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| 1 | Petrogenesis and dating of the Kangding complex,Sichuan Province显示文摘An analysis of major and trace element geochemistry and SHRIMP U-Pb zircon dating of mafic, intermediate, and felsic rocks from the Mianning metamorphic complex of Si- chuan Province was conducted. One of our conclusions is that a majority of the zircons crystal- lized between 721 and 773 Ma in a magmatic regime. The complexes contain early to late Pa- leo-proterozoic relict zircons from ancient continental crust. The oldest age, 2468 Ma, may rep- resent the basement of the Yangtze block. Overgrowth zircon rims and palingenetic zircons have been observed and dated, and are believed to have formed after crystallization, during Paleozoic and Mesozoic metamorphic-anatexis. We also conclude that depletion of Nb, Ta, HREE, in a spider-diagram normalized to primitive mantle and calc-alkaline association, implies that they formed in island-arc or under-plating settings. | CHEN Yuelong 1 , LUO Zhaohua 1 , ZHAO Junxiang, LI Zhihong, ZHANG Hongfei & SONG Biao 3 1. Faculty of Earth Sciences and Natural Resources, China University of Geosciences, Beijing 100083, China 2. Faculty of Earth Sciences, China University of Geosciences, Wuhan 430074, China 3. Beijing SHRIMP Center, Institute of Geology, Chinese Academy of Geological Sciences, Beijing 100037, China | 2005 | Science China Earth Sciences2005，48，5： | 40 |
| 2 | Invasive mechanism and management strategy of Bemisia tabaci(Gennadius) biotype B:Progress report of 973 Program on invasive alien species in China显示文摘Bemisia tabaci(Gennadius) biotype B,called a 'superbug',is one of the most harmful biotypes of this species complex worldwide.In this report,the invasive mechanism and management of B.tabaci biotype B,based on our 5-year studies,are presented.Six B.tabaci biotypes,B,Q,ZHJ1,ZHJ2,ZHJ3 and FJ1,have been identified in China.Biotype B dominates the other biotypes in many regions of the country.Genetic diversity in biotype B might be induced by host plant,geographical conditions,and/or insecticidal application.The activities of CarE(carboxylesterase) and GSTs(glutathione-S-transferase) in biotype B reared on cucumber and squash were greater than on other host plants,which might have increased its resistance to insecticides.The higher activities of detoxification enzymes in biotype B might be induced by the secondary metabolites in host plants.Higher adaptive ability of biotype B adults to adverse conditions might be linked to the expression of heat shock protein genes.The indigenous B.tabaci biotypes were displaced by the biotype B within 225 d.The asymmetric mating interactions and mutualism between biotype B and begomoviruses via its host plants speed up widespread invasion and displacement of other biotypes.B.tabaci biotype B displaced Trialeurodes vaporariorum(Westwood) after 4-7 generations under glasshouse conditions.Greater adaptive ability of the biotype B to adverse conditions and its rapid population increase might be the reasons of its successful displacement of T.vaporariorum.Greater ability of the biotype B to switch to different host plants may enrich its host plants,which might enable it to better compete with T.vaporariorum.Native predatory natural enemies possess greater ability to suppress B.tabaci under field conditions.The kairomones in the 3rd and 4th instars of biotype B may provide an important stimulus in host searching and location by its parasitoids.The present results provide useful information in explaining the mechanisms of genetic diversity,evolution and molecular eco-adaptation of biotype B.Furthermore,it provides a base for sustainable management of B.tabaci using biological and ecological measures. | WAN FangHao1,ZHANG GuiFen1,LIU ShuSheng2,LUO Chen3,CHU Dong4,ZHANG YouJun4,ZANG LianSheng2,JIU Min2,Lü ZhiChuang1,CUI XuHong1,ZHANG LiPing4,ZHANG Fan3,ZHANG QingWen5,LIU WanXue1,LIANG Pei5,LEI ZhongRen1 & ZHANG YongJun1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Acadeny of Agriculture Sciences,Beijing 100094,China 2 Institute of Applied Entomology,Zhejiang University,Hangzhou 310029,China 3 Institute of Plant and Environment Protection,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100089,China 4 Institute of Vegetables and Flowers,Chinese Acadeny of Agricullare Sciences,Beijing 100081,China 5 Department of Entomology,China Agriculture University,Beijing 100094,China | 2009 | Science China(Life Sciences)2009，52，1： | 30 |
