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| 1 | HER2 in gastric cancer: Comparative analysis of three different antibodies using whole-tissue sections and tissue microarrays显示文摘AIM:To compare the performance of three commercially available anti-human epidermalgrowth factor receptor 2(HER2)antibodies in whole-tissue sections and tissue microarrays(TMAs)of a series of gastric tumors.METHODS:We present a comparative analysis of three anti-HER2 antibodies(HercepTest,4B5 and SP3)using TMA and whole-tissue sections prepared from the same paraffin blocks of 199 gastric adenocarcinomas operated upon between January 2004 and December2008 at a Brazilian cancer hospital.The data on the patients’age,sex,the anatomical location of the tumor and the Lauren’s histological classification were collected from clinical and pathological records.The immunohistochemical(IHC)results were examined by two pathologists and the cases were classified as positive(3+),equivocal(2+)and negative(0 or 1+),according to the criteria of the IHC scoring system of gastric cancer.TMAs and whole-tissue sections were evaluated separately and independently.All cases yielding discordant IHC results and/or scored as 2+were subjected to dual-color in situ hybridization in order to determine the final HER2 status.Besides determining the sensitivity and predictive value for HER2-positive status,we measured the accuracy of each antibody by calculating the area under the receiver operating characteristic(ROC)curve.The agreement between the results obtained using the TMAs and those obtained using the whole-tissue sections was assessed by means of Kappa coefficient.RESULTS:Intratumoral heterogeneity of HER2 expression was observed with all antibodies.HER2-positive expression(3+)in the whole-tissue sections was observed in 23 cases(11.6%)using the 4B5 antibody,in 18 cases(9.1%)using the SP3 antibody and in 10 cases(5.1%)using the HercepTest antibody.In the TMAs,11 positive cases(5.6%)were identified using SP3 antibody,9(4.6%)using the 4B5 antibody and 6(3%)using the HercepTest antibody.The sensitivity using whole-tissue sections and TMA,respectively,was 95.2%and 42.9%with 4B5,90.5%and 66.7%with SP3 and 47.6%and42.9%with HercepTest.The accuracy,calculated from the area under the ROC curve,using whole-tissue sections and TMA,respectively,was 0.91 and 0.79 by 4B5,0.86 and 0.80 by SP3 and 0.73 and 0.71 by HercepTest.The concordance of the results obtained using wholetissue sections and TMA was 97.4%(Kappa 0.75)using HercepTest,85.6%(Kappa 0.56)using SP3 and 84.1%(Kappa 0.38)using 4B5.CONCLUSION:The use of the 4B5 antibody on wholetissue sections was the most accurate IHC method for evaluating HER2 expression in gastric adenocarcinoma. | Lucas Faria Abraho-Machado Alexandre Andrade dos Anjos Jácome Durval Renato Wohnrath José Sebastio dos Santos Estela Cristina Carneseca José Humberto Tavares Guerreiro Fregnani Cristovam Scapulatempo-Neto | 2013 | World Journal of Gastroenterology2013，19，38： | 12 |
| 2 | Numerical Investigation of a Refractive Index SPR D-Type Optical Fiber Sensor Using COMSOL Multiphysics显示文摘 | D. F. SANTOS | 2013 | Photonic Sensors2013，3，1： | 8 |
| 3 | Prognostic significance of vascular endothelial growth factor polymorphisms in colorectal cancer patients显示文摘AIM To investigate the associations of the genetic polymor-phisms of vascular endothelial growth factor A(VEGF-A)-1498C>T and-634G>C, with the survival of patients with colorectal cancer(CRC). METHODS A prospective cohort consisting of 131 Brazilians patients consecutively operated on with a curative intention as a result of sporadic colorectal carcinoma was studied. DNA was extracted from peripheral blood and its amplification and allelic discrimination for each genetic polymorphism was performed using the technique of polymerase chain reaction(PCR) in real-time. The real-time PCR technique was used to identify the VEGF-A-1498C>T(rs833031) and-634G>C(rs2010963) polymorphisms. Genotyping was validated for VEGF-A-1498C>T polymorphism in 129 patients and for VEGF-A-634G>C polymorphism in 118 patients. The analysis of association between categorical variables was performed using logistic regression, survival by Kaplan-Meier method and multivariate analysis by the Cox regression method. RESULTS In the univariate analysis there was a significant association(OR = 0.32; P = 0.048) between genotype CC of the VEGF-A-1498C>T polymorphism and the presence of CRC liver metastasis. There was no association