗法;中度以上抑郁状态,以中医辨证治疗为主,辅助抗抑郁药物治疗;严重抑郁状态或发展或/并构成精神疾病诊断时,应按照中国法律,及时转诊到精神病医院或转交精神科医生诊治。 | Hematology Branch of Chinese Association of Chinese Medicine | 2015 | 中华中医药杂志2015，30，12： | 83 |
| 5 | Clinical observation of salvianolic acid B in treatment of liver fibrosis in chronic hepatitis B显示文摘AIM: To evaluate the clinical efficacy of salvianolic acidB (SA-B) on liver fibrosis in chronic hepatitis B.METHODS: Sixty patients with definite diagnosis of liverfibrosis with hepatitis B were included in the trial.Interferon-γ (IFN-γ) was used as control drug. Thepatients took orally SA-B tablets or received muscularinjection of IFN-γ in the double blind randomized test,The complete course lasted 6 months. The histologicalchanges of liver biopsy specimen before and after thetreatment were the main evidence in evaluation, incombination with the results of contents of serum HA,LN, Ⅳ-C, P-Ⅲ-P, liver ultrasound imaging, andsymptoms and signs.RESULTS: Reverse rate of fibrotic stage was 36.67 % inSA-B group and 30.0 % in IFN-γgroup. Inflammatoryalleviating rate was 40.0 % in SA-B group and 36.67 %in IFN-γ group. The average content of HA and Ⅳ-Cwas significantly lower than that before treatment. Theabnormal rate also decreased remarkably. Overallanalysis of 4 serological fibrotic markers showedsignificant improvement in SA-B group as comparedwith the IFN-γgroup. Score of liver ultrasound imagingwas lower in SA-B group than in IFN-γgroup (HA 36.7 %vs80 %,Ⅳ-C 3.3 % vs23.2 %). Before the treatment,ALT AST activity and total bilirubin content of patientswho had regression of fibrosis after oral administrationof SA-B, were significantly lower than those of patientswho had aggravation of fibrosis after oraladministration of SA-B. IFN-γ showed certain sideeffects (fever and transient decrease of leukocytes,occurrence rates were 50 % and 3.23 %), but SA-Bshowed no side effects.CONCLUSION: SA-B could effectively reverse liverfibrosis in chronic hepatitis B. SA-B was better than IFN-γ in reduction of serum HA content, overall decrease of4 serum fibrotic markers, and decrease of ultrasoundimaging score. Liver fibrosis in chronic hepatitis B withslight liver injury was more suitable to SA-B in anti-fibrotic treatment. SA-B showed no obvious side effects. | Ping Liu Yi-Yang Hu Cheng Liu Hui-Ming Xue Zhi-Qiang Xu Lie-Ming Xu Cheng-Hai Liu Hong-Tu Gu Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China Da-Yuan Zhu Shanghai Institute of Metaria Medica,Chinese Academy of Sciences,Shanghai 200031,China Zhi-Qing Zhang the 4~(th) Hualyin City Hospital,Hualan City,223000,JiangSu Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 85 |
| 6 | 肉毒毒素治疗成人肢体肌痉挛中国指南(2010)显示文摘本文在参考《A型肉毒毒素治疗成人痉挛的欧洲共识》和《肉毒毒素治疗痉挛英国全国指南》的基础上,尊重我国康复专家的共同意见形成的指南。肌痉挛是肌肉不自主的运动过度,通常发生于中枢神经系统(脑和脊髓)损伤以后。痉挛表现多种多样,具体取决于病灶的大小、部位和损伤时间。肌痉挛可导致各种有害的结果,如疼痛、畸形和功能受损。肌痉挛的处理比较复杂。局部肌肉注射肉毒毒素(botulinum toxin,BT)是治疗局灶性肌痉挛普遍接受方法,耐受性很好。有大量强有力的I级证据说明了这种方法能降低痉挛患者的肌肉张力,继而改善患者功能。市场上有不同的肉毒毒素产品,不同肉毒毒素产品的生产工艺、配方、结构及均匀程度都不同,且采用不同的生物鉴定方法,故不同肉毒毒素产品剂量单位不可互换。不同肉毒毒素产品治疗肌痉挛都有效,但其耐受性和不良事件发生率不同。目前的临床证据显示:保妥适安全性良好,剂量增加不会导致副反应的增加;1997年以后的保妥适配方,纯度增加,抗体形成是很罕见的事件(1/191例成年人),不再是一个有临床意义的问题。该指南主要针对康复医学科,目的是给康复医生提供参考,以便他们能合理使用肉毒毒素治疗痉挛;但也可以作为其他学科应用的参考。肉毒毒素在各国的适应证不尽相同,大多数国家包括我国的专家均认为:有经验的临床医生可以超出说明书范畴而用于其他肌群的注射。只有在肌痉挛的评价和治疗方面有经验的临床医生才能注射肉毒毒素。肌痉挛的治疗主要目标是让肌肉伸展,恢复正常位置。因此,肉毒毒素不能单独使用,而是多学科综合疗法的组成部分。选择合适的患者以及制定明确、切实可行并且可测评的目标,对用肉毒毒素治疗肌痉挛取得成效至关紧要。采取各种干预措施的共同目标都是为了缓解疼痛、改善肢体的活动范围、减轻护理负担,某些情况下可以增强主动功能或为恢复主动功能提供机会。这些目标建议应记录于患者的病历中,每次注射肉毒毒素时,都应评价治疗效果。治疗效果的评价指标应与所记录的治疗目标相符。按照该指南使用肉毒毒素,有可能预防挛缩和畸形,从而减轻患者和医护人员的负担。 | Chinese Association Of Rehabilitation Medicine | 2010 | 中国康复医学杂志2010，25，6： | 63 |