| 3 | Efects of a bioartificial liver support system on a cetaminophen-induced acute liver failure canines显示文摘Seeinvitedcommentaryonpage286Subjectheadingsliversupportsystem;acuteliverfailure;canines;porcinehepatocytes;bioartificiallive... | XUE Yi Long 1, ZHAO Shi Feng 2, ZHANG Zuo Yun 1, WANG Yue Feng 1, LI Xin Jian 1, HUANG Xiao Qiang 3, LUO Yun 1, HUANG Ying Cai 4 and LIU Cheng Gui 1 | 1999 | World Journal of Gastroenterology1999，5，4： | 19 |
| 4 | Preliminary study on the origin identification of natural gas by the parameters of light hydrocarbon显示文摘The light hydrocarbon composition of 209 natural gas samples and individual light hydrocarbon carbon isotopes of 53 natural gas samples from typical humic-sourced gas and sapropelic-sourced gas in the four basins of China have been determined and analyzed.Some identification parameters for humic-sourced gas and sapropelic-sourced gas are proposed or corrected.The differences of compound-specificδ13C value of individual light hydrocarbon between humic-sourced gas and sapropelic-sourced gas have been founded.The humic-sourced gas has the distribution ofδ13C benzene > -24‰,δ13C toluene >-23‰,δ13C cyclohexane >-24‰andδ13C methyl cyclohexane >-24‰,while the sapropelicsourced gas has the distribution ofδ13C benzene <-24‰,δ13C toluene <-24‰,δ13C cyclohexane <-24‰and δ13C methyl cyclohexane <-24‰.Among the components of C7 light hydrocarbon compound,such as normal heptane(nC7),methyl cyclohexane(MCH)and dimethyl cyclopentane(ΣDMCP),etc,relative contents of nC7 and MCH are influenced mainly by the source organic matter type of natural gas.Therefore,it is suggested that the gas with relative content of nC7 of more than 30%and relative content of MCH of less than 70%is sapropelic-sourced gas,while gas with relative content of nC7 of less than 35%and relative content of MCH of more than 50%is humic-sourced gas.Among components of C5-7 aliphatics, the gas with relative content of C5-7 normal alkane of more than 30%is sapropelic-sourced gas,while the gas with relative content of C5-7 normal alkane of less than 30%is humic-sourced gas.These paremeters have been suggested to identify humic-sourced gas and sapropelic-sourced gas. | HU GuoYi 1,2,3 ,LI Jian 1,2 ,LI Jin 1,2 ,LI ZhiSheng 1,2 ,LUO Xia 1,2 ,SUN QingWu 1,2 &MA ChengHua 1,2 1 Research Institute of Petroleum Exploration and Development(Langfang Branch),Petrochina,Langfang 065007,China 2 The Key Laboratory of Gas Formation and Development,Petrochina,Langfang 065007,China 3 College of Geo-Resources and Information,China University of Petroleum,Beijing 102200,China | 2008 | Science China Earth Sciences2008，51，S1： | 19 |
| 5 | Influence of stacking fault energy on formation of long period stacking ordered structures in Mg-Zn-Y-Zr alloys | Fu-sheng PAN~(1,2),Su-qin LUO~1,Ai-tao TANG~(1,2),Jian PENG~(1,2),Yun LU~3 1.College of Materials Science and Engineering,Chongqing University,Chongqing 400044,China 2.National Engineering Research Center for Magnesium Alloys,Chongqing University,Chongqing 400044,China 3.Faculty of Engineering,Chiba University,Chiba 263-8522,Japan | 2011 | Progress in Natural Science:Materials International2011，21，6： | 17 |
