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| 1 | Solid-state fermentation of corn-soybean meal mixed feed with Bacillus subtilis and Enterococcus faecium for degrading antinutritional factors and enhancing nutritional value显示文摘Background: Corn and soybean meal(SBM) are two of the most common feed ingredients used in pig feeds.However, a variety of antinutritional factors(ANFs) present in corn and SBM can interfere with the bioavailability of nutrients and have negative health effects on the pigs. In the present study, two-stage fermentation using Bacillus subtilis followed by Enterococcus faecium was carried out to degrade ANFs and improve the nutritional quality of corn and SBM mixed feed. Furthermore, the microbial composition and in vitro nutrient digestibility of inoculated mixed feed were determined and compared those of the uninoculated controls.Results: During the fermentation process, B. subtilis and lactic acid bacteria(LAB) were the main dominant bacteria in the solid-state fermented inoculated feed, and fermentation produced a large amount of lactic acid(170 mmo L/kg),which resulted in a lower pH(5.0 vs. 6.4) than the fermented uninoculated feed. The amounts of soybean antigenic proteins(β-conglycinin and glycinin) in mixed feed were significantly decreased after first-stage fermentation with B. subtilis. Inoculated mixed feed following two-stage fermentation contained greater concentratioin of crude protein(CP), ash and total phosphorus(P) compared to uninoculated feed, whereas the concentrations of neutral detergent fiber(NDF), hemicellulose and phytate P in fermendted inoculated feed declined(P < 0.05) by 38%, 53%, and 46%,respectively. Notably, the content of trichloroacetic acid soluble protein(TCA-SP), particularly that of small peptides and free amino acids(AA), increased 6.5 fold following two-stage fermentation. There was no difference in the total AA content between fermented inoculated and uninoculated feed. However, aromatic AAs(Phe and Tyr) and Lys in inoculated feed increased, and some polar AAs, including Arg, Asp, and Glu, decreased compared with the uninoculated feed. In vitro dry matter and CP digestibility of inoculated feed improved(P < 0.05) compared with the uninoculated feed.Conclusions: Our results suggest that two-stage fermentation using B. subtilis followed by E. faecium is an effective approach to improve the quality of corn-soybean meal mixed feed. | Changyou Shi Yu Zhang Zeqing Lu Yizhen Wang | 2017 | Journal of Animal Science and Biotechnology2017，8，4： | 44 |
| 2 | Solid state fermentation of rapeseed cake with Aspergillus niger for degrading glucosinolates and upgrading nutritional value显示文摘Background:Rapeseed cake is a good source of protein for animal feed but its utilization is limited due to the presence of anti-nutritional substances,such as glucosinolates(GIs),phytic acid,tannins etc.In the present study,a solid state fermentation(SSF) using Aspergillus niger was carried out with the purpose of degrading glucosinolates and improving the nutritional quality of rapeseed cake(RSC).The effects of medium composition and incubation conditions on the GIs content in fermented rapeseed cake(FRSC) were investigated,and chemical composition and amino acid in vitro digestibility of RSC substrate fermented under optimal conditions were determined.Results:After 72 h of incubation at 34℃,a 76.89%decrease in GIs of RSC was obtained in solid medium containing 70%RSC,30%wheat bran at optimal moisture content 60%(w/w).Compared to unfermented RSC,trichloroacetic acid soluble protein(TCA-SP),crude protein and ether extract contents of the FRSC were increased(P< 0.05) 103.71,23.02 and 23.54%,respectively.As expected,the contents of NDF and phytic acid declined(P< 0.05) by 9.12 and 44.60%,respectively.Total amino acids(TAA) and essential amino acids(EAA) contents as well as AA in vitro digestibility of FRSC were improved significantly(P< 0.05).Moreover,the enzyme activity of endoglucanase,xylanase,acid protease and phytase were increased(P<0.05) during SSF.Conclusions:Our results indicate that the solid state fermentation offers an effective approach to improving the quality of proteins sources such as rapeseed cake. | Changyou Shi Jun He Jie Yu Bing Yu Zhiqing Huang Xiangbing Mao Ping Zheng Daiwen Chen | 2015 | Journal of Animal Science and Biotechnology2015，6，3： | 32 |
