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| 1 | Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis显示文摘AIM:To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental diversion colitis.METHODS:Wistar specific pathogen-free rats were submitted to a Hartmann's end colostomy and treated with enemas containing glutamine,butyrate,or saline.Enemas were administered twice a week in the excluded segment of the colon from 4 to 12 wk after the surgical procedure.Follow-up colonoscopy was performed every 4 wk for 12 wk.The effect of treatment was evaluated using video-endoscopic and histologic scores and measuring interleukin-1β,tumor necrosis factor-alpha,and transforming growth factor beta production in organ cultures by enzyme linked immunosorbent assay.RESULTS:Colonoscopies of the diverted segment showed mucosa with hyperemia,increased number of vessels,bleeding and mucus discharge.Treatment with either glutamine or butyrate induced significant reductions in both colonoscopic(P < 0.02) and histological scores(P < 0.01) and restored the densities of collagen fibers in tissue(P = 0.015;P = 0.001),the number of goblet cells(P = 0.021;P = 0.029),and the rate of apoptosis within the epithelium(P = 0.043;P = 0.011) to normal values.The high levels of cytokines in colon explants from rats with diversion colitis significantly decreased to normal values after treatment with butyrate or glutamine.CONCLUSION:The improvement of experimental diversion colitis following glutamine or butyrate enemas highlights the importance of specific luminal nutrients in the homeostasis of the colonic mucosa and supports their utilization for the treatment of human diversion colitis. | Rodrigo Goulart Pacheco Christiano Costa Esposito Lucas CM Müller Morgana TL Castelo-Branco Leonardo Pereira Quintella Vera Lucia A Chagas Heitor Siffert P de Souza Alberto Schanaider | 2012 | World Journal of Gastroenterology2012，18，32： | 9 |
| 2 | Therapeutic and prophylactic thalidomide in TNBS-induced colitis: Synergistic effects on TNF-α, IL-12 and VEGF production显示文摘AIM: To evaluated the therapeutic and prophylactic effect of thalidomide on 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Thalidomide has been reported to downregulate the expression of tumor necrosis factor a (TNF-α), IL-12, and vascular endothelial growth factor (VEGF), hallmarks of intestinal inflammation in Crohn's disease (CD). METHODS: Male Wistar rats were divided in five groups of ten animals each. Four groups received a rectal infusion of TNBS in ethanol. The first group was sacrificed 7 d after colitis induction. The second and third groups received either thalidomide or placebo by gavage and were sacrificed at 14 d. The fourth group received thalidomide 6 h before TNBS administration, and was sacrificed 7 d after induction. The fifth group acted as the control group and colitis was not induced. Histological inflammatory scores of the colon were performed and lamina propria CD4+ T cells, macrophages, and VEGF+ cells were detected by immunohistochemistry. TNF-a and IL-12 were quantified in the supernatant of organ cultures by ELISA. RESULTS: Significant reduction in the inflammatory score and in the percentage of VEGF+ cells was observed in the group treated with thalidomide compared with animals not treated with thalidomide. Both TNF-αand IL-12 levels were significantly reduced among TNBS induced colitis animals treated with thalidomide compared with animals that did not receive thalidomide. TNF-αlevels were also significantly reduced among the animals receiving thalidomide prophylaxis compared with untreated animals with TNBS-induced colitis. Intestinal levels of TNF-αand IL-12 were significantly correlated with the inflammatory score and the number of VEGF+ cells. CONCLUSION: Thalidomide significantly attenuates TNBS-induced colitis by inhibiting the intestinal production of TNF-α, IL-12, and VEGF. This effect may support the use of thalidomide as an alternate approach in selected patients with CD. | Ana Teresa Carvalho Heitor Souza Antonio Jose Carneiro Morgana Castelo-Branco Kalil Madi Alberto Schanaider Flavia Silva Fernando Antonio Pereira Júnior Márcia G Pereira Cláudio Tortori Ilana Dines Jane Carvalho Eduardo Rocha Celeste Elia | 2007 | World Journal of Gastroenterology2007，13，15： | 6 |