| 6 | Simulation of freezing and melting of soil on the northeast Tibetan Plateau显示文摘Using observational data spanning the period from February to December 2009 and recorded at the Suli station in Qinghai Province,the land-surface model CLM3.0 was employed to simulate the freezing and melting of soil.The results indicate that the simulated soil temperature is higher than the observed soil temperature and the ultimate thawing date is earlier than the observed date during the melting period.During the freezing period,the simulated soil temperature is lower than the observed soil temperature and the ultimate freezing of the deep soil is earlier than that observed.Overall,the simulation of freezing is better than that of melting,and the simulation of a shallow layer is better than that of a deeper layer.In the original CLM3.0,it is assumed that frozen soil begins to melt when the soil temperature exceeds 0C,which is inconsistent with observations.The critical freeze-thaw temperature was calculated according to thermodynamics equations and the freeze-thaw condition was modified.In this work,the melting rate for frozen soil was reduced using the modified scheme,and the simulated soil temperature was lowered. Meanwhile,the refreezing of soil during the melting season was well simulated and more closely matched observations.Additionally,it was found that the rates of melting and freezing differ,with the former being slower than the latter,but refreezing during the melting season is rather quick. | XIA Kun 1,2,3,LUO Yong 2,4,5 & LI WeiPing 2 1 Institute of Atmospheric Physics,Chinese Academy of Sciences,Beijing 100029,China 2National Climate Center,China Meteorological Administration,Beijing 100081,China 3Graduate University of Chinese Academy of Sciences,Beijing 100049,China 4 Center for Earth System Science,Tsinghua University,Beijing 100084,China 5Key Laboratory of Earth System Numerical Simulation,Ministry of Education,Beijing 100084,China | 2011 | Chinese Science Bulletin2011，56，20： | 14 |
| 7 | Diet and gastric cancer:a case-control study in Fujian Province,China显示文摘NTRODUCTIONChangleandFuqingcitiesarelocatedinthesoutheasternpartofFujianProvince,Chinawithahighincidenceofgastriccancer.Howev... | YE Wei Min 1, YI Ying Nan 1, LUO Ren Xia 2, ZHOU Tian Shu 3, LIN Ru Tao 1 and CHEN Gui Dong 3 | 1998 | World Journal of Gastroenterology1998，4，6： | 12 |
| 8 | A LOOP DRAWBEAD RESTRAINING FORCE MODEL IN SHEET METAL DEEP DRAWING PROCESS显示文摘In sheet metal forming, drawbeads are often used to control uneven material flow which may cause defects such as wrinkles, fracture, surface distortion and springback. Appropriate setting and adjusting the drawbead force is one of the most important pa- rameters in sheet metal forming process control. This paper derives the model of loop drawbead restraining force (DBRF) using plastic forming theory, and gives the min imum blankholding force. Experiments are carried out to verify the proposed DBRF model. The good agreement between the calculated values and experiments data justi- fies the proposed loop drawbead restraining force model. | Y.J. Luo, S.P. Li, J.F. Zheng, D.N. He, Y.Q. Zhang, J.L. Cheng and J. Y. Jiang 1)National Mold & Die CAD Engineering Research Center, Shanghai 200030, China 2)Shanghai Volkswagen Automotive Company Ltd.(SVM), Shanghai 201805, China | 2001 | Acta Metallurgica Sinica(English Letters)2001，14，5： | 11 |
| 9 | High frequencies of HGV and TTV infections in blood donors in Hangzhou显示文摘ALM To determine the frequencies of HGV and TTVinfections in blood donors in Hangzhou.METHODS RT-nested PCR for HGV RNA detection and semi-nested PCR for TTV DNA detection in the sera from 203 blood donors, and nucleotide sequence analysis were performed.``RESULTS Thirty-two ( 15.8%) and 30 (14.8%) of the 203serum samples were positive for HGV RNA and TTV DNA,respectively. And 5 (2.5%) of the 203 serum samples were detectable for both HGV RNA and TTV DNA.Homology of the nucleotide sequences of HGV RT-nested PCR products and TTV semi-nested PCR products from 3serum samples compared with the reported HGV and TTV sequences was 89.36%. 87.94%, 88.65% and 63.51%.65.77% and 67.12%. respectively.``CONCLUSION The infection rates of HGV and/or TTV inblood donors are relatively high. and to establish HGV and TTV examinations to screen blood donors is needed for transfusion security. The genomic heterogeneity of TTV or HGV is present in the isolates from different areas. | Jie Yan~1 Li-Li Chen~2 Yi-Hui Luo~1 Ya-Fei Mao~1 Meng He~1 1 Department of Pathogenic Biology,Medical School of Zhejiang University,Hangzhou 310006,Zhejiang Province,China2 Department of Stomatology,The Second Affiliated Hospital,Medical School of Zhejiang University,Hangzhou 310009,Zhejiang Province,China | 2001 | World Journal of Gastroenterology2001，7，5： | 11 |
