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| 1 | Thiopurine-methyltransferase variants in inflammatory bowel disease:Prevalence and toxicity in Brazilian patients显示文摘AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. | Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza | 2014 | World Journal of Gastroenterology2014，20，12： | 3 |
| 2 | 日粮中添加氨基酸降低粗蛋白含量对6~15kg仔猪生产性能的影响显示文摘为评价添加氨基酸降低粗蛋白(CP)含量对6~15 kg仔猪的影响,共设计两个试验。在性能试验中(试验Ⅰ),21日龄、初始体重为5.95±0.33 kg的断奶仔猪120头被分成5组。试验为完全随机区组试验设计,共8个重复,每个重复3只猪。在5个处理组中,CP含量由21.0%降低到15.0%(分别为21.0%,19.5%,18.0%,16.5%和15.0%),通过添加L-赖氨酸、DL-蛋氨酸、L-苏氨酸、L-色氨酸、L-缬氨酸和L-异亮氨酸来满足日粮中氨基酸的需求。各处理间生产性能无显著差异。因此,以上蛋白质的任何水平都能被有效的合成氨基酸。降低蛋白质的含量可以增加必需氨基酸和非必需氨基酸的比值,且蛋白质含量为15%时比值最佳(5347)。随着蛋白质含量的降低,尿素含量线性下降(试验Ⅰ)。为评估氮平衡(试验Ⅱ),21日龄的杂交阉割商品仔猪20只被随机分成5组,仔猪的平均活重为10.79±2.19kg。5个处理组的所有动物安置在铁笼中,每个处理4个重复,每个重复1只猪。结果血液和尿液中尿素含量随蛋白质含量的减少线性降低(P〈0.05),进而减少了动物向环境中的氮排泄。 | Toledo J B Furlan A C Pozza P C Pianob L M Carvalho P L O Peuela-Sierra L M Huepa L M D | 2014 | 饲料博览2014，0，11： | 3 |
| 3 | Use of siRNA molecular beacons to detect and attenuate mycobacterial infection in macrophages显示文摘Tuberculosis is one of the leading infectious diseases plaguing mankind and is mediated by the facultative pathogen, Mycobacterium tuberculosis(MTB). Once the pathogen enters the body, it subverts the host immune defenses and thrives for extended periods of time within the host macrophages in the lung granulomas, a condition called latent tuberculosis(LTB). Persons with LTB are prone to reactivation of the disease when the body's immunity is compromised. Currently there are no reliable and effective diagnosis and treatment options for LTB, which necessitates new research in this area. The mycobacterial proteins and genes mediating the adaptive responses inside the macrophage is largely yet to be determined. Recently, it has been shown that the mce operon genes are critical for host cell invasion by the mycobacterium and for establishing a persistent infection in both in vitro and in mouse models of tuberculosis. The Yrb E and Mce proteins which are encoded by the MTB mce operons display high degrees of homology to the permeases and the surface binding protein of the ABC transports, respectively. Similarities in structure and cell surface location impute a role in cell invasion at cholesterol rich regions and immunomodulation. The mce4 operon is also thought to encode a cholesterol transport system that enables the mycobacterium to derive both energy and carbon from the host membrane lipids and possibly generating virulence mediating metabolites, thus enabling the bacteria in its long term survival within the granuloma. Various deletion mutation studies involving individual or whole mce operon genes have shown to be conferring varying degrees of attenuation of infectivity or at times hypervirulence to the host MTB, with the deletion of mce4 A operon gene conferring the greatest degree of attenuation of virulence. Antisense technology using synthetic si RNAs has been used in knocking down genes in bacteria and over the years this has evolvedinto a powerful tool for elucidating the roles of various genes mediating infectivity and survival in mycobacteria. Molecular beacons are a newer class of antisense RNA tagged with a fluorophore/quencher pair and their use for in vivo detection and knockdown of mR NA is rapidly gaining popularity. | Remo George Renata Cavalcante Celso Carvalho Jr Elyana Marques Jonathan B Waugh M Tino Unlap | 2015 | World Journal of Experimental Medicine2015，5，3： | 2 |