| 7 | Pharmacopoeia of the People's Republic of China(2010 Edition):A Milestone in Development of China's Healthcare显示文摘Compilation of the Pharmacopoeia of the People's Republic of China (known as the Chinese Pharmacopoeia 2010) has been completed by the Ninth Chinese Pharmacopoeia Commission, which has been officially distributed in January 2010 and implemented on October 1st, 2010. | QIAN Zhong-zhi1,DAN Yang2,LIU Yan-ze2,PENG Yong2 1.Chinese Pharmacopoeia Commission,Beijing 100061,China 2.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China | 2010 | Chinese Herbal Medicines2010，2，2： | 55 |
| 8 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 9 | Roux-en-Y choledochojejunostomy using novel magnetic compressive anastomats in canine model of obstructive jaundice显示文摘BACKGROUND:The traditional hand-sewn Roux-en-Y choledochojejunostomy is technically complicated,and the incidence of postoperative complications has remained high.A set of novel magnetic compressive anastomats was introduced to facilitate choledochojejunostomy and improve the prognosis of patients.METHODS:After ligating the common bile duct for 7 days,16 dogs were randomly divided into two groups (n=8 per group).Anastomats were used in the study group,and the traditional hand-sewn method was used in the control group for standard Roux-en-Y choledochojejunostomy.We compared the operation time,incidence of complications,gross appearance,and pathological disparity in stoma between the two groups in 1-month and 3-month follow-up examinations.RESULTS:The time spent on constructing the anastomosis for the study group was significantly shortened.Although no anastomotic stenosis occurred in the two groups,the narrowing rate of biliary-enteric anastomosis was much higher in the control group.There was one case of bile leakage in the control group,whereas no bile leakage occurred in the study group.A smoother surface,an improved layer apposition,and a lower local inflammatory response were identified in the anastomosis of the study group.CONCLUSION:The structures of the novel magnetic compressive anastomats are simple,and they are time-saving,safe and efficient for performing Roux-en-Y choledocho- jejunostomy procedures in a canine model of obstructive jaundice. | Chao Fan,Xiao-Peng Yan,Shi-Qi Liu,Chun-Bao Wang,Jian-Hui Li,Liang Yu,Zheng Wu and Yi Lv Department of Hepatobiliary Surgery and Department of Pathology,First Affiliated Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an 710061,China Department of Surgical Oncology,Third Affiliated Hospital,School of Medicine,Xi’an Jiaotong University,Xi’an 710068,China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，1： | 49 |
| 10 | Effects of Chinese Jianpi herbs on cell apoptosis and related gene expression in human gastric cancer grafted onto nude mice显示文摘AIM: To explore the mechanism of the Sijunzi decoction and another Chinese herbal recipe (SRRS) based mainly on the Sijunzi decoction in treatment of gastric cancer.METHODS: A human gastric adenocarcinoma cell line SGC7901 grafted onto nude mouse was used as the animal model. The mice were divided into 3 groups, one control and the two representative experimental conditions. Ahimals in the two experimental groups received either Sijunzi decoction or SRRS over a 40-day period starting at 1st day after grafting. Control animals received saline on an identical schedule. Animals were killed 41 days after being grafted.The effect of therapy was assessed by two ways: (1)tumor size was periodically measured during the life of the animals; (2) tumor weight was determined by a electron balance immediately after the animals killed. For detection of apoptotic cells, apoptotic indices(AI) were examined by the terminal deoxynucleotidyl transferase-mediated deoxyuddine tdphosphate