| 10 | Grey GM(1,1) Model with Function-Transfer Method for Wear Trend Prediction and its Application显示文摘Trend forecasting is an important aspect in fault diagnosis and work state supervision. The principle, where Grey theory is applied in fault forecasting, is that the forecast system is considered as a Grey system; the existing known information is used to infer the unknown information’s character, state and development trend in a fault pattern, and to make possible forecasting and decisions for future development. It involves the whitenization of a Grey process. But the traditional equal time interval Grey GM (1,1) model requires equal interval data and needs to bring about accumulating addition generation and reversion calculations. Its calculation is very complex. However, the non equal interval Grey GM (1,1) model decreases the condition of the primitive data when establishing a model, but its requirement is still higher and the data were pre processed. The abrasion primitive data of plant could not always satisfy these modeling requirements. Therefore, it establishes a division method suited for general data modeling and estimating parameters of GM (1,1), the standard error coefficient that was applied to judge accuracy height of the model was put forward; further, the function transform to forecast plant abrasion trend and assess GM (1,1) parameter was established. These two models need not pre process the primitive data. It is not only suited for equal interval data modeling, but also for non equal interval data modeling. Its calculation is simple and convenient to use. The oil spectrum analysis acted as an example. The two GM (1,1) models put forward in this paper and the new information model and its comprehensive usage were investigated. The example shows that the two models are simple and practical, and worth expanding and applying in plant fault diagnosis. | LUO You xin 1 , PENG Zhu 2 , ZHANG Long ting 1 , GUO Hui xin 1 , CAI An hui 1 1Department of Mechanical Engineering, Changde Teachers University, Changde 415003, P.R. China 2 Engineering Technology Board, Changsha Cigare | 2001 | International Journal of Plant Engineering and Management2001，6，4： | 11 |
| 11 | Sr-Nd-Pb isotopes of the Early Paleozoic maficultramafic dykes and basalts from South Qinling belt and their implications for mantle composition显示文摘Late Early Paleozoic mafic-ultramafic dykes and volcanic rocks from the South Qinling belt are char- acterized by εNd( t ) = +3.28― +5.02, (87Sr/86Sr)i= 0.70341― 0.70555, (206Pb/204Pb)i = 17.256― 18.993, (207Pb/204Pb)i= 15.505―15.642, (208Pb/204Pb)i=37.125―38.968, ?8/4=21.18―774.43, ?7/4=8.11―18.82. These charac- teristics suggest that they derived from a Middle Neoproterozoic mantle with isotopic compositions of mixed HIMU, EMII and minor EMI components. We interpret that these rocks were melting products of depleted mantle modified by subducted ancient oceanic crust and continental margin sediments along the northern margin of Yangtze block during Early Neoproterozoic. | ZHANG ChengLi1,2, GAO Shan1, 2, YUAN HongLin1, ZHANG GuoWei1, YAN YunXiang1, LUO JingLan1 & LUO JinHai1 1 State Key Laboratory of Continental Dynamics, Department of Geology, Northwest University, Xi’an 710069, China 2 State Key Laboratory of Geological Progresses and Mineral Resources, China University of Geosciences, Wuhan 430074, China | 2007 | Science China Earth Sciences2007，50，9： | 10 |
