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| 1 | 抗体/腺病毒复合物为载体的基因定位递送显示文摘临床基因治疗方案中基因载体在疾病部位的靶向递送仍是急待解决的问题。本研究将腺病毒与一种生物素化的特异性抗腺病毒六邻体的多克隆IgG结合,固定于结合了亲和素的胶原蛋白膜上,成功获得腺病毒载体基因靶向定位递送体系。体外稳定性研究结果表明该体系中病毒载体可有效保持活性。通过这种特异性抗体偶联方式,将携带单纯疱疹胸苷激酶(HSVtk)编码基因片断的腺病毒结合在胶原膜上转染大鼠平滑肌细胞(A10),加入更昔洛韦(ganciclovir)后,只有生长在胶原膜上及膜邻近50μm内的细胞被杀死。在使用非特异性抗体的对照实验中,整个培养基范围内的细胞几乎全被杀死。以绿色荧光蛋白(GFP)为报告基因对猪的心肌进行转基因实验,结果显示注射特异性抗体偶联病毒的胶原凝胶比直接注射病毒悬液获得更高效的心室基因表达。所有研究结果表明,通过生物素和特异性抗体使病毒载体固定在胶原蛋白基质上,可达到有效的局部定位基因表达,避免向非病灶部位的扩散,是基因治疗中一种极具发展潜力的载体定位递送方法。 | 宋存先 王满燕 RJ Levy S Tallapragada S DeFelice J T Hinson | 2005 | 中国生物医学工程学报2005，24，5： | 2 |
| 2 | Inactivation of L-type Ca channels in embryonic chick ventricle cells: dependence on the cytoskeletal agents colchicines and taxol显示文摘 | Galli A DeFelice LJ | 1994 | Biophys1994，67，： | 1 |
| 3 | Endothelial injury in the initiation and progression of vascular disorders显示文摘 | DeFelice AF | 2007 | Vascul Pharmacol2007，46，： | 1 |
| 4 | Common apolipoprotein A-Ⅳ variants are associated with differences in body mass index levels and percentage body fat显示文摘 | Lovejoy JC DeFelice SM | 2000 | Int J Obes Rslat Metab Disord2000，24，8： | 1 |
| 5 | Yellow nutsedge Cyperusescu lentus L-Snackfood of the gods显示文摘 | Defelice M S | 2002 | Weed Technology2002，16，： | 1 |
| 6 | Phase II trial of thali- domide and daily oral dexamethasone for treatment of hormone refractory prostate cancer progressing after chemotherapy显示文摘 | Remero S Stantou G DeFelice J | 2007 | Urol Oncol2007，25，4： | 1 |
| 7 | Molecular strueture of ilvH and its evolutionary relationship to ilvG in Esehierchia coli K-12显示文摘 | Squires C H Defelice M | 1983 | NueleieAeids Research1983，，11： | 1 |
| 8 | Stochastic resonance in biological systems显示文摘 | Petracchi D Gebeshuber I C Defelice L J | 2000 | Chaos & Fractals2000，11，22： | 1 |
| 9 | Thyroidnuclearfactor-1containsahomeodomainanddisplaysanovelDNA bindingspecificity显示文摘 | GuzziS Price M DeFelice M etal | 1990 | DMBOJ1990，9，： | 1 |
| 10 | Cardi- ac autotransplant for surgical treatment of a malignant he-oplasm 显示文摘 | Reardon M J DeFelice C A Sheinbaum R | 1999 | Ann Thorac Surg1999，67，6： | 1 |
| 11 | Protec- tion of synapses against Alzheimer' s-linked toxins: Insulin signaling prevents the pathogenic binding of A beta oli- gomers 显示文摘 | DEFELICE FG VIEIRA MN BOMFIM TR | 2009 | PNAS2009，106，6： | 1 |
| 12 | Di- 2- ethylhexyl phthalate and endocrine disruption: A review显示文摘 | Latinl G Verrotti A Defelice C | 2004 | Current Drug Targets - Immune Endocrine & Metabolic Disorders2004，4，1： | 1 |
| 13 | Endothelial injury in the in- itiation and progression of vascular disorders 显示文摘 | Tesfamariam B DeFelice AF | 2007 | VascuI Pharmacol2007，46，4： | 1 |
| 14 | Endothelial injury in the initiation and progression of vascular disorders显示文摘 | Tesfamariam B DeFelice AF | 2007 | Vascul Pharmacol2007，46，4： | 1 |
| 15 | Gene delivery from a DNA controlled-release stent in porcine coronary arteries显示文摘 | Klugherz BD Jones PL Cui X Chen W Meneveau NF DeFelice S | 2000 | Nature Biotechnol2000，18，11： | 1 |
| 16 | Gene delivery to pig coronary arteries from stents carrying antibody-tethered adenovirus 显示文摘 | Klugherz BD Song C DeFelice S | 2002 | Hum Gene Ther2002，13，3： | 1 |
| 17 | Stochastic resonance in biological systems显示文摘 | Gebeshuber I C DeFelice L J | 2000 | Chaos Solitons & Fractals2000，11，： | 1 |
| 18 | Acute hepatic failure in a child treated with lamotrigine 显示文摘 | Devivo D Defelice AR | 1998 | Pediatr Neurology1998，18，13： | 1 |
| 19 | In utero exposure to di - (2 - ethylhexyl) phthalate and duration of human pregnancy 显示文摘 | Latini G DeFelice C Presta G | 2003 | EnvironHealth Perspect2003，111，14： | 1 |
| 20 | Di-2-ethylhexyl phthalate and endocrine Disruption:a review显示文摘 | Latini G Verrotti A DeFelice C | 2004 | Curr Drug Targets Immune Endocr Metabol Disord2004，4，1： | 1 |
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