fluorescence nick end labeling (TUNEL) method.Morphological alterations were observed with electron microscopy. S-P immunohistochemical method was used to detect the expression of Ki-67 in xenografts. Expression of bcl-2 and p53 was semiquantitatively detected using a reverse transcriptase-polymerase chain reaction (RT-PCR)technique.RESULTS: When compared with controls, tumor growth (size and weight) was significantly inhibited by treatment with the Sijunzi decoction (P<0.05) or SRRS (P<0.01). The tumor inhibitory rate in the Sijunzi decoction group was 34.33 % and SRRS group 46.53 %. AI of human gastric cancer xenografts in nude mice was significantly increased to 16.24±3.21% using TUNEL method and 11.38±6.46 % by FACScan in the Sijunzi decoction group compared with the controls (TUNEL: 2.63±1.03 %, P<0.01; FACScan: 7.15± 1.32 %, P<0.05). SRRS group was also found a significantly increased AI by using TUNEL method and flow cytometry analysis compared with the controls (TUNEL: 13.18±3.05 %,P<0.05; FACScan: 11.58±5.71% (P<0.05). Under electron microscope, cell shrinkage, nuclear chromatin condensation,formation of membrane blebs and apoptotic bodies were frequently observed in Sijunzi decoction group and SRRS group. The average labeling index (LI) for Ki-67 in SRRS group was significantly decreased to 8.43±2.22 % compared with the control group (10.37±t.91%) (P<0.05). The average labeling index for Ki-67 in sijunzi decoction group was 7.95±2.54 % which was lower than that of the control group, but showed no significance (P=0.07). The expression level of p53 mRNA was lower in both Sijunzi decoction group and SRRS group than that in control group (P<0.05; P<0.01). The expression of bd-2 mRNA was also decreased in SRRS group compared with the control (P<0.01).CONCLUSION: The inhibition of gastric cancer cell growth in vivo by Chinese Jianpi herbs and SRRS is related to induction of the cell apoptosis which may be involved in aberrant expression of p53 and bcl-2 | Ai-Guang Zhao Hai-Lei Zhao Jin-Kun Yang Lai-Di Tang Department of Oncology,Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China Xiao-Jie Jin Renji Hospital,Shanghai Second Medical University,Shanghai 200032,China | 2002 | World Journal of Gastroenterology2002，8，5： | 43 |
| 11 | Diagnostic value of platelet derived growth factor-BB,transforming growth factor-131,matrix metalloproteinase-1,and tissue inhibitor of matrix metalloproteinase-1 in serum and peripheral blood mononuclear cells for hepatic fibrosis显示文摘AIM: Noninvasive diagnosis of hepatic fibrosis has become the focus because of the limited biopsy, especially in the surveillance of treatment and in screening hepatic fibrosis. Recently, regulatory elements involved in liver fibrosis, such as platelet derived growth factor-BB (PDGF-BB), transforming growth factor-β1(TGF-β1), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), have been studied extensively. To determine whether these factors or enzymes could be used as the indices for the diagnosis of hepatic fibrosis, we investigated them by means of receiver operating characteristic (ROC) curve. METHODS: Serum samples from sixty patients with chronic viral hepatitis B and twenty healthy blood donors were assayed to determine the level of PDGF-BB, TGF-β1, MMP-1, and TIMP-1 with ELISA, and HA, PCIII, C-IV, and LNlevel with RIA. The message RNA (mRNA) expression of TIMP-1 and MMP-1 in peripheral blood mononuclear cells (PBMCs) was detected by RT-PCR and Northern blot hybridization. Liver biopsy was performed in all patients. The biopsy samples were histopatholocjically examined. The trial was double-blind controlled. RESULTS: The serum level of PDGF-BB, TIMP-1, the ratio of