| 3 | PIKA as an Adjuvant Enhances Specific Humoral and Cellular Immune Responses Following the Vaccination of Mice with HBsAg plus PIKA显示文摘An adjuvant is usually used to enhance the immune response induced by vaccines. The choice of adjuvant or immune enhancer determines the effectiveness of the immune response. Currently, aluminium (Alum, a generic term for salts of aluminium) is the only FDA-approved adjuvant. Alum predominantly induces the differentiation of Th2 cells and thus mediates an antibody immune response. Therefore, there is an urgent need for new adjuvants that enhance not only humoral but also cellular immune responses. In the present study, we demonstrates that PIKA (a stabilized dsRNA) as an adjuvant directly induces the activation and the proliferation of both B and NK cells in vitro. Injection of PIKA into mice results in the production of cytokines in vivo. In addition, the study demonstrates that PIKA promotes the maturation of bone marrow-derived dendritic cells (BMDCs) including up-regulation of the co-stimulatory molecules CD80, CD86 and CD40, and the induction of cytokines such as IL-12p70, IL-12p40 and IL-6. Importantly, after immunization of mice with HBsAg plus PIKA, the presence of PIKA enhances the titers of HBsAg-specific IgG and HBsAg-specific IFN-γ production. These results demonstrate that PIKA as an adjuvant can promote both humoral and cellular immune responses. These might have an implication in applying PIKA as an adjuvant to be used in the design and development of both therapeutic and preventive vaccines, and used in the clinical study. | Erxia Shen Li Li Lietao Li Lianqiang Feng Lin Lu Ziliang Yao Haixiang Lin Changyou Wu | 2007 | Cellular & Molecular Immunology2007，4，2： | 13 |
| 4 | Rock-breaking mechanism and experimental analysis of confined blasting of borehole surrounding rock显示文摘The rock-breaking mechanism and effect of confined blasting were analysed by blasting and impact dynamic mechanics, fluid dynamic mechanics, fracture mechanics as well as blasting experiment. The results showed that the fracturing of surrounding rock in confined blasting condition is the result of coaction of rock pre-cracking by shock wave and stress wave and the continuing expanding crackenhancement of confined medium, and the model of crack development of borehole surrounding rock in confined blasting condition was established. This study acquired the damage range of surrounding rock under the action of shock wave and stress wave, as well as the crack development characteristics of surrounding rock after the wedge-in confined medium into the crack space. Deep-hole confined blasting experiment on large rock showed that the high-efficient utilisation of in-hole explosive was achieved and the safety of rock blasting operation was ensured. Safe static rock-breaking under the action of high-efficient explosive blasting was achieved as well as the unification of super dynamic load of explosive blasting and static rock-breaking of water medium. | Liu Changyou Yang Jingxuan Yu Bin | 2017 | International Journal of Mining Science and Technology2017，27，5： | 12 |
| 5 | The migration of total dissolved solids during natural freezing process in Ulansuhai Lake显示文摘High total dissolved solids (TDS) content is one of the most important pollution contributors in lakes in arid and semiarid areas.Ulansuhai Lake,located in Urad Qianqi,Inner Mongolia,China,was selected as the object of study.Temperatures and TDS contents of both ice and under-ice water were collected together with corresponding ice thickness.TDS profiles were drawn to show the distribution of TDS and to describe TDS migration.The results showed that about 80% (that is 3.602×10 8 kg) of TDS migrated from ice to water during the whole growth period of ice.Within ice layer,TDS migration only occurred during initial ice-on period,and then perished.The TDS in ice decreased with increasing ice thickness,following a negative exponential-like trend.Within under-ice water,the TDS migrated from ice-water interface to the entire water column under the effect of concentration gradient until the water TDS content was uniform.In winter,6.044×10 7 kg (16.78% of total