|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Cloning of first abc transporter encoding gene from Trichoderma spp. and its expression during stress and mycoparasitism显示文摘Trichoderma in its natural environment competes for nutrient uptake and is required to protect itself from adverse natural toxic compounds, such as those produced by plants and other microbes in the soil community, or synthetic toxic compounds released human activity. One of the most important metabolic pathways for drug resistance and substrate uptake, both in prokaryotes and eukaryotes, is ATP dependent. The role of ABC transporter proteins in the biology of Trichoderma is still not known. We present the cloning of the first four ABC transporter genes (TABC1, TABC2, TABC3, TABC4 ) in Trichoderma, and in particular T. atroviride P1, and the characterization of TABC2 The complete sequence of this gene is 6535 bp, which includes a promoter of 1624 bp, a terminator of 642 bp and a coding region of 4264 bp. The promoter contains many of the potential transcription factor binding sites found in the 5’ upstream region of the ech42 gene of T. atroviride P1. These included: heat shock factors (HSF), a nitrogen-regulating factor (Nit-2), a stress-response element (STRE), a GCR1 elements, and a Cre BP1 motif. Northern analysis and RT-PCR demonstrated that TABC2 is highly expressed when Trichoderma is subjected to nitrogen starvation, grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum, or when N-acetylglucosamine is added to the substrate. TABC2 appears to be co-regulated with some CWDE-encoding genes, suggesting that this is the first ABC transporter encoding gene involved in mycoparasitic events. It’s role in the interaction of Trichoderma with fungal hosts or plants is being investigated by targeted gene disruption and overexpression. | Lanzuise S Ruocco M Scala V Catapano L Woo S Ciliento R Ferraioli S Soriente I Vinale F Scala F Del Sorbo G Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 3 | S-adenosyl-methionine decreases ethanol-induced apoptosis in primary hepatocyte cultures by a c-Jun N-terminal kinase activity-independent mechanism显示文摘AIM:To determine the role of c-Jun N-terminal kinase(JNK)activity in ethanol-induced apoptosis and themodulation of this signaling cascade by S-Adenosyl-methionine(AdoMet).METHODS:Primary hepatocyte cultures werepretreated with 100 μmol/L SP600125,a selective JNKinhibitor,1 mL/L DMSO or 4 mmol/L AdoMet and thenexposed to 100 mmo/L ethanol.Hepatocyte apoptosiswas determined by the TUNEL and DNA ladder assays.JNK activity and its inhibition by SP600125 and AdoMetwere determined by Western blot analysis of c-junphosphorylation and Bid fragmentation.SP600125 andAdoMet effects on the apoptotic signaling pathway weredetermined by Western blot analysis of cytochrome crelease and pro-caspase 3 fragmentation.The AdoMeteffect on glutathione levels was measured by Ellman'smethod and reactive oxygen species(ROS)generationby cell cytometry.RESULTS:The exposure of hepatocytes to ethanolinduced JNK activation,c-jun phosphorylation,Bidfragmentation,cytochrome c release and pro-caspase 3cleavage;these effects were diminished by SP600125,and caused a significant decrease in ethanol-inducedapoptosis(P<0.05).AdoMet exerted an antioxidanteffect maintaining glutathione levels and decreasing ROSgeneration,without a significant effect on JNK activity,and prevented cytochrome c release and pro-caspase 3cleavage. CONCLUSION:The JNK signaling cascade is a keycomponent of the proapoptotic signaling pathwayinduced by ethanol.JNK activation may be independentfrom ROS generation,since AdoMet which exertedantioxidant properties did not have a significant effect onJNK activity.JNK pathway modulator agents and AdoMetmay be components of promising therapies for alcoholicliver disease(ALD)treatment. | Maria del Pilar Cabrales-Romero Lucrecia Márquez-Rosado Samia FatteI-Fazenda Cristina Trejo-Solis Evelia Arce-Popoca Leticia Alemán-Lazarini Saúl Villa-Trevineo | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 4 | Localization of manganese-superoxide dismutase in peroxisomes isolated from Pisum sativum L显示文摘 | Sandalio L M Palma J M del Río L A | 1987 | Plant Science1987，51，： | 1 |