TIMP-1 and MMP-1 (TIMP-1/MMP-1), mRNA expression of TIMP-1 (TIMP-lmRNA), and the ratio of TIMP-lmRNA and MMP-lmRNA (TIMP-lmRNA/MMP-lmRNA) in patients was significantly higher than those in the healthy blood donors (t=2.514-11.435, P=0.000-0.016). The serum level of PDGF-BB, TIMP-1, TIMP-1/MMP-1, and TIMP-lmRNA was positively correlated with fibrosis stage and inflammation grade (r=0.239-0.565, P=0.000-0.033), while the serum level of MMP-1 was negatively correlated with fibrosis stage and inflammation grade, and TIMP-lmRNA/MMP-lmRNA was positively correlated with inflammation grade. Through the analysis by ROC curve, serum PDGF-BB was the most valuable marker, and its sensitivity was the highest among the nine indices. The markers with the highest specificity were TIMP-lmRNA and TIMP-lmRNA/MMP-lmRNA in PBMCs. The area under the curve (AUC) of PDGF-BB, TIMP-lmRNA, TIMP-lmRNA/MMP-lmRNA, TIMP-1/MMP-1, HA, PCIII, TIMP-1, C-IV, and LN was 0.985, 0.876, 0.792, 0.748, 0.728, 0.727, 0.726, 0.583, and 0.463, respectively. The sensitivity and the specificity in the parallel test was 99.0% and 95.0 % when serum PDGF-BB, TIMP-lmRNA and TIMP-lmRNA/MMP-lmRNA was detected simultaneously. CONCLUSION: Serum level of PDGF-BB, TIIVIP-lmRNA, TIMP-lmRNA/MMP-lmRNA in PBMCs, and serum level of TIMP-1 and TIMP-1/MMP-1 can be used as the indices for the diagnosis of hepatic fibrosis, but the former three are more useful. The combination of serum PDGF-BB, TIMP-lmRNA and TIMP-lmRNA/MMP-lmRNA in PBMCs is even more efficient in screening liver fibrosis. | Bin-Bin Zhang Wei-Min Cai Hong-Lei Weng Zhong-Rong Hu Jun Lu Min Zheng Rong-Hua Liu, Institute of Infectious Diseases, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China | 2003 | World Journal of Gastroenterology2003，9，11： | 48 |
| 12 | L-forms of H. pylori显示文摘AIM: To study the occurrence of L-forms of H. pyloriinfection in patients with peptic ulcers and its association with possible changes of cellular immune function in the patients.METHODS: Endoscopic biopsy specimens of gastric antrum and gastric corpus were taken from 228 patients with peptic ulcers and inoculated into Skirrow selective medium for H.pylorivegetative forms and special medium for H. pylori Lforms, followed by bacterial isolation and identification. And peripheral venous blood of the patients was taken to detect the percentage of CD3+, CD4+ and CD8+ with biotin-streptavidin (BSA) and the level of IL-2, IL-6 and IL-8 with ElISA.RESULTS: (1) The detection rates of H. pyloriL-forms and vegetative forms in the patients were 50.88 % (116/228)and 64.91 % (148/228) respectively, and the co-infection rate of H. pyloriL-forms and vegetative forms was 78.38 % (116/148). To be more exact, the detection rates of H. pylori L-forms in male and female patients were 57.04 % (77/135) and 41.94 % (39/93) respectively, and statistics found significant difference between them (P<0.05). Furthermore, the detection rates of H. pyloriL-forms in patients aged 14 years-, 30 years-, 40 years- and 50 years- were 31.91%(15/47), 42.86 % (24/56), 56.94 % (41/72) and 67.92 %(36/53) respectively, and there was significant difference between them (P<0.011). (2) The percentages of CD3+, CD4+,CD8+, the ratio of CD4+/CD8+, and the level of IL-2, IL-6,IL-8 in H. pylori-positive patients were (52.59±5.44) %,(35.51±5.74) %, (27.77±8.64) %, (1.56±0.51), (2.66±0.47)mg/L, (108.62±5.85) ng/L and (115.79±7.18) ng/Lrespectively, compared with those in H. pylori-negative patients, the percentages of CD3+, CD4+ and the ratio of CD4+/CD8+ decreased, but the level of IL-2, IL-6 increased, and the difference was significant (P<0.001-P<0.011).Moreover, the percentages of CD3+, CD4+, CD8+, the ratio of