| 3 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 4 | Design of dedicated instrumentation for temperature distribution measurements in solid oxide fuel cells显示文摘 | Adzic M Heitor M V Santos D | 1997 | Journal of Applied Electrochemistry1997，27，12： | 1 |
| 5 | Swirl flow structure and flame characteristics in a model lean premixed combustor显示文摘 | Anacleto P M Fernandes E C Heitor M V | | 0，，08： | 1 |
| 6 | Corporate debt maturity and the real effects of the 2007 credit cri- sis显示文摘 | Heitor A Campello M Laranjeira B | 2012 | Critical Finance Review2012，1，1： | 1 |
| 7 | Building space–use analysis system — A multi location/multi sensor platform显示文摘 | Martijn Kuipers Ana Tomé Tiago Pinheiro Mário Nunes Teresa Heitor | 2014 | Automation in Construction2014，，： | 1 |
| 8 | Responses of chlorophyll fluorescence parameters of the facultative halophyte and C3 - CAM intermediate species Mesembryanthemum crystallinum to salinity and high irradiance stress 显示文摘 | Femando B Heitor M D Ulrich L | 2007 | Journal of Plant Physiology2007，164，7： | 1 |
| 9 | Design of dedicated instrumentation for temperature distribution measurements in solid oxide fuel cell 显示文摘 | ADZIC M HEITOR M V SANTOS D | 1997 | Journal of Applied Electrochemistry1997，27，12： | 1 |
| 10 | Expectations for the university in the Knowledge-based Economy 显示文摘 | Manuel V Heitor Pedro M Oliveira | 1998 | Technological Forecasting and Social Change1998，58，： | 1 |
| 11 | Financial Constraints,Asset Tangibility,and Corporate Investment显示文摘 | HEITOR M | 2007 | European Accounting Review2007，20，12： | 1 |
| 12 | Knowledge for InclusiveDevelopment显示文摘 | Conceicao P Gibson D V Heitor M V Sirilli G | 2009 | Technological Forecast-ing and Social Change2009，，1： | 1 |
| 13 | Swirl flow structure and flame characteristics in a model lean premixed combustor显示文摘 | Anacleto P M Fernandes E C Heitor M V | 2003 | Combustion Science and Technology2003，175，8： | 1 |
| 14 | Expectations for the university in the knowledge - based economy 显示文摘 | CONCEICAO P HEITOR M V OLIVERIRA P M | 1998 | Technological Forecasting and Social Change1998，，58： | 1 |
| 15 | Expectations for the university in the Knowledge-based Economy显示文摘 | Pedro Coneeicao Manuel V Heitor Pedro M Oliveira | 1998 | Technological Forecasting and Social Change1998，58，： | 1 |
| 16 | Velocity and scalar measurements in model and real gas turbine combustors 显示文摘 | HEITOR M V | 1989 | Instrumentation for Combustion and Flow in engines1989，154，： | 1 |
| 17 | Financial Constraints,Asset Tangibility,and Corporate Investment显示文摘 | HEITOR M | 2007 | European Accounting Review2007，20，3： | 1 |
| 18 | Responses of chlorophyll fluorescence parameters of the facuhative halophyte and C3-CAM intermediate species Mesembryaathemum crystallinum to salinity and high irradiance stress 显示文摘 | FERNANDO B HEITOR M D ULRICH L | 2007 | Journal of Plant Physiology2007，164，7： | 1 |
| 19 | Swirl flow structure and flame characteristics in a model lean premixed combustor显示文摘 | ANACLETO P M FERNANDES E C HEITOR M V | 2003 | Combustion Science and Technology2003，175，8： | 1 |
| 20 | Gravitational effects on the structure and propagation of premixedflame显示文摘 | HAMINS A HEITOR M LIBBY P A | 1988 | Acta Astronautica1988，17，5： | 1 |
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