| 5 | Determination of rotenone residues in raw honey by solid-phase extraction and high-performance liquid chromatography 显示文摘 | Jimenez J J Bernal J L Del M J | 2000 | J Chromatogr A2000，871，12： | 1 |
| 6 | Stress proteins induced by arsenic 显示文摘 | Del Razo L M QuintaniUa - Vega B Brambila - Colom- bres E | 2001 | Toxicology and Applied Pharmacology2001，177，2： | 1 |
| 7 | Effectiveness of the association of 2 probiotic strains formulated in a slow release vaginalproduct,in women affected by vulvovaginalcandidiasis: a pilot study显示文摘 | Vicariotto F Del Piano M Mogna L | 2012 | J Clin Gastroenterol2012，，： | 1 |
| 8 | Effect of wheat cultivar and enzyme addition to broiler chicken diets on nutrient digestibility,performance,and apparent metabolizable energy content显示文摘 | Gutierrez del Alamo A Verstegen M W Den Hartog L A | 2008 | Poult Sci2008，87，4： | 1 |
| 9 | Gene transfer in vivo with DNA-liposome complexes: safety and acute toxicity in mice显示文摘 | Stewart M J Plautz G E Del Buono L | 1992 | Hum Gene Ther1992，3，3： | 1 |
| 10 | Transient hypoxia ischemia in rats: changes in diffusion sensitive MR imaging findings, extracellular space, and Na + -K + -adenosine triphosphatasc and cytochrome oxidase activity 显示文摘 | Qiao M Malisza K L Del Bigio M R | 2002 | Radiology2002，223，： | 1 |
| 11 | Partial characterization of banana starches oxidized by different levels of sodium hypoehlorite显示文摘 | Sanchez-Rivera M M Garcla-Suarez F J L Velazquez del Valle M | 2005 | Carbohydrate Poly mers2005，62，1： | 1 |
| 12 | ARDSNet lower tidal volume ventilatory strategy may generate intrinsic positive end-expiratory pressure in patients with acute respiratory distress syndrome显示文摘 | de Durante G del Turco M Rnstichini L | 2002 | Am J Respir Crit Care Med2002，165，9： | 1 |
| 13 | MALDI-TOF MS proteomic phenotyping of filamentous and other fungi from clinical origin 显示文摘 | Del Chierico F Masotti A Onori M Fiscarelli E Mancinelli L Ricciotti G | 2012 | Journal of proteomics2012，75，11： | 1 |
| 14 | Endoglin:an accessory component of the TGFβ-binding receptor complex with diagnostic,prognostic,and bioimmunotherapeutic potential in human malignancies显示文摘 | Fonsatti E Del Vecchio L Altomonte M | 2001 | J Cell Physiol2001，188，： | 1 |
| 15 | Digestive enzymes in juvenile green abalone,Haliotis fulgens, fed natural food显示文摘 | Garcia-Carrefio F L Navarrete del Toro M A Serviere-Zaragoza E | 2003 | Comp Biochem Physiol2003，134，： | 1 |
| 16 | Natural senescence of pea leaves(an activated-oxygen-mediated function for peroxisomes) 显示文摘 | Pastori G M Del Rio L A | 1997 | Plant Physiology1997，113，2： | 1 |
| 17 | Comparative toxicity of trivalent and pentavalent inorganic and methylated arsenicals in rat and human cells 显示文摘 | Styblo M Del Razo L M Vega L | 2000 | Ar- chives of Toxicology2000，74，6： | 1 |
| 18 | Endoglin: An accessory component of the TGF-b-binding receptor-complex with diagnostic, prognostic, and bioimmunotherapeutic potential in human malignancies 显示文摘 | Fonsatti E Del Vecchio L Altomonte M | 2001 | J Cell Physio12001，188，1： | 1 |
| 19 | Color changes dur- ing storage of honeys in relation to their composition and initial color显示文摘 | Pereyra Gonzales A Burin L and Del Pilar Buera M | 1999 | Food Research International1999，32，： | 1 |
| 20 | Biologics in children's autoimmune disorders:efficacy and safety显示文摘 | Breda L Del Torto M De Sanctis S | | 0，，02： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员