| 12 | A new grid-associated algorithm in the distributed hydrological model simulations显示文摘This paper presents a new grid-associated algorithm to improve the performance of a D8 algorithm based distributed hydrological model computation.The algorithm is based on the well known single-flow D8 algorithm of grid flow.This algorithm allocates calculation priorities according to the distance between the units and the outlet,then carries out the ergodic computations of the hydrological units according to the priority division.For the parallelized algorithm,a standard thread-level shared memory system for parallel programming(OpenMP-Open specifications for Multi Processing) was introduced,and the parallel coding was implemented in C lan-guage.A case study showed that the absolute speed-up ratio of the grid-associated algorithm is 1.64 over the original D8 algorithm,and the linear speed-up ratio of the parallel associated algorithm is 2.42 under 4 cores.The parallel grid-associated algorithm can be applied to a variety of research fields that use the grid method. | XU Rui1,2,HUANG XiaoXue1,LUO Lin1 & LI ShaoCai3 1 State Key Laboratory of Hydraulics and Mountain River Engineering College of Architecture and Environment,Sichuan University,Chengdu 610065,China 2 College of Electronic Engineering,Guilin University of Electronic Technology,Guilin 541004,China 3 College of Life Sciences,Sichuan University,Chengdu 610065,China | 2010 | Science China(Technological Sciences)2010，53，1： | 8 |
| 13 | A new orthogonality relationship for orthotropic thin plate theory and its variational principle显示文摘The thought how dual vectors are constructed in a new orthogonality relationship for theory of elasticity is generalized into orthotropic thin plate bending problems by using the analogy theory between plane elasticity problems and plate bending problems. Dual differential equations are directly obtained by using a mixed variables method. A dual differential matrix to be derived possesses a peculiarity of which principal diagonal sub-matrixes are zero matrixes. Two independently and symmetrically orthogonality sub-relationships are discovered. By using the integral form for elastic bending theory of orthotropic thin plate the orthogonality relationship is demonstrated. By selecting felicitous dual vectors a new orthogonality relationship for theory of elasticity can be generalized into elastic bending theory of orthotropic thin plate. By using the integral form a variational principle which is relative to differential form and a whole function expression are proposed. | LUO Jianhui 1 , LONG Yuqiu 2 & LIU Guangdong 1 1. College of Civil Engineering, Hunan University, Changsha 410082, China 2. Department of Civil Engineering, Tsinghua University, Beijing 100084, China | 2005 | Science China(Physics,Mechanics & Astronomy)2005，48，3： | 8 |
| 14 | The role of MC1R gene in buffalo coat color显示文摘Melanocortin-1 receptor (MC1R) plays a major role in pigmentation in many species.To investigate if the MC1R gene is associated with coat color in water buffalo,the coding region of MC1R gene of 216 buffalo samples was sequenced,which included 49 black river buffalo (Murrah and Nili-Ravi),136 swamp buffalo (Dehong,Diandongnan,Dechang,Guizhou,and Xilin) with white and gray body,and 31 hybrid offspring of river buffalo Nili-Ravi (or Murrah) and swamp buffalo.Among the three variation sites found,SNP684 was synonymous,while SNP310 and SNP384 were nonsynonymous,leading to p.S104G and p.I128M changes,respectively.Only Individuals carrying homozygote EBR/EBR were black.The genotype and phenotype analysis of the hybrid offspring of black river buffalo and gray swamp buffalo further revealed that the river buffalo type allele EBR or the allele carrying the amino acid p.104S was important for the full function of MC1R.The in silico functional analysis showed that the amino acid substitutions p.G104S and p.M128I had significant impact on the function of MC1R.Above results indicate that the allele EBR or the allele carrying the amino acid p.104S was associated with the black coat color in buffalo. | MIAO YongWang1,2,WU GuiSheng3,WANG Lei2,LI DaLin4,TANG ShouKun5,LIANG JianPing2,MAO HuaMing2,LUO HuaiRong3 & ZHANG YaPing1,6 1 Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China 2 Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China 3 Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China 4 Yunnan Institute of Buffalo Science and Technology,Kunming 650021,China 5 Animal Husbandry and Veterinary Station of Luxi city,Luxi 678400,China 6 State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,China | 2010 | Science China(Life Sciences)2010，53，2： | 8 |