TDS) TDS migrated from water to sediment,which indicated that winter is the best time for dredging sediment.The migration effect gives rise to TDS concentration in under-ice water and sediment that is likely to affect ecosystem and water quality of the Yellow River.The trend of transfer flux of ice-water and water-sediment interfaces is similar to that of ice growth rate,which reveals that ice growth rate is one of the determinants of TDS migration.The process and mechanism of TDS migration can be referenced by research on other lakes with similar TDS content in cold and arid areas. | Yan ZHANG ChangYou LI XiaoHong SHI Chao LI | 2012 | Journal of Arid Land2012，4，1： | 12 |
| 6 | On the Uniqueness of Heat Flow of Harmonic Maps and Hydrodynamic Flow of Nematic Liquid Crystals显示文摘For any n-dimensional compact Riemannian manifold (M,g) without boundary and another compact Riemannian manifold (N,h), the authors establish the uniqueness of the heat flow of harmonic maps from M to N in the class C([0,T),W1,n). For the hydrodynamic flow (u,d) of nematic liquid crystals in dimensions n = 2 or 3, it is shown that the uniqueness holds for the class of weak solutions provided either (i) for n = 2, u ∈ Lt∞ L2x∩L2tHx1, ▽P∈ Lt4/3 Lx4/3 , and ▽d∈ L∞t Lx2∩Lt2Hx2; or (ii) for n = 3, u ∈ Lt∞ Lx2∩L2tHx1∩ C([0,T),Ln), P ∈ Ltn/2 Lxn/2 , and ▽d∈ L2tLx2 ∩ C([0,T),Ln). This answers affirmatively the uniqueness question posed by Lin-Lin-Wang. The proofs are very elementary. | Fanghua LIN Changyou WANG | 2010 | Chinese Annals of Mathematics,Series B2010，31，6： | 11 |
| 7 | Restoration of rat calvarial defects by poly(lactide-co-glycolide)/hydroxyapatite scaffolds loaded with bone mesenchymal stem cells and DNA complexes显示文摘A composite construct comprising of a bone mesenchymal stem cell (BMSC) sheet, plasmid DNA, encoding human bone morphogenic protein-2 (hBMP-2), and poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) sponge was designed and employed in the restoration of rat calvarial defects. To improve gene transfection efficiency, a cationic chitosan derivative, N,N,N,-trimethyl chitosan chloride (TMC), was employed as the vector. The TMC/DNA complexes had a transfection efficiency of 13% in rat BMSCs, resulting in heterogeneous hBMP-2 expression in a 10-d culture period in vitro. In vivo culture of the composite constructs was performed by implantation into rat full-thickness calvarial defects, using constructs lacking pDNA-hBMP-2 or BMSC sheets as controls. Significantly higher heterogeneous expression of hBMP-2 was detected in vivo at 2 weeks for the cell sheet/DNA complex/scaffold constructs, compared with the constructs lacking pDNA-hBMP-2 or BMSC sheets. New bone formation was evident as early as 4 weeks in the experimental constructs. At 8 weeks, partial bridging of calvarial defects was observed in the experimental constructs, which was significantly better than the constructs lacking pDNA-hBMP-2 or BMSC sheets. Therefore, the combination of the PLGA/HA scaffold with BMSC sheets and gene therapy vectors is effective at enhancing bone formation. | LI Dan WANG Wei GUO Rui QI YiYing GOU ZhongRu GAO ChangYou | 2012 | Chinese Science Bulletin2012，57，5： | 9 |
| 8 | Adaptable hydrogel with reversible linkages for regenerative medicine:Dynamic mechanical microenvironment for cells显示文摘Hydrogels are three-dimensional platforms that serve as substitutes for native extracellular matrix.These materials are starting to play important roles in regenerative medicine because of their similarities to native matrix in water content and flexibility.It would be very advantagoues for researchers to be able to regulate cell behavior and fate with specific hydrogels that have tunable mechanical properties as biophysical cues.Recent developments in dynamic chemistry have yielded designs of adaptable hydrogels that mimic dynamic nature of extracellular matrix.The current review provides a comprehensive overview for adaptable hydrogel in regenerative medicine as follows.First,we outline strategies to design adaptable hydrogel network with reversible linkages according to previous findings in supramolecular chemistry and dynamic covalent chemistry.Next,we describe the mechanism of dynamic mechanical microenvironment influence cell behaviors and fate,including how stress relaxation influences on cell behavior and how mechanosignals regulate matrix remodeling.Finally,we highlight techniques such as bioprinting which utilize adaptable hydrogel in regenerative medicine.We conclude by discussing the limitations and challenges for adaptable hydrogel,and we present perspectives for future studies. | Zongrui Tong Lulu Jin Joaquim Miguel Oliveira Rui LReis Qi Zhong Zhengwei Mao Changyou Gao | 2021 | Bioactive Materials2021，6，5： | 8 |