CD4+/CD8+, and the level of IL-2, IL-6, IL-8 in the patients with mixed infection of bothH. pyloriL-forms and vegetative forms were (51.69±5.28) %, (34.75±5.89) %, (27.15±7.45) %, (1.48±0.47), (2.16±0.38) mg/L, (119.45±5.44) ng/L and (123.64±6.24) ng/L respectively, compared with those in patients with simple infection of H. pylorivegetative forms,the percentage of CD4+, the ratio of CD4+/CD8+ and the level of IL-2 increased, but the level of IL-6 and IL-8decreased, statistical difference was found between them (P<0.001-P<0.05).CONCLUSION: L-forms variation often occurs in patients with peptic ulcers who are infected byH. pylori, which is commonly found in male patients and related to ages. The L-forms variation of H. pylori can be an important factor causing disorder of cellular immune function in the patients with peptic ulcers who are infected by H. pylori. | Ke-Xia Wang Chao-Pin Li Yu-Bao Cui Ye Tian Qing-Gui Yang School of Medicine, Anhui University of Science & Technology Huainan 232001, Anhui Province, China | 2003 | World Journal of Gastroenterology2003，9，3： | 47 |
| 13 | Changes of gut bacteria and immune parameters in liver transplant recipients显示文摘BACKGROUND: Liver transplantation is one of the most effective therapeutic options for patients with end-stage liver diseases, and gut microbiota is actively involved in potential infections in pretransplant and posttransplant patients. However, the diversity of gut microbiota and its relationship with the immune parameter of liver transplantation recipients are not well understood. METHODS: We collected fresh feces and blood samples from 190 participants in China from November 2004 to May 2008, including 28 healthy volunteers, 51 cirrhotic patients and 111 liver-transplanted patients. Six interesting gut bacteria, plasma endotoxin, serum cytokines (i.e., tumor necrosis factor alpha and interleukin-6) and fecal secretory IgA (SIgA) were investigated by real-time quantitative PCR, chromogenic limulus amoebocyte assay, sandwich-type enzyme-linked immunosorbent assay and radioimmunoassay, respectively. RESULTS: All Eubacteria, Bifidobacterium spp., Faecalibacterium prausnitzii and Lactobacillus spp. were significantly lower in the liver transplantation recipients while Enterobacteriaceae and Enterococcus spp. were significantly higher (P<0.05). Except for Enterococcus spp., other bacteria showed a tendency to restore to normal level along with the time after liver transplantation. Plasma endotoxin, interleukin-6 and fecal SIgA in cirrhotic patients increased significantly, but not in liver transplantation recipients. Plasma endotoxin and interleukin-6 were negatively correlated with all Eubacteria and the Bacteroides-Prevotella group, while tumor necrosis factor alpha was not significantly correlated with these six gut bacteria in cirrhotic patients.CONCLUSIONS: Our study demonstrates that abundant gut bacteria were altered significantly in both cirrhotic and liver transplantation patients, while plasma endotoxin and interleukin-6 increased remarkably in cirrhotic patients, showing significant correlations with gut microbiota. Interestingly, our data show a tendency for these gut bacteria to restore to normal levels in liver transplantation recipients. | Zhong-Wen Wu, Zong-Xin Ling, Hai-Feng Lu, Jian Zuo, Ji-Fang Sheng, Shu-Sen Zheng and Lan-Juan Li State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Department of Infectious Diseases Key Lab of Combined Multiorgan Transplantation, Ministry of Public Health, Key Lab of Organ Transplantation and Department of Hepatobiliary and Pancreatic Surgery , First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，1： | 37 |
