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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Advantages of minimally- invasive reposition, retention, and Ilizarov-(hybrid)fixation for pilon-tibial-fractures fractures with particular emphasis on C2/C3 fractures 显示文摘 | Endres T Grass R Biewener A | 2004 | Unfallchirurg2004，107，4： | 1 |
| 3 | Renal glomerular lesions in unselected patients with cirrhosis undergoing orthotopic liver transplantation显示文摘 | Axelsen R A Crawford D H Endre Z H | | 0，，03： | 1 |
| 4 | Advantages of minimally-invasive reposition,retention,and Ilizarov-(hybrid) fixation for pilon-tibial-fractures with particular emphasis on C2/C3 fractures显示文摘 | Endres T Grass R Biewener A | 2004 | Unfallchirurg2004，107，4： | 1 |
| 5 | Electrodeposition of nanocrystalline metals and alloys from ionic liquids显示文摘 | Endres F Bukowski M Hempelmann R et at | 2003 | Angewandte Chemie International Edition2003，42，29： | 1 |
| 6 | Instrumented intervertebral or pos- terolateral fusion in elderly patients clinical results of a single cen-ter 显示文摘 | Endres S Aigner R Wilke A | 2011 | B MC Musculoskelet Disord2011，12，1： | 1 |
| 7 | Modem theories of entrepreneurship behaviors: A comparison and appraisal 显示文摘 | Endres A M Woods C R | 2006 | Small Business Economics2006，26，2： | 1 |
| 8 | Shedding of the amyloid precursor protein-like protein APLP2 by disintegvin-metalloproteinases 显示文摘 | Endres K Postina R Schroeder A | 2005 | FEBS J2005，272，22： | 1 |
| 9 | Early intervention with erythropoietin does not affect the outcome of acute kidney injury(the EARLYARF trial)显示文摘 | Endre ZH Walker R J Pickering JW | | 0，，： | 1 |
| 10 | A max flow approach to improved lower bounds for quadratic unconstrained binary optimization (QUBO) 显示文摘 | ENDRE B HAMMER P L SUN R | 2008 | Discrete Optimization2008，5，2： | 1 |
| 11 | The quest for high-conductance DNA显示文摘 | Endres R G Cox D L Singh R R P | 2004 | Rev Mod Phys2004，76，： | 1 |
| 12 | Blind equalization using the constant modulus criterion: areview 显示文摘 | Johnson C R Schniter P Endres T J | 1998 | Proceedings of the IEEE1998，86，10： | 1 |
| 13 | Blind equalization us- ingthe constant modulus criterion:A review显示文摘 | Johnson R Schniter P Endres T J | 1998 | Proceedings of IEEE1998，86，10： | 1 |
| 14 | Advantages of minimallyinvasive reposition,retention,and Ilizarrov-(hybrid) fixation for pilon-tibial-fractures fractures with particular emphasis on C2/C3 fractures显示文摘 | Endres T Grass R Biewener A | 2004 | Unfailchirurg2004，107，： | 1 |
| 15 | Blind equalization using the constant modulus criterion:a review显示文摘 | JOHNSON R J SCHNITER P ENDRES T J | 1998 | Proceedings of the IEEE1998，86，10： | 1 |
| 16 | The effect of time-to-surgery on outcome in elderly patients with proximal femoral fractures 显示文摘 | Smektala R Endres HG Dasch B | 2008 | BMC Musculoskelet Disord2008，9，： | 1 |
| 17 | Incorporating pore geometry and fluidpressure communication into modeling the elastic behavior ofporous rocks显示文摘 | Endres A L Knight R J | 1997 | Geophysics1997，62，1： | 1 |
| 18 | Electrodeposition of nanocrystalline metals and alloys from ionic liquids显示文摘 | Endres F Bukowski M Hempelmann R | 2003 | Angewandte Chemie International Edtion2003，42，29： | 1 |
| 19 | Clinical results after DFNOsteosynthesis 显示文摘 | Grass R Biewener A Endres T | 2002 | Unfallchirurg2002，105，7： | 1 |
| 20 | Ionic-liquidmaterials for the electrochemical challenges of the future显示文摘 | ARMAND M ENDRES F MACFARLANE D R | 2009 | NatureMater2009，8，： | 1 |
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