| 9 | Arch structure effect of the coal gangue flow of the fully mechanized caving in special thick coal seam and its impact on the loss of top coal显示文摘Based on the characteristics of the top coal thickness of the fully mechanized caving in special thick coal seam,the long distance of coal gangue caving,as well as the different sizes of the coal gangue broken fragment dimension and spatial variation of drop flow,this paper uses laboratory dispersion simulation experiment and theoretical analysis to study the arch structure effect and its influence rule on the top coal loss in the process of coal gangue flow.Research shows that in the process of coal gangue flow,arch structure can be formed in three types:the lower arch structure,middle arch structure,and upper arch structure.Moreover,the arch structure has the characteristics of dynamic random arch,the formation probability of dynamic random arch with different layers is not the same,dynamic random arch caused the reduction of the top coal fluency;analyzing the dynamic random arch formation mechanism,influencing factors,and the conditions of instability;the formation probability of the lower arch structure is the highest,the whole coal arch and the coal gangue arch structure has the greatest impact on top coal loss.Therefore,to prevent or reduce the formation of lower whole coal arch structure,the lower coal gangue arch structure and the middle whole coal arch structure is the key to reduce the top coal loss.The research conclusion provides theoretical basis for the further improvement of the top coal recovery rate of the fully mechanized caving in extra thick coal seam. | Zhang Ningbo Liu Changyou | 2016 | International Journal of Mining Science and Technology2016，26，4： | 8 |
| 10 | Rational cutting height for large cutting height fully mechanized top-coal caving显示文摘Large cutting height fully mechanized top-coal caving is a new mining method that improves recovery ratio and single-pass production. It also allows safe and efficient mining. A rational cutting height is one key parameter of this technique. Numerical simulation and a granular-media model experiment were used to analyze the effect of cutting height on the rock pressure of a fully mechanized top-coal caving work face. The recovery ratio was also studied. As the cutting height increases the top-coal thickness is reduced. Changing the ratio of cutting to drawing height intensifies the face pressure and the top-coal shattering. A maximum cutting height exists under a given set of conditions due to issues with surrounding rock-mass control. An increase in cutting height makes the top-coal cave better and the recovery ratio when drawing top-coal is then improved. A method of adjusting the face rock pressure is presented. Changing the cutting to drawing height ratio is the technique used to control face rock pressure. The recovery ratio when cutting coal exceeds that when caving top-coal so the face recovery ratio may be improved by over sizing the cutting height and increasing the top-coal drawing ratio. An optimum ratio of cutting to drawing height exists that maximizes the face recovery ratio. A rational cutting height is determined by comprehensively considering the surrounding rock-mass control and the recovery ratio. At the same time increasing the cutting height can improve single pass mining during fully mechanized top-coal caving. | Huang Bingxiang Li Hongtao Liu Changyou Xing Shijun Xue Weichao | 2011 | Mining Science and Technology2011，21，3： | 7 |