| 15 | Repair effect of diabetic ulcers with recombinant human epidermal growth factor loaded by sustained-release microspheres显示文摘In this study the w/o/w extraction–evaporation technique was adopted to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres loading recombinant human epidermal growth factor (rhEGF). The micro-spheres were characterized for morphology by transmission electron microscopy (TEM) and particle size distribution. The release performances, the proliferation effects and therapeutic effects of rhEGF-loaded PLGA microspheres were all studied. The results showed that these spherical micro-spheres had a narrow size distribution and a high drug encapsulation efficiency (85.6%). RhEGF-loaded microspheres enhanced the growth rate of fibroblasts and wound healing more efficiently than pure rhEGF. The number of the proliferating cell nuclear antigen (PCNA) in the epidermis layer with the mi-crosphere treatment was significantly larger than those of the control groups. Overall locally sustained delivery of rhEGF from biodegradable PLGA microspheres may serve as a novel therapeutic strategy for diabetic ulcer repair. | DONG XiaoQing1, XU Jun2, WANG WeiCai1, Luo Hao1, LIANG XiaoFei1, Zhang Lei1, Wang HanJie1, Wang PengHua2 & CHANG Jin1 1 Institute of Nanobiotechnology, School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China 2 Department of Diabetic Foot, Tianjin Medical University Metabolic Disease Hospital, Tianjin 300070, China | 2008 | Science China(Life Sciences)2008，51，11： | 7 |
| 16 | Transmission electron microscopic observationof Au^(3+) biosorption by Saccharomycescerevisiae waste biomass显示文摘本文报告啤酒酵母废菌体对 Au3 +的吸附还原作用。该菌体在金离子起始浓度 2 0 0μg/ ml,菌体浓度2 mg/ ml,p H3 .0和 3 0℃的条件下 ,吸附 60 min,吸附量达 55.9mg/ g干菌体。 TEM观察结果表明 ,溶液中的Au3 +离子能被啤酒酵母废菌体吸附 ,并还原生成 Au0 颗粒。所生成的 Au0 颗粒能形成不同形状和大小的金晶体。吸附在 α- Fe2 O3 和 Si O2 上的 Au3 +离子也能被啤酒酵母废菌体还原形成纳米大小的金颗粒。 | LIU Yue-ying 1 FU Jin-kun 2 LUO Xue-feng 1 NI Zi-mian 3 CHEN Ping 3 YU Xin-sheng 2 ( 1 Department of Biology, 2 Department of Chemistry, 3 Centre of Analysis & Testing, Xiamen University, Xiamen 361005, P. R. China) | 2000 | 电子显微学报2000，19，5： | 7 |
| 17 | A preliminary study on dead geostationary satellite removal显示文摘The collision between satellites IRIDIUM 33 and COSMOS 2251 indicated that the clash of two on-orbit satellites was becoming an inevitable reality. Our calculation with the two-line orbit element by NORAD showed that some two geostationary satellites had approached very close in July 2009. Therefore, more attention should be given to avoid such collisions. This paper analyzes the orbital long-term variation of a dead satellite drifting in the geostationary orbit. Also, the negative effects posed by dead satellites upon the on-orbit operational geostationary satellites are studied. Then the paper proposes a novel idea to launch a satellite sweeper whose purpose is to collect the on-orbit dead satellites and help them de-orbit to a 'graveyard'. The satellite sweeper consists of a parent satellite and a child satellite. The child satellite collects a dead satellite and transfers it to a higher orbit. The parent satellite stationed in the geostationary orbit is in charge of refueling the child satellite. The strategy of maneuver and rendezvous is presented and a series of formulas are derived. The analysis results show that our method to clean the geostationary orbital zone is practical and fuel-saving. With the help of just a few satellite sweepers, we can gain a clean environment of geostationary orbit environment again. | LI YuHeng1, YANG KaiZhong2, SHAN ChengSheng2, LUO Dan2, GUAN Hui2, ZHENG Jun2, CHENG Hong2 & MAN Li2 1 Xidian University, Shaanxi 710071, China 2 Xi’an Satellite Control Center, Shaanxi 710043, China | 2010 | Science China(Technological Sciences)2010，53，12： | 7 |
