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| 1 | Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function显示文摘Grafted embryonic central neural tissue pieces can recover function of hemisected spinal cord in neonatal rats and promote axonal growth in adults. However, spinal cord segments from adults have not been used as donor segments for allogeneic transplantation. Here, we utilized adult spinal cord tissue grafts(aSCGs) as donor constructs for repairing complete spinal cord injury(SCI). Moreover, to provide a favourable microenvironment for SCI treatment, a growth factor cocktail containing three growth factors(brain-derived neurotrophic factor, neurotrophin-3 and vascular endothelial growth factor), was applied to the aSCG transplants. We found that the locomotor function was significantly improved 12 weeks after transplantation of aSCGs into the spinal cord lesion site in adult rats. Transplantation of aSCGs combined with these growth factors enhanced neuron and oligodendrocyte survival and functional restoration. These encouraging results indicate that treatment of complete SCI by transplanting aSCGs, especially in the presence of growth factors, has a positive effect on motor functional recovery, and therefore could be considered as a possible therapeutic strategy for SCI. | He Shen Xi Chen Xing Li Ke Jia Zhifeng Xiao Jianwu Dai | 2019 | Science China(Life Sciences)2019，62，6： | 7 |
| 2 | Injectable collagen scaffold promotes swine myocardial infarction recovery by long-term local retention of transplanted human umbilical cord mesenchymal stem cells显示文摘Stem cell therapy is an attractive approach for recovery from myocardial infarction(MI)but faces the challenges of rapid diffusion and poor survival after transplantation.Here we developed an injectable collagen scaffold to promote the long-term retention of transplanted cells in chronic MI.Forty-five minipigs underwent left anterior descending artery(LAD)ligation and were equally divided into three groups 2 months later(collagen scaffold loading with human umbilical mesenchymal stem cell(hUMSC)group,hUMSC group,and placebo group(only phosphate-buffered saline(PBS)injection)).Immunofluorescence staining indicated that the retention of transplanted cells was promoted by the collagen scaffold.Echocardiography and cardiac magnetic resonance imaging(CMR)showed much higher left ventricular ejection fraction(LVEF)and lower infarct size percentage in the collagen/hUMSC group than in the hUMSC and placebo groups at 12 months after treatment.There were also higher densities of vWf-,α-sma-,and cTnT-positive cells in the infarct border zone in the collagen/cell group,as revealed by immunohistochemical analysis,suggesting better angiogenesis and more cardiomyocyte survival after MI.Thus,the injectable collagen scaffold was safe and effective on a large animal myocardial model,which is beneficial for constructing a favorable microenvironment for applying stem cells in clinical MI. | Qiang Wangn Xiaojun He Bin Wang Jun Pan Chunying Shi Jie Li Liudi Wang Yannan Zhao Jianwu Dai Dongjin Wang | 2021 | Science China(Life Sciences)2021，64，2： | 5 |
| 3 | Allotransplantation of adult spinal cord tissues after complete transected spinal cord injury: long-term survival and functional recovery in canines显示文摘Spinal cord injury(SCI), especially complete transected SCI, leads to loss of cells and extracellular matrix and functional impairments. In a previous study, we transplanted adult spinal cord tissues(aSCTs) to replace lost tissues and facilitate recovery in a rat SCI model. However, rodents display considerable differences from human patients in the scale, anatomy and functions of spinal cord systems, and responses after injury. Thus, use of a large animal SCI model is required to examine the repair efficiency of potential therapeutic approaches. In this study, we transplanted allogenic aSCTs from adult dogs to the lesion area of canines after complete transection of the thoracic