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| 1 | 胡萝卜软腐果胶杆菌巴西亚种(Pectobacterium carotovorum subsp.brasiliense)引起的黄瓜细菌性软腐病在中国发生显示文摘2014—2015年,在中国山东、山西、河北、河南、辽宁等省的黄瓜茎叶上发生了毁灭性的细菌性软腐病,造成了黄瓜产业严重的经济损失。黄瓜叶片、茎、叶柄和果实表面出现流胶;茎基部呈深褐色进而出现湿腐。受侵染的黄瓜叶边缘出现黄斑和湿腐症状,并逐渐扩散至叶片中心。从受侵染组织中分离出45个细菌菌株,在形态学特性鉴定、生理学、生物化学和16Sr RNA基因序列分析的基础上,将病原体鉴定为胡萝卜软腐果胶杆菌(Pectobacterium carotovorum)。多位点序列分析(MLSA)证实,分离菌株是胡萝卜软腐果胶杆菌巴西亚种[Pectobacterium carotovorum subsp.brasiliensis(Pcb)],病原菌归入进化枝II。重新分离获得与原始菌株相同的菌株,证实了分离菌株的致病力。寄主范围测试表明,该菌株具有广泛的寄主范围。目前为止,这是对Pcb引起的黄瓜软腐病——这一对黄瓜生产具有重要经济影响的病害在中国乃至全球范围内的首次报道。 | 冯志琴(译) meng x l chai a l shi y x xie x w ma z h li b j | 2017 | 中国植保导刊2017，37，7： | 10 |
| 2 | Consensus on the digestive endoscopic tunnel technique显示文摘With the digestive endoscopic tunnel technique(DETT), many diseases that previously would have been treated by surgery are now endoscopically curable by establishing a submucosal tunnel between the mucosa and muscularis propria(MP). Through the tunnel, endoscopic diagnosis or treatment is performed for lesions in the mucosa, in the MP, and even outside the gastrointestinal(GI) tract.At present, the tunnel technique application range covers the following:(1)Treatment of lesions originating from the mucosal layer, e.g., endoscopic submucosal tunnel dissection for oesophageal large or circular early-stage cancer or precancerosis;(2) treatment of lesions from the MP layer, per-oral endoscopic myotomy, submucosal tunnelling endoscopic resection, etc.; and(3) diagnosis and treatment of lesions outside the GI tract, such as resection of lymph nodes and benign tumour excision in the mediastinum or abdominal cavity. With the increasing number of DETTs performed worldwide, endoscopic tunnel therapeutics, which is based on DETT, has been gradually developed and optimized. However, there is not yet an expert consensus on DETT to regulate its indications, contraindications, surgical procedure, and postoperative treatment.The International DETT Alliance signed up this consensus to standardize the procedures of DETT. In this consensus, we describe the definition, mechanism,and significance of DETT, prevention of infection and concepts of DETTassociated complications, methods to establish a submucosal tunnel, and application of DETT for lesions in the mucosa, in the MP and outside the GI tract(indications and contraindications, procedures, pre-and postoperative treatments, effectiveness, complications and treatments, and a comparison between DETT and other operations). | Ning-Li Chai Hui-Kai Li En-Qiang Linghu Zhao-Shen Li Shu-Tian Zhang Yu Bao Wei-Gang Chen Philip WY Chiu Tong Dang Wei Gong Shu-Tang Han Jian-Yu Hao Shui-Xiang He Bing Hu1 Bing Hu2 Xiao-Jun Huang Yong-Hui Huang Zhen-Dong Jin Mouen A Khashab James Lau Peng Li Rui Li De-Liang Liu Hai-Feng Liu Jun Liu Xiao-Gang Liu Zhi-Guo Liu Ying-Cai Ma Gui-Yong Peng Long Rong Wei-Hong Sha Pateek Sharma Jian-Qiu Sheng Shui-Sheng Shi Dong Wan Seo Si-Yu Sun Gui-Qi Wang Wen Wang Qi Wu Hong Xu Mei-Dong Xu Ai-Ming Yang Fang Yao Hong-Gang Yu Ping-Hong Zhou Bin Zhang Xiao-Feng Zhang Ya-Qi Zhai | 2019 | World Journal of Gastroenterology2019，25，7： | 5 |
| 3 | Prohibitin(PHB)interacts with AKT in mitochondria to coordinately modulate sperm motility显示文摘Prohibitin(PHB),an evoluti on arily con served mitochondrial inner membra ne protein,is highly expressed in cells that require strong mitoch on drial function.Recently,we dem on strated that the deleti on of Phb in spermatocytes results in impaired mitochondrial function.In addition,PHB expression in the mitochondrial sheath of human sperm has a significantly negative correlation with mitochondrial reactive oxygen species levels,but a positive one with mitochondrial membrane potential and sperm motility.These results suggest that mitochondrial PHB expression plays a role in sperm motility.However,the mechanism of PHB-mediated regulation of sperm motility remai ns unk nown.Here,we dem on strate for the first time that PHB interacts with protei n kinase B(AKT)and exists in a complex with phospho-PHB(pT258)and phospho-AKT in the mitochondrial sheath of murine sperm,as determined using colocalization and coimmunoprecipitation assays.After blocking