| 18 | Engineering infectious foot-and-mouth disease virus in vivo from a full-length genomic cDNA clone of the A/AKT/58 strain显示文摘Two full-length genomic cDNA clones, pTA/FMDV and pCA/FMDV, were constructed that contained three point-mutants [A174G and A308G (not present in pTA/FMDV); T1029G] in the genome compared with the wild type A/AKT/58 strain of foot-and-mouth disease virus. These two viruses were rescued by co-transfection of pCA/FMDV with pCT7RNAP, which can express T7 RNA polymerase in BHK-21 cell-lines, or by transfection of the in vitro transcribed RNA. Their biological properties were analyzed for their antigenicity, virulence in suckling-mice (LD50) and growth kinetics in BHK-21 cells. The in vivo rescued viruses showed high pathogenicity for 3-day-old unweaned mice (LD50=10?7.5). However, the in vitro transcribed RNA derived from pTA/FMDV had lower pathogenicity for suckling-mice (LD50=10?6), and the in vivo transcribed RNA recovered from pCA/FMDV co-transfected with pCT7RNAP showed no significant differences from the wild type virus. These data showed that recovery of the infectious foot-and-mouth disease virus directly from the use of in vivo techniques was better than from in vitro methods. Furthermore, the reverse genetic procedure technique was simplified to a faster one-step procedure based on co-transfection with pCT7RNAP. These results suggest that in vivo RNA tran- scripts may be more valuable for engineering recombinant foot-and-mouth disease virus than in vitro RNA transcripts, and may contribute to further understanding of the biological properties, such as replication, maturation and quasispecies, of the foot-and-mouth disease virus. | BAI XingWen1, LI PingHua1, CAO YiMei1, LI Dong1, LU ZengJun1, GUO JianHong1, SUN DeHui1, ZHENG HaiXue2, SUN Pu1, LIU XiangTao1, LUO JianXun1 & LIU ZaiXin1 1 Key Laboratory of Animal Virology of Ministry of Agriculture, National Foot-and-Mouth Disease Reference Laboratory of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China 2 Veterinary Research Institute, Guangdong Agricultural Academy of Sciences, Guangzhou 510640, China | 2009 | Science China(Life Sciences)2009，52，2： | 6 |
| 19 | Expression of proliferating cell nuclear antigen in polyps from large intestine显示文摘Coloncancerhasahighincidenceintheworld,especialyinWesterncountries,andtheincidenceisalsoincreasinginChinainrecentyears.Toredu... | LUO Yu Qin 1, MA Lian Sheng 2, ZHAO Yi Ling 3, WU Kai Chun 4, PAN Bo Rong 5 and ZHANG Xue Yong 4 | 1999 | World Journal of Gastroenterology1999，5，2： | 6 |
| 20 | Wettability Modification for Biosurface of Titanium Alloy by Means of Sequential Carburization显示文摘Microporous titanium carbide coating was successfully synthesized on medical grade titanium alloy by using sequentialcarburization.Changes in the surface morphology of titanium alloy occasioned by sequential carburization were characterizedand the wettability characteristics were quantified.Furthermore,the dispersion forces were calculated and discussed.The resultsindicate that sequential carburization is an effective way to modify the wettability of titanium alloy.After the carburization thesurface dispersion force of titanium alloy increased from 76.5×10-3J·m-2 to 105.5×10-3 J·m-2,with an enhancement of37.9 %.Meanwhile the contact angle of titanium alloy decreased from 83° to 71.5°,indicating a significant improvement ofwettability,which is much closer to the optimal water contact angle for cell adhesion of 70°. | Yong Luo~(1,2) Shi-rong Ge~1 Zhong-min Jin~21.Institute of Tribology and Reliability Engineering,School of Material Science and Engineering,China University of Mining and Technology,Xuzhou 221008,P.R China2.Institute of Medical and Biological Engineering,School of Mechanical Engineering,University of Leeds,Leeds LS2 9JT,UK | 2009 | Journal of Bionic Engineering2009，6，3： | 6 |
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