| 14 | Immunohistochemical study on p53,H-rasp21,c-erbB-2 protein and PCNA expression in HCC tissues of Hart and minority ethnic patients显示文摘AIM To find out the difference of humanprimary liver carcinogenesis between Han andminority ethnic patients in Xinjiang.METHODS Expression of p53,c-erbB-2,H-rasp21 protein and proliferating cell nuclearantigen(PCNA)in tumor tissues of 50 patients(Han 38,minority 12)with primary hepaticcarcinoma was detected byimmunohistochemistry(LSAB).RESULTS The positive frequency of p53,c-erbB-2,H-rasp21 and PCNA expression was46.0%(23/50),70.0%(35/50),68.0%(34/50)and 82.0%(41/50)in tumor tissues;4.0%(2/50),22.0%(11/50),64.0%(32/50)and 52.0%(26/ 50)in peritumors respectively and asignificant difference,except for H-rasp21,ofoncogene alteration was found(P<0.05)between tumor and non-tumorous tissues.Combined the three oncogenes alteration,26%(13/50)tumor tissues had positiveimmunoreactivity,but in peritumor and normallivers it was negative.The positive rate of p53,c-erbB-2 and H-rasp21 protein expression was39.5%(15/38),60.5%(23/38)and 39.5%(15/38)in tumors of Han patients;66.7%(8/12),100%(12/12)and 75.0%(9/12)in minoritiesrespectively,with statistical difference (P<0.05).CONCLUSION Overexpression of p53,c-erbB-2and H-rasp21 in human primary liver carcinoma isan important biomarker of genetic alteration.The different frequency of these oncogeneticchanges may reflect some environmental or/andethnic hereditary factors affecting the livercarcinogenesis.The special life style of Han,Uygur,Kazak and Mongolia nationalities inXinjiang may also be related to theetiopathogenesis of this disease. | Guo Yue Lin Zhao Lun Chen Cai Mo Lu Ying Li Xiao Jia Ping Rong Huang Department of Laboratory Medicine,Chinese PLA 474 Hospital,Urumqi 830011,Xinjiang Uygur Autonomic Region,China Department of Pathology,the 1st Teaching Hospital,Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomic Region,China | 2000 | World Journal of Gastroenterology2000，6，2： | 40 |
| 15 | Morphological and functional changes of mitochondria in apoptotic esophageal carcinoma cells induced by arsenic trioxide显示文摘AIM:To demonstrate that mitochondrial morphological andfunctional changes are an important intermediate link in thecourse of apoptosis in esophageal carcinoma calls inducedby As_2O_3.METHODS:The esophageal carcinoma call line SHEEC1,established in our laboratory,was cultured in 199 growthmedium,supplemented with 100mL·L^(-1)calf serum and3 mol·L^(-1)As_2O_3(the same below).After 2,4,6,12,24 hof drug adding,the SHEECl calls were collected for light-and electron-microscopic examination.The mitochondriawere labeled by Rhodamine fluorescence probe and thefluorescence intensity of the mitochondria was measured byflow cytorneter and cytofluorimetric analysis.Further,themitochondrial transmembrane potential(MTP,ΔΨm)change was also calculated.RESULTS:The mitochondrial morphological change afteradding As_2O_3 could be divided into three stages.In theearly-stage(2-6h)after adding As203,an adaptiveproliferation of mitochondria appeared;in the mid-stage(6-12 h)a degenerative change was observed;and in the late-stage(12-24 h)the mitochondria swelled with outermembrane broken down and then calls death with apoptoticchanges of nucleus.The functional change of themitochondria indicated by fluorescent intensity,whichreflected the MTP status of mitochondria,was in accordancewith morphological change of the mitochondria.Thefluorescent intensity increased at early-stage,decUned inmid-stage and decreased to the lowest in the late-stage.24h after As_2O_3 adding,the cell nucleus showed typicalapoptotic changes.CONCLUSION:Under the inducement of As_2O_3,the earlyapoptotic changes of SHEEC1 cells ware the