AKT activity using wortmannin(a phosphatidylinositol 3-kinase[PI3K]inhibitor),murine sperm have significantly(P<0.05)decreased levels of phospho-PHB(pT258)and the total and progressive motility.Furthermore,significantly(P<0.05)lower levels of phospho-PI3K P85 subunit a+γ(pY199 and pY46)and phospho-AKT(pS473;pT308)are found in sperm from infertile asthenospermic and oligoasthenospermic men compared with no rmospermic subjects,which suggest a reduced activity of the PI3K/AKT pathway in these in fertile subjects.Importantly,these sperm from infertile subjects also have a significantly(P<0.05)lower level of phospho-PHB(pT258).Collectively,our findings suggest that the interaction of PHB with AKT in the mitochondrial sheath is critical for sperm motility,where PHB phosphorylation(pT258)level and PI3K/AKT activity are key regulatory factors. | Xiao-Hui Li Ran-Ran Chai Guo-Wu Chen Ling-Fei Zhang Wen-Jing Tan-Tai Hui-Juan Shi Patricia A Martin-DeLeon Wai-Sum O Hong Chen | 2020 | Asian Journal of Andrology2020，22，6： | 3 |
| 4 | The application of bioflocculant for the removal of humic acids from stabilized landfill leachates显示文摘 | Anastasios I Zouboulis Xiao-Li Chai Ioannis A Katsoyiannis | 2004 | Journal of Environmental Management2004，，1： | 2 |
| 5 | Modeling of soil-pile-superstructure interaction in the design of bridge foundations显示文摘 | Chai J | 1995 | Geotechnical Special Publication1995，51，： | 1 |
| 6 | Discovery and analysis of inflammatory disease releted genes using cDNA microarray显示文摘 | Schena M Chai A | 1997 | Proc Natl Acad Sci USA1997，94，6： | 1 |
| 7 | Ovarian tissue cryopreservation by vitrification in olive baboons (papio anu- bis) : a pilot study显示文摘 | Nyachieo A Spiessens C Chai DC | 2013 | Gynecol Obstet Invest2013，75，3： | 1 |
| 8 | Synthesis and characterization of graphene and carbon nanotubes:A review on the past and recent developments 显示文摘 | Liu W W Chai S P Mohamed A R | 2014 | Journal of Industrial and Engineering Chemistry2014，20，： | 1 |
| 9 | Discovery and analysis of inflammatory disease-related genes using cDNA microarray 显示文摘 | Schena M Chai A | 2000 | Nature2000，404，4： | 1 |
| 10 | Submaximal effort tolerance as a predictor of all-cause mortality in patients undergoing cardiac reha bilitation显示文摘 | Feuerstadt P Chai A Kligfield P | 2007 | Clin Cardiol2007，30，5： | 1 |
| 11 | Effects of inhaled corticosteroid on bone turnover in children with bronchial asthma显示文摘 | Chay OM Goh A Lim WH | 1999 | Respirology1999，4，1： | 1 |
| 12 | Discovery and analysis of inflammatory disease- related genes using cDNA microarrays显示文摘 | Hler R A Schena M Chai A | 1997 | Proc Natl Acad Sci USA1997，94，6： | 1 |
| 13 | Discovery and analysis of inflammatory disease-related genes using cDNA microarrays 显示文摘 | Heller RA Schena M Chai A | 1997 | Proce Natl Acad Sei USA1997，94，6： | 1 |
| 14 | The application of bioflocculant for the removal of humic acids from stabilized landfill leachates 显示文摘 | Zouboulis A 1 Chai X L Katsoyiannis I A | 2004 | J Environ Manag2004，70，： | 1 |
| 15 | Telomere- end processing the terminal nucleotides of human chromosomes 显示文摘 | Sfeir A J Chai W Shay J W | 2005 | Mol Cell2005，18，1： | 1 |
| 16 | Ensem- ble-based chemical data assimilation, h general ap- proach 显示文摘 | Constantinescu E M Sandu A Chai T | 2007 | Quarterly Journal of the Royal Meteoro- logical Society2007，133，626： | 1 |
| 17 | Performance of wheat/maize intercropping is a function of belowground interspecies interactions显示文摘 | Mu Y P Chai Q Yu A Z | 2013 | Crop Science2013，53，5： | 1 |
| 18 | Discovery and analysis of inflammatory disease-related genes using cDNA microarrays显示文摘 | Heller RA Schena M Chai A | 1997 | Proc Natl Acad Sci USA1997，94，： | 1 |
| 19 | Clinical presentation and disease associations of gout: a hospital-based study of 100 patients in Singapore显示文摘 | Koh WH Seah A Chai P | 1998 | Ann Aead Med Singapore1998，27，1： | 1 |
| 20 | Influence of malic acid supplementation on ruminal p H, lactic acid utilization, and digestive function in steers fed high-concentrate finishing diets显示文摘 | Montafio M F Chai W Zinn-Ware T E et a l | 1999 | Journal of Animal Science1999，77，3： | 1 |
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