spinal cord, and investigated the long-term cell survival and functional recovery. To enhance repair efficiency, a growth factor cocktail was added during aSCT transplantation, providing a favorable microenvironment. The results showed that transplantation of a SCTs, in particular with the addition of growth factors, significantly improves locomotor function restoration and increases the number of neurofilament-, microtubule-associated protein2-, 5-hydroxytryptamine-, choline acetyltransferase-and tyrosine hydroxylase-positive neurons in the lesion area at 6 months post-surgery. In addition, we demonstrated that donor neurons in a SCTs can survive for a long period after transplantation. This study showed for the first time that transplanting aSCTs combined with growth factor supplementation facilitates reconstruction of injured spinal cords, and consequently promotes long lasting motor function recovery in a large animal complete transected SCI model, and therefore could be considered as a possible therapeutic strategy in humans. | He Shen Shuyu Wu Xi Chen Bai Xu Dezun Ma Yannan Zhao Yan Zhuang Bing Chen Xianglin Hou Jiayin Li Yudong Cao Xianyong Fu Jun Tan Wen Yin Juan Li Li Meng Ya Shi Zhifeng Xiao Xingjun Jiang Jianwu Dai | 2020 | Science China(Life Sciences)2020，63，12： | 3 |
| 4 | Microvascular endothelial cells derived from spinal cord promote spinal cord injury repair显示文摘Neural regeneration after spinal cord injury (SCI) closely relates to the microvascular endothelial cell (MEC)- mediated neurovascular unit formation. However, the effects of central nerve system-derived MECs on neovascularization and neurogenesis, and potential signaling involved therein, are unclear. Here, we established a primary spinal cord-derived MECs (SCMECs) isolation with high cell yield and purity to describe the differences with brain-derived MECs (BMECs) and their therapeutic effects on SCI. Transcriptomics and proteomics revealed differentially expressed genes and proteins in SCMECs were involved in angiogenesis, immunity, metabolism, and cell adhesion molecular signaling was the only signaling pathway enriched of top 10 in differentially expressed genes and proteins KEGG analysis. SCMECs and BMECs could be induced angiogenesis by different stiffness stimulation of PEG hydrogels with elastic modulus 50-1650 Pa for SCMECs and 50-300 Pa for BMECs, respectively. Moreover, SCMECs and BMECs promoted spinal cord or brain-derived NSC (SNSC/BNSC) proliferation, migration, and differentiation at different levels. At certain dose, SCMECs in combination with the NeuroRegen scaffold, showed higher effectiveness in the promotion of vascular reconstruction. The potential underlying mechanism of this phenomenon may through VEGF/AKT/eNOS- signaling pathway, and consequently accelerated neuronal regeneration and functional recovery of SCI rats compared to BMECs. Our findings suggested a promising role of SCMECs in restoring vascularization and neural regeneration. | Zhifeng You Xu Gao Xinyi Kang Wen Yang Tiandi Xiong Yue Li Feng Wei Yan Zhuang Ting Zhang Yifu Sun He Shen Jianwu Dai | 2023 | Bioactive Materials2023，，11： | 2 |
| 5 | Recent developments in regenerative ophthalmology显示文摘Regenerative medicine(RM)is one of the most promising disciplines for advancements in modern medicine,and regenerative ophthalmology(RO)is one of the most active fields of regenerative medicine.This review aims to provide an overview of regenerative ophthalmology,including the range of tools and materials being used,and to describe its application in ophthalmologic subspecialties,with the exception of surgical implantation of artificial tissues or organs(e.g.,contact lens,artificial cornea,intraocular lens,artificial retina,and bionic eyes)due to space limitations.In addition,current challenges and limitations of regenerative ophthalmology are discussed and future directions are highlighted. | Ye Shen He Shen Dongyu Guo Xinghuai Sun Yuan Sun Nan Hong Xiawei Wang Chen Xie Yuan Zhao Qin He Le Jin Yingying Wen Bo Jiang Chenying Yu Miaomiao Zhu Feng Cai Jianwu Dai | 2020 | Science China(Life Sciences)2020，63，10： | 2 |