| 11 | Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ显示文摘Cell migration in scaffolds plays a crucial role in tissue regeneration,which can better mimic cell behaviors in vivo.In this study,a novel model has been proposed on controlling 3D cell migration in porous collagen-chitosan scaffolds with various pore structures under the stimulation of inflammatory cells to mimic the angiogenesis process.Endothelial cells(ECs)cultured atop the scaffolds in the Transwell molds which were placed into a well of a 24-well culture plate were promoted to migrate into the scaffolds by chemoattractants such as vascular endothelial growth factor(VEGF)and tumor necrosis factor-alpha(TNF-α)secreted by the pro-inflammatory macrophages incubated in the well culture plate.The phenotype of macrophages was mediated by 50 ng/ml interferongamma(IFN-c)and different concentrations of lipopolysaccharide(LPS,150–300 ng/ml).The cell migration depth had a positive correlation with LPS concentration,and thereby the TNF-a concentration.The ECs migrated easier to a deeper zone of the scaffolds prepared at10C(187 lm in pore diameter)than that at20C(108 lm in pore diameter)as well.The method provides a useful strategy to study the 3D cell migration,and is helpful to reveal the vascularization process during wound healing in the long run. | Xuguang Li Yuankun Dai Tao Shen Changyou Gao | 2017 | Regenerative Biomaterials2017，4，3： | 7 |
| 12 | Construction and characteristics of a transformed lepidopteran cell clone expressing baculovirus p35显示文摘A transformed cell line was constructed fromMythimna separata cells Ms7311 by lipofection methodTMs7311 cells were generated using a double selection tech-nique involving a selection in the antibiotic Zeocin, followedby a second round of selection by exhibiting cell characteri-zation. A cell clone expressing p35 was obtained with highlevel of AcMNPV and recombinant proteins. Compared withwild type Ms7311 cells, the cell clone showed increased resis-tance to Actinamycin D-induced apoptosis and a profoundresistance to nutrient development (PBS). When the cellclone was infected with recombinant baculoviruses express-ing secreted alkaline phosphatase (SEAP) and β-galactosi-dase, expression of the recombinant proteins from TMs7311cells exceeded that from parental Ms7311 cells. Production ofbudded virus and occlusion body was significantly higherthan that from parental cells Ms7311. | ZHENG Gulling LI Changyou LI Guoxun WANG Ping Robert R. Granados | 2005 | Chinese Science Bulletin2005，50，23： | 6 |
| 13 | Similarity simulation of bolt support in a coal roadway in a tectonic stress field显示文摘In order to study the mechanism of bolt support and the behavior of strata in a coal roadway under tectonic stress,deformation and destruction of a roof,floor and sides were studied using an experiment in similarity simulation.We also studied the mechanism and types of bolt support functions in the coal roadway.The results show that with an increase in horizontal tectonic stress,the strata in the roof and floor of the roadway gradually separate and become shear failure areas.Coal in side walls moves,but its integrity remains intact.Side bolts are mainly affected by tension and roof bolts by the effect of shear. | LU Yan LIU Changyou | 2010 | Mining Science and Technology2010，20，5： | 6 |
| 14 | Effects of gully terrain on stress field distribution and ground pressure behavior in shallow seam mining显示文摘This study proposes a novel approach to study stress field distribution and overlying ground pressure behavior in shallow seam mining in gully terrain.This approach combines numerical simulations and field tests based on the conditions of gully terrain in the Chuancao Gedan Mine.The effects of gully terrain on the in situ stress field of coal beds can be identified by the ratio of self-weight stress to vertical stress(η) at the location corresponding to the maximum vertical stress.Based on the function η =j(h),the effect of gully terrain on the stress field of overlying strata of the entire field can be characterized as a significantly affected area,moderately affected area,or non-affected area.Working face 6106 in the Chuancao Gedan Mine had a coal bed Jepth <80 m and was located in what was identified as a significantly affected area.Hence,mining may cause sliding of the gully slope and increased loading(including significant dynamic loading) on the roof strata.Field tests suggest that significant dynamic pressures were observed at the body and foot of the gully slope,and that dynamic loadings were observed upslope of the working face expansion,provided that the expanding direction of the working face is parallel to the gully. | Li Jianwei Liu Changyou Zhao Tong | 2016 | International Journal of Mining Science and Technology2016，26，2： | 6 |
