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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Isolation and characterization of virus related to the SARS coronavirus from animals in southern china显示文摘 | Guan Y Zheng BJ He YQ | 2003 | Science2003，302，2： | 1 |
| 3 | Medication adherence in patients with rheumatoid arthritis:a critical ap- praisal of the existing literature显示文摘 | van den Bemt BJ Zwikker HE van den Ende CH | 2012 | Expet Rev Clin Immunol2012，8，4： | 1 |
| 4 | Transient isolated lesion of the splenium associated with clinically mild influenza encephalitis显示文摘 | Ganapathy S Elizabeth HE Wolfson BJ | 2008 | Pediatr Radiol2008，38，11： | 1 |
| 5 | Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China显示文摘 | Guan Y zhang BJ He YQ | 2003 | Science2003，302，： | 1 |
| 6 | Oxidation of CaMK Ⅱdetermines the cardiotoxic effects of aldosterone显示文摘 | He BJ Joiner ML Singh MV | | 0，，12： | 1 |
| 7 | Soybean isoflavone alleviates β-amyloid 1-42 induced inflammatory response to improve learning and memory ability by down regulation of Toll-like receptor 4 expression and nuclear factor-κB activity in rats显示文摘 | Ding BJ Ma WW He LL | | 0，，05： | 1 |
| 8 | Clinical feature of unintended thin corneal flap in LASIK:1-year follow-up显示文摘 | Yeo HE Song BJ | 2002 | Korean J Ophthalmol2002，16，： | 1 |
| 9 | Spatial variation in cartilage T2of the knee显示文摘 | Smith HE Modaer TJ Dardzimld BJ | 2001 | J Magn Reson Imaging2001，14，1： | 1 |
| 10 | Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China 显示文摘 | Guan Y Zheng BJ He YQ | 2003 | Science2003，302，： | 1 |
| 11 | Spatial variation in cartilage T2 of the knee显示文摘 | Smith HE Mosher TJ Dardzinski BJ | 2001 | J Magn Reson Imaging2001，14，1： | 1 |
| 12 | Spatial y variation in cartilage T2 of the knee显示文摘 | Smith HE Mosher TJ Dardzinski BJ | | 0，，01： | 1 |
| 13 | Oxidation of CaMKII determines the cardiotoxic effects of aldosterone显示文摘 | He BJ Joiner ML Singh MV | | 0，，12： | 1 |
| 14 | Six months results of argon laser gonioplasty显示文摘 | Crymes BM,Shingleton BJ | 1996 | Arch J Ophthalmol1996，118，1： | 1 |
| 15 | Stenotrophomonas maltophilia with multiple antimierobial resistance in hospital显示文摘 | Wang BQ Hu BJ He LX | 1999 | Chin J Nosocom1999，9，： | 1 |
| 16 | Serum inhibitor in systemic lupus erythematosus associated with aplastic anemia显示文摘 | Brooks BJ Jr Broxmeyer HE Bryan CF | 1984 | Arch Intern Med1984，144，7： | 1 |
| 17 | A picoliter-volume mixer for microfluidic analyti?cal systems显示文摘 | HE B BURK BJ | 2001 | Analytical Chemistry2001，73，9： | 1 |
| 18 | The efficacy and safety of ezetimibe and low-dose simvastatin as a primary treatment for dyslipidemia in renal transplant recipients 显示文摘 | Yoon HE Song JC Hyoung BJ | 2009 | Korean J Intern Med2009，24，3： | 1 |
| 19 | Activated Jak2 with the V617F point mutation promotes G1/S phase transition显示文摘 | Walz C Crowley BJ Hudon HE | 2006 | J Biol Chem2006，281，18： | 1 |
| 20 | The role of UV-B radiation in aquatic and terrestrial ecosystems—an experimental and functional analysis of the evolution of UV-absorbing compounds显示文摘 | J Rozema L.O Bj?rn J.F Bornman A Gaber??ik D.-P H?der T Tro?t M Germ M Klisch A Gr?niger R.P Sinha M Lebert Y.-Y He R Buffoni-Hall N.V.J de Bakker J van de Staaij B.B Meijkamp | 2002 | Journal of Photochemistry & Photobiology B: Biology2002，，1： | 1 |
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