between VEGF-A-1498C>T polymorphism and VEGF-A-634G>C polymorphism with further clinical or anatomopathologic variables. The genotype CC of the VEGF-A-1498C>T polymorphism was significantly correlated with the 5-year survival(P = 0.032), but not significant difference(P = 0.27) was obtained with the VEGF-A-634G>C polymorphism with the 5-year survival in the univariate analysis. The genotype CT(HR = 2.79) and CC(HR = 4.67) of the polymorphism VEGF-A-1498C>T and the genotype CC(HR = 3.76) of the polymorphism VEGF-A-634C>G acted as an independent prognostic factor for the risk of death in CRC patients. CONCLUSION The CT and CC genotypes of the VEGF-A-1498C>T and the CC genotype of the VEGF-A-634C>G polymorphisms are prognostic factors of survival in Brazilians patients with sporadic colorectal carcinoma. | Gilmar Ferreira do Espírito Santo Bianca Borsatto Galera Elisabeth Carmen Duarte Elisabeth Suchi Chen Lenuce Azis Amilcar Sabino Damazo Gabriela Tognini Saba Flávia de Sousa Gehrke Ismael Dale Cotrim Guerreiro da Silva Jaques Waisberg | 2017 | World Journal of Gastrointestinal Oncology2017，9，2： | 4 |
| 4 | Innovation regional planning and latent dimensions: the case of the Algarve region显示文摘 | Hugo Pinto Jo?o Guerreiro | 2010 | The Annals of Regional Science2010，，2： | 2 |
| 5 | Seizure outcome and hippocampal atrophy in familial mesial temporal lobe epilepsy显示文摘 | Lopes-Cendes I Guerreiro CA | 2001 | Neurology2001，56，2： | 1 |
| 6 | Motor Neuron Syndromeas a New Phenotypic Manifestation of Mutation 9185T > C in GeneMTATP6 显示文摘 | Brum M Semedo C Guerreiro R | 2014 | Case Rep Neurol Med2014，2014，70： | 1 |
| 7 | Finite element analysis of a coupled thermally dependent viscosity flow problem 显示文摘 | Zhu J Loula A F D Karam F J Guerreiro J N C | 2007 | Comput Appl Math2007，26，1： | 1 |
| 8 | Determination of the antidepressant fluoxetine in human plasma by LC with UV detection 显示文摘 | MAYA M T DOMINGOS C R GUERREIRO M T | 2000 | J Pharma Biomed Anal2000，23，6： | 1 |
| 9 | The Developmental Competence of Oocyte, Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles 显示文摘 | Daniela Paes de Almeida Ferreira Braga Tatiana Carvalho de Souza Bonetti Ismael Dale Cotrim Guerreiro da Silva | 2013 | Int J Fertil Steril2013，6，4： | 1 |
| 10 | Protection of midbrain dopaminergic neurons by the end-product of purine metabolism uric acid: potentiation by low-level depolarization 显示文摘 | Guerreiro S Ponceau A Toulorge D | 2009 | J Neumchem2009，109，4： | 1 |
| 11 | A double-blind controlled clinical trial of oxcarbazepine versus phenytoin in children and adolescents with epilepsy显示文摘 | Guerreiro MM Vigonius U Pohlmann H | 1997 | Epilepsy Res1997，27，： | 1 |
| 12 | Aerenchyma formation and recovery from hypoxia of the flooded root system of nodulated soybean显示文摘 | Thomas A L Guerreiro S M C Sodek L | 2005 | Annals of Bota- ny2005，97，： | 1 |
| 13 | Transes- terification of soybean oil over sulfonic acid functionalised polymeric membranes 显示文摘 | Guerreiro L Castanheiro J E Fonseca I M | 2006 | Catalysis Today2006，118，12： | 1 |
| 14 | Aerenchyma forma- tion and recovery from hypoxia of the flooded root system of nodulated soybean 显示文摘 | Thomas A L Guerreiro S M C Soderk L | 2005 | Annals of Botany2005，96，： | 1 |
| 15 | Preliminary evaluation of military, commercial and novel skin decontamination products against a chemical wufare agent simulant (methyl salicylate ) 显示文摘 | Matar H Guerreiro A Piletsky SA | 2015 | Cutan Ocul Toxicol2015，4，： | 1 |
| 16 | A follow-up study of cog- nitive impairment due to inferior cap sulargenu infarction 显示文摘 | Madureira S Guerreiro M Ferro JM | 1999 | J Neurol1999，246，9： | 1 |
| 17 | Bone mineral density, vitamin D and anticonvulsant therapy显示文摘 | Filardi S Guerreiro CA Magna LA | 2000 | Arq Neuropsiquiatr2000，58，3： | 1 |
| 18 | Aerenchyma formation and recovery from hypoxia of the flooded root system of nodulated soybean显示文摘 | THOMAS A L GUERREIRO S M C SODEK L | 2005 | Annals of Botany2005，97，6： | 1 |
| 19 | The heritability and genetics of frontotemporal lobar degeneration 显示文摘 | Rohrer JD Guerreiro R Vandrovcova J | 2009 | Neurology2009，73，： | 1 |
| 20 | COL1A1 Sp1-binding site polymorphism as a risk factor for genital prolapse显示文摘 | Andrea Moura Rodrigues Manoel Jo?o Batista Castello Gir?o Ismael Dale Cotrim Guerreiro Silva Marair Gracio Ferreira Sartori Karina de Falco Martins Rodrigo de Aquino Castro | 2008 | International Urogynecology Journal2008，，11： | 1 |
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