| 15 | Effects of caving–mining ratio on the coal and waste rocks gangue flows and the amount of cyclically caved coal in fully mechanized mining of super-thick coal seams显示文摘Aimed at determining the appropriate caving–mining ratio for fully mechanized mining of 20 m thick coal seam, this research investigated the effects of caving–mining ratio on the flow fields of coal and waste rocks, amount of cyclically caved coal and top coal loss by means of numerical modeling. The research was based on the geological conditions of panel 8102 in Tashan coal mine. The results indicated the loose coal and waste rocks formed an elliptical zone around the drawpoint. The ellipse enlarged with decreasing caving–mining ratio. And its long axis inclined to the gob gradually became vertical and facilitating the caving and recovery of top coal. The top coal loss showed a cyclical variation; and the loss cycle was shortened with the decreasing in caving–mining ratio. Moreover, the mean squared error(MSE) of the amount of cyclically caved coal went up with increasing caving–mining ratio, indicating a growing imbalance of amount of cyclically caved coal, which could impede the coordinated mining and caving operations. Finally it was found that a caving–mining ratio of 1:2.51 should be reasonable for the conditions. | Zhang Ningbo Liu Changyou Pei Mengsong | 2015 | International Journal of Mining Science and Technology2015，25，1： | 6 |
| 16 | Recent advances in cell imaging and cytotoxicity of intracellular stimuli-responsive nanomaterials显示文摘The stimuli-responsive nanomaterials are gaining more and more interest in the biological field,including cell imaging and biosensing etc. Nanomaterials in response to the bio-relevant stimuli(i.e., p H, enzymes and other bioactive molecules) can be utilized to enhance imaging(i.e., optical imaging, MRI, and multi-mode imaging) sensitivity via disease site-specific delivery and controlled release, which helps to diagnose cancer at an early stage or to monitor progression during treatment. In the triggered responsive process, smart nanomaterials undergo changes in physiochemical properties that can cause cytotoxicity or influence on cell functions due to the interactions between nanomaterials and cells. In order to promote the design and fabrication of effective platforms for therapeutics and diagnostics, special attention should be paid to these effects. By taking the advantages of intracellular stimuli, the controlled self-assembly in living cells can be achieved, which has been used for various in situ detections and insights into biological self-assembly. In this review, the recent advances in cell imaging, cytotoxicity and self-assembly of intracellular stimuli-responsive nanomaterials are summarized. Some principles for the further design and applications of intracellular stimuli-responsive nanomaterials and future perspectives are discussed. | Wenbo Zhang Changyou Gao | 2015 | Science Bulletin2015，60，23： | 6 |
| 17 | Reliability-based Maintenance Optimization under Imperfect Predictive Maintenance显示文摘The reliability-based maintenance optimization model has been focused by the engineers and scholars but it has never been solved effectively to formulate the effect of a maintenance action on the optimization model.In existing works,the system reliability was assumed to be increased to 1 after a predictive maintenance.However,it is very difficult in the most practical systems.Therefore,a new reliability-based maintenance optimization model under imperfect predictive maintenance (PM) is proposed in this paper.In the model,the system reliability is only restored to Ri (0 | LI Changyou ZHANG Yimin XU Minqiang | 2012 | Chinese Journal of Mechanical Engineering2012，25，1： | 6 |