| 6 | l Benefits and costs to china of three different climate treaties显示文摘 | HAAKON V KRISTIN A HE Jianwu et a | 2009 | Resource and Energy Economics2009，31，： | 1 |
| 7 | Supply-side Economics in the People's Republic of China's Regional Context: A Quantitative Investigation of its VAT Reform 显示文摘 | Zhai Fan and Jianwu He | 2008 | Asian Economic Papers2008，7，2： | 1 |
| 8 | A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody显示文摘 | SHENG Jianwu HE Miao SHI Hanehang | 2007 | Anal Chim Acta2007，603，1： | 1 |
| 9 | Benefits and Costs to China of three Different Climate Treaties 显示文摘 | HAAKON V KRISTIN A HE Jianwu | 2009 | Resource and Energy Economics2009，31，： | 1 |
| 10 | A comprehensiveimmunoassay for the detection of microcystins in waters based onpolyclonal antibodies显示文摘 | SHENG Jianwu HE Miao SHI Hanchang | 2006 | Anal Chim Acta2006，572，： | 1 |
| 11 | A comprehensive immunoassay for the detection of microcystins in waters based on polyclonal antibodies显示文摘 | SHENG Jianwu HE Miao SHI Hanchang | 2006 | Anal Chim Acta2006，40，5： | 1 |
| 12 | Benefits and Costs to China of three Different Climate Treaties显示文摘 | HAAKON V KRISTIN A HE Jianwu | | 0，，： | 1 |
| 13 | Benefits and Costs to China of Three Different Climate Treaties 显示文摘 | Vennemo H Aunan K He Jianwu | 2009 | Resource and Energy Economics2009，，31： | 1 |
| 14 | Supply-side economics in the People' s Republic of China' s regional context: A quantitative investigation of its VAT reform显示文摘 | Fan Zhai He Jianwu | 2008 | Asian Economic Papers2008，7，2： | 1 |
| 15 | Using biochar for remediation of soils contaminated with heavy metals and organic pollutants显示文摘 | Xiaokai Zhang Hailong Wang Lizhi He Kouping Lu Ajit Sarmah Jianwu Li Nanthi S. Bolan Jianchuan Pei Huagang Huang | 2013 | Environmental Science and Pollution Research2013，，12： | 1 |
| 16 | A comprehensive immunoassay for the detection of microcystins in waters based on polyclonal antibodies显示文摘 | SHENG Jianwu HE Miao SHI Hanchang | 2006 | Anal Chim Acta2006，572，： | 1 |
| 17 | Rebalancing China''''s Economy-Modeling a Policy Package显示文摘 | Jianwu He Louis Kuijs | | 0，，07： | 1 |
| 18 | Mannose selection system used for cucumber transformation显示文摘 | Zhengquan He ZhenZhen Duan Wei Liang Faju Chen Wei Yao Hongwei Liang Chaoyin Yue Zongxiu Sun Fan Chen Jianwu Dai | 2006 | Plant Cell Reports2006，，9： | 1 |
| 19 | A highly specific immunoassay for microcystin-LR detection based on a monoclonal antibody显示文摘 | SHENG Jianwu HE Miao SHI Hanchang | 2007 | Anal Chim Acta2007，603，1： | 1 |
| 20 | Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay显示文摘Microcystins(MCs)are a group of closely related toxic cyclic heptapeptides produced by common cyanobacte-ria,which cause lots of accidents and threatens human health.In this paper,an indirect competitive enzyme-linked immu-nosorbent assay(ic-ELISA)was established and used to detect microcystin-LR(MC-LR)in drinking and surface waters.The concentration of coating antigen was 5 mg/mL,the dilution of monoclonal antibody MC10E7 was 1:3000,the dilution of enzyme tracer(goat anti-mouse IgG-peroxidase)was 1:3000,the standard concentration of MC-LR ranged from 0.001 mg/L to 30 mg/L,and o-phenylenediamine was used as substrate.The assay showed high relativity with high performance liquid chromatography(HPLC)with a correlation coefficient of more than 99%.The relative standard deviation was less than 10%,the detection limit was achieved down to 0.01 mg/L and up to 5.1 mg/L.The quantitative detection range was from 0.03 mg/L to 3 mg/L,and the antibody had high specificity for[4-arginine]microcystins.It performed well in spite of the influence of the real samples. | SHENG Jianwu HE Miao YU Shaoqing SHI Hanchang QIAN Yi | 2007 | Frontiers of Environmental Science & Engineering2007，1，3： | 1 |
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