apparentmorphological and functional changes of mitochondria,afterwards the nucleus changes followed.It is consideredthat changes of mitochondria are an important intermediatelink in the course of apoptosis of esophageal carcinoma cells induced by As_2O_3. | Zhong-Ying Shen Jian Shen Qiao-Shan Li Department of Pathology,Medical College of Shantou University,Shantou 515031,Guandong Province,China Cai-Yun Chen Central Lab.Medical College of Shantou University Jiong-Yu Chen Central Lab.of Tumor Hospital,Medical College of Shantou University Yi Zeng Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China | 2002 | World Journal of Gastroenterology2002，8，1： | 35 |
| 16 | Effects of the tyrosine protein kinase inhibitor genistein on the proliferation,activation of cultured rat hepatic stellate cells显示文摘AIM: Hepatic stellate cell (HSC) plays a pivotal role inliver fibrosis and is considered as the therapeutic targetfor the treatment of hepatic fibrosis. Tyrosine proteinkinase plays an important role in the proliferation,activation of HSC. The purpose of the study is toinvestigate the effects of the tyrosine protein kinaseinhibitor genistein onthe proliferation and activationof cultured rat HscMETHODS: Rat HSC were isolated from Wistar rats by insitu perfusion of collagenase and pronase and single-stepdensity Nycodenz gradient. Culture-activated HSC wereserum-starved and incubated with 10-3 to 10-5 mol/Lconcentration of genistein for 24, 48 or 72 h. In PDGF-induced HSC proliferation, HSC were stimulatel with L0μg@L-1PDGF-BB for 15 min, and then treaeed with genisteinfor the same time. Cell proliferation was measured byMTr assay and based on flow cytometric analysis of cellcycle. The a-smooth muscle actin (α-SMA) expression inHSC was studied with confocal laser microscopy and flowcytometry. c-fos c-jun and cyclin D1 expression in HSCwas also detected by flow cytometry.RESULTS: Genistein inhibited basal and PDGF-inducedproliferation of HSC at the concentration of 10-8 to 10-5mol/L, and treatment with 10-7 mol/L concentration ofgenistein for 48 h inhibited the HSC proliferationsignificantly (the inhibition rate was 70.3 %, P<0.05).Immunofluorescence detected by confocal lasermicroscopy and flow cytometry showed that treatmentwith 10-7 mol/L genistein for 48 h suppressed theexpression of α-SMA significantly in HSC (the specificfluorescence intensity were 60.2±21.5 vs 35.3±11.6and 12.8± 10.4 vs9.54±6.39, respectively, both P<0.05).The intensity of c-fos, c-jun and cydin D1 expression ofHSCs treated with 10-7 mol/L genistein for 48 h wasalso significantly decreased compared with the controls.CONCLUSION: Genistein influences proliferation of HSC,suppresses the expression of α-SMA in HSC and tinhibits the intensity of c-fos, c-jun and cyclin D1expression of HSCs. Genistein has therapeutic potentialagainst liver fibrosis. | Xiao-Jing Liu Ming-Hui Huang Laboratory of Department of Internal Medicine,West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China Li Yang Qiong Wang Yi-Ping Wang Department of Gastroenterology of West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China Yong-Qiu Mao Center for Cancer Biotherapy of West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China Hong-Bin Wu Laboratory of Department of Surgery,West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 38 |