| 4 | Measurement of hepatic glucose output,krebs cycle,and gluconeogenic fluxes by NMR analysis of a single plasma glucose sample显示文摘 | Jones JG Carvalho RA Franco B | 1998 | Anal Biochem1998，263，1： | 1 |
| 5 | An inverse scattering series method for attenuating multiples in seismic reflection data显示文摘 | Weglein A B Gasparotto F A Carvalho P M Stolt R H | 1997 | Geophysics1997，62，： | 1 |
| 6 | Concurrent hypermethylation ofgene promoters is associated with a MSI-H phenotype and diploidyin gastric carcinomas显示文摘 | Carvalho B Pinto M Cimes L | 2003 | Eur J Cancer2003，39，9： | 1 |
| 7 | Intracellular signaling mechanisms in photodynamic therapy显示文摘 | Almeida RD Manadas B J Carvalho AP | 2004 | Biochem Bio- phys Acta2004，1704，2： | 1 |
| 8 | Effects of herbivory by Hyblaea puera ( Hyblaeidae : Lepidop- tera) on litter production in the mangrove on the coast of Brazi- lian Amazonia显示文摘 | FERNANDES M E B NASCIMENTO A A M CARVALHO M L | 2009 | Journal of Tropical Ecology2009，25，3： | 1 |
| 9 | BAC to the future or oligonucleotides:a perspective for microarray comparative genomic hybridization (array CGH)显示文摘 | Ylstra B van den Ijssel P Carvalho B | 2006 | Nucl Acid Res2006，34，： | 1 |
| 10 | The chemistry of Brazilian Myristieaceae显示文摘 | Fo R B De Carvalho M G Gottlieb O R | 1984 | Planta Med1984，50，1： | 1 |
| 11 | Sediment phosphorus cycling in a large shallow lake: spatio-temporal variation phosphorus pools and release显示文摘 | Spears B M Carvalho L Perkins R | 2007 | Hydrobiologia2007，584，1： | 1 |
| 12 | A symbolic fault-prediction model based on multiobjective particle swarm optimization 显示文摘 | De Carvalho A B Pozo A Vergilio S R | 2010 | Journal of Systems and Software2010，83，5： | 1 |
| 13 | Reduction in the length of stay with rivaroxaban as a single-drug regimen for the treatment of deep vein thrombosis and pulmonary embolism显示文摘 | van Bellen B Bamber L Correa de Carvalho F | 2014 | Curr Med Res Opin2014，30，： | 1 |
| 14 | Insufficient cure under the condition of highirradiance and short irradiation time 显示文摘 | Feng L Carvalho R Suh B I | 2009 | Dent Mater2009，25，3： | 1 |
| 15 | High resolution microarray comparative genomic hybridisation analysis using spotted oligonucleotides显示文摘 | Carvalho B Ouwerkerk E Meijer G A | 2004 | J Clin Pathol2004，57，6： | 1 |
| 16 | Maternal anxiety and depression and development of prematurely horn infants in the first year of life显示文摘 | Carvalho A E Martinez F E Linhares M B | 2008 | Span J Psychol2008，11，2： | 1 |
| 17 | Oxytocin for labour and caesarean deliveryimplications for the anaesthesiolo-gist显示文摘 | Dyer RA Butwick AJ Carvalho B | 2011 | Curr Opin Anaesthesiol2011，24，3： | 1 |
| 18 | Filling lateral canals: evaluation of different filling techniques 显示文摘 | Carvalho-Sousa B Almeida-Gomes F Carvalho PR | 2010 | Eur J Dent2010，4，3： | 1 |
| 19 | Aneuploidies detection in miscarriages and fetal deaths using multiplex ligation-dependent probe amplification:an alternative for speeding up results?显示文摘 | Carvalho B Doria S Ramalho C | 2010 | Eur J Obstet Gynecol Reprod Biol2010，153，2： | 1 |
| 20 | Algorithms for fuzzy segmentation 显示文摘 | CARVALHO B M GAU C J HERMAN G T | 1999 | Pattern Analysis & Applications1999，2，1： | 1 |
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