| 18 | Bone tissue regeneration:The role of finely tuned pore architecture of bioactive scaffolds before clinical translation显示文摘Spatial dimension of pores and interconnection in macroporous scaffolds is of particular importance in facilitating endogenous cell migration and bone tissue ingrowth.However,it is still a challenge to widely tune structure parameters of scaffolds by conventional methods because of inevitable pore geometrical deformation and poor pore interconnectivity.Here,the long-term in vivo biological performances of nonstoichiometric bioceramic scaffolds with different pore dimensions were assessed in critical-size femoral bone defect model.The 6%Mg-substituted wollastonite(CSi-Mg6)powders were prepared via wet-chemical precipitation and the scaffolds elaborately printed by ceramic stereolithography,displaying designed constant pore strut and tailorable pore height(200,320,450,600μm),were investigated thoroughly in the bone regeneration process.Together with detailed structural stability and mechanical properties were collaboratively outlined.BothμCT and histological analyses indicated that bone tissue ingrowth was retarded in 200μm scaffolds in the whole stage(2-16 weeks)but the 320μm scaffolds showed appreciable bone tissue in the center of porous constructs at 6-10 weeks and matured bone tissue were uniformly invaded in the whole pore networks at 16 weeks.Interestingly,the neo-tissue ingrowth was facilitated in the 450μm and 600μm scaffolds after 2 weeks and higher extent of bone regeneration and remodeling at the later stage.These new findings provide critical information on how engineered porous architecture impact bone regeneration in vivo.Simultaneously,this study shows important implications for optimizing the porous scaffolds design by advanced additive manufacture technique to match the clinical translation with high performance. | Ronghuan Wu Yifan Li Miaoda Shen Xianyan Yang Lei Zhang Xiurong Ke Guojing Yang Changyou Gao Zhongru Gou Sanzhong Xu | 2021 | Bioactive Materials2021，6，5： | 5 |
| 19 | Dexamethasone-loaded ROS-responsive poly(thioketal)nanoparticles suppress inflammation and oxidative stress of acute lung injury显示文摘Acute lung injury(ALI)is associated with excessive inflammatory response,leading to acute respiratory distress syndrome(ARDS)without timely treatment.A fewer effective drugs are available currently to treat the ALI/ARDS.Herein,a therapeutic nanoplatform with reactive oxygen species(ROS)-responsiveness was developed for the regulation of inflammation.Dexamethasone acetate(Dex)was encapsulated into poly(thioketal)polymers to form polymeric nanoparticles(NPs)(PTKNPs@Dex).The NPs were composed of poly(1,4-phenyleneacetonedimethylene thioketal)(PPADT)and polythioketal urethane(PTKU),in which the thioketal bonds could be cleaved by the high level of ROS at the ALI site.The PTKNPs@Dex could accumulate in the pulmonary inflammatory sites and release the encapsulated payloads rapidly,leading to the decreased ROS level,less generation of pro-inflammatory cytokines,and reduced lung injury and mortality of mice.RNA sequencing(RNA-seq)analysis showed that the therapeutic efficacy of the NPs was associated with the modulation of many immune and inflammation-linked pathways.These findings provide a newly developed nanoplatform for the efficient treatment of ALI/ARDS. | Zihe Zhai Wei Ouyang Yuejun Yao Yuqi Zhang Haolan Zhang Feng Xu Changyou Gao | 2022 | Bioactive Materials2022，7，8： | 5 |
| 20 | Doxorubicin-conjugated pH-responsive gold nanorods for combined photothermal therapy and chemotherapy of cancer显示文摘Cancer chemotherapy can be hindered by drug resistance which leads to lower drug efficiency.Here,we have developed a drug delivery system that tethers doxorubicin to the surface of gold nanorods via a pHsensitive linkage(AuNRs@DOX),for a combined photothermal and chemical therapy for cancer.First,AuNRs@DOX is ingested by HepG2 liver cancer cells.After endocytosis,the acidic pH triggers the release of doxorubicin,which leads to chemotherapeutic effects.The gold nanorods are not only carriers of DOX,but also photothermal conversion agents.In the presence of an 808 nm near-infrared laser,AuNRs@DOX significantly enhance the cytotoxicity of doxorubicin via the photothermal effect,which induces elevated apoptosis of hepG2 cancer cells,leading to better therapeutic effects in vitro and in vivo. | Jin Chen Xiao Li Xinlian Zhao QianQian Wu Huihui Zhu Zhengwei Mao Changyou Gao | 2018 | Bioactive Materials2018，3，3： | 5 |
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