| 17 | Assessment of candidate plant DNA barcodes using the Rutaceae family显示文摘DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention.Here,seven regions (psbA-trnH,matK,ycf5,rpoC1,rbcL,ITS2,and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae.To evaluate each barcode’s utility for species authentication,PCR amplification efficiency,genetic divergence,and barcoding gaps were assessed.We found that the ITS2 region exhibited the highest inter-specific divergence,and that this was significantly higher than the intra-specific variation in the 'DNA barcoding gap' assessment and Wilcoxon two-sample tests.The ITS2 locus had the highest identification efficiency among all tested regions.In a previous study,we found that ITS2 was able to discriminate a wide range of plant taxa,and here we confirmed that ITS2 was also able to discriminate a number of closely related species.Therefore,we propose that ITS2 is a promising candidate barcode for plant species identification. | LUO Kun1,2,CHEN ShiLin1,CHEN KeLi2,SONG JingYuan1,YAO Hui1,MA XinYe1,ZHU YingJie3,PANG XiaoHui1,YU Hua1,LI XiWen1,4 & LIU Zhen2 1Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China 2College of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430061,China 3School of Bioscience and Engineering,Southwest Jiaotong University,Chengdu 610031,China 4Department of Chemistry,Tsinghua University,Beijing 100084,China | 2010 | Science China(Life Sciences)2010，53，6： | 32 |
| 18 | Challenges in Research and Development of Traditional Chinese Medicines显示文摘This review is briefly to recall the history of research and development (R&D) of Chinese materia medica (CMM) and to discuss the challenges of Chinese traditional and herbal medicines (CTHM) facing the modern science and technology. The R&D of CTHM is thought to be an important pathway for new drug discovery. Since1949, about 140 approved new drugs have been developed, among which about 80 originated directly or indirectly from medicinal plants. CTHM has gained interest from the international medical, biomedical, and pharmaceutical institutions as a valuable source of potential medicines. For the modernization of CMM and innovative research of CTHM, there are following challenges to be faced: (1) to evaluate the efficacy, pharmacological properties, action mechanism, and active chemical constituents; (2) to develop new methodologies for the quality and safety of CTHM; (3) to apply new '-omics' techniques to accelerate drug discoveries developed from CTHM; and (4) to apply international practices including good agricultural practice, good manufacturing practice, good laboratory practice, and good clinical practice in the R&D of CTHM. | LIU Chang-xiao1*, XIAO Pei-gen2, PENG Yong2, SONG Nai-ning1 1. Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China | 2009 | Chinese Herbal Medicines2009，1，1： | 34 |
| 19 | Targeting Gene-Virotherapy of Cancer and its prosperity显示文摘为癌症的基因和病毒的治疗显示出一些治疗学的效果,但是有真实突破的缺乏。为了达到肿瘤的完全的消除的目标,在动物的异种皮移植当模特儿,我们开发了叫的新策略指向癌症的 Gene-Virotherapy,它试图联合基因治疗和 virotherapy 的优点。这新策略独自比基因或病毒的治疗生产了更强壮的反肿瘤效果。肿瘤特定的 replicative 侵入人体气管粘膜的病菌向量,指定了为 ZD55,被侵入人体气管粘膜的病菌的 55kDa E1B 区域的删除构造。结果病毒的构造不仅由明确地指向 p53 保留类似的功能到 ONYX-015 否定肿瘤,而且允许各种各样的治疗学的基因到的插入由于最新介绍的克隆的地点形成适当 ZD55 衍生物,与原来的 ONYX-015 病毒不可行的一项任务。我们证明反肿瘤一如此的衍生物完成, ZD55-IL-24,比 ZD55 virotherapy 或 Ad-IL-24 基因治疗的是乘更多的有势力的至少 100。不过,由 ZD55-IL-24 的使用的肿瘤质量的完全的消除是仅仅在一些然而并非所有老鼠观察了,显示那一治疗学的基因不对“痊愈”足够这些老鼠。有互补或合作的效果的基因是什么时候,独立克隆进 ZD55 向量并且在联合使用了(指定了为双基因治疗策略) ,好一些的结果被获得;并且如果二合适的基因被选择,在所有老鼠完成所有异种皮移植肿瘤群众的完全的消除是可能的。更全面的研究将多半为临床的试用基于这新策略导致一个协议。最后,为癌症治疗的两倍控制指向的病毒双的基因治疗的概念,和在癌症干细胞的最近的进步的含意也被讨论。 | Xin Yuan Liu~(1,2) ~1Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,320 Yue Yang Road,Shanghai 200031,China ~2Xinyuan Institute of Medicine and Biotechnology,School of Life Science,Zhejiang Sci-Tech University,Hangzhou 310018,China | 2006 | Cell Research2006，16，11： | 32 |
| 20 | 乳腺癌分期辨证规范(试行)显示文摘 | Working Committee of Mastopathy,All-China Society of Traditional Chinese Medicine | 2010 | 上海中医药杂志2010，44，1： | 33 |
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