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| 1 | MicroRNA profile in neosquamous esophageal mucosa following ablation of Barrett's esophagus显示文摘AIM To investigate the micro RNA expression profile in esophageal neosquamous epithelium from patients who had undergone ablation of Barrett's esophagus.METHODS High throughput screening using Taq Man~ Array Human Micro RNA quantitative PCR was used to determine expression levels of 754 micro RNAs in distal esophageal mucosa(1 cm above the gastro-esophageal junction) from 16 patients who had undergone ablation of non-dysplastic Barrett's esophagus using argon plasma coagulation vs pretreatment mucosa, posttreatment proximal normal non-treated esophageal mucosa, and esophageal mucosal biopsies from 10 controls without Barrett's esophagus. Biopsies of squamous mucosa were also taken from 5 cm above the pre-ablation squamo-columnar junction. Predicted m RNA target pathway analysis was used to investigate the functional involvement of differentially expressed micro RNAs.RESULTS Forty-four micro RNAs were differentially expressed between control squamous mucosa vs post-ablation neosquamous mucosa. Nineteen micro RNAs were differentially expressed between post-ablation neosquamous and post-ablation squamous mucosa obtained from the more proximal non-treated esophageal segment. Twelve microRNAs were differentially expressed in both neosquamous vs matched proximal squamous mucosa and neosquamous vs squamous mucosa from healthy patients. Nine micro RNAs(mi R-424-5p, mi R-127-3p, mi R-98-5p, mi R-187-3p, mi R-495-3p, mi R-34c-5p, mi R-223-5p, mi R-539-5p, mi R-376a-3p, mi R-409-3p) were expressed at higher levels in post-ablation neosquamous mucosa than in matched proximal squamous and healthy squamous mucosa. These micro RNAs were also more highly expressed in Barrett's esophagus mucosa than matched proximal squamous and squamous mucosa from controls. Target prediction and pathway analysis suggests that these micro RNAs may be involved in the regulation of cell survival signalling pathways. Three micro RNAs(mi R-187-3p, mi R-135b-5p and mi R-31-5p) were expressed at higher levels in postablation neosquamous mucosa than in matched proximal squamous and healthy squamous mucosa. These mi RNAs were expressed at similar levels in preablation Barrett's esophagus mucosa, matched proximal squamous and squamous mucosa from controls. Target prediction and pathway analysis suggests that these micro RNAs may be involved in regulating the expression of proteins that contribute to barrier function.CONCLUSION Neosquamous mucosa arising after ablation of Barrett's esophagus expresses micro RNAs that may contribute to decreased barrier function and micro RNAs that may be involved in the regulation of survival signaling pathways. | Loveena Sreedharan George C Mayne David I Watson Timothy Bright Reginald V Lord Alfiya Ansar Tingting Wang Jakob Kist David StJ Astill Damian J Hussey | 2017 | World Journal of Gastroenterology2017，23，30： | 3 |
| 2 | The relationship between critical pressure and width of gas vesicles in isolates of Planktothrix rubescens from Lake Zurich 显示文摘 | Bright D I Walsby A E | 1999 | Microbiology1999，145，： | 1 |
| 3 | Me- tabonomics, dietary influences and cultural differences: a 1H NMR-based study of urine samples obtained from healthy British and Swedish subjects显示文摘 | Lenz E M Bright J Wilson I D | 2004 | J Pharm Biomed Anal2004，36，4： | 1 |
| 4 | Acute tolerance to continuously infused alfentanil: the role of cholecystokinin and N-methyl-D-aspartate-nitric oxide systems显示文摘 | Kissin I Bright C A Bradley E L | 2000 | Anesth Analg2000，91，1： | 1 |
| 5 | Cellulose crystallinity change assessment of biochar produced by pyrolysis of coir pith显示文摘 | Rojith G Bright S I | 2012 | Research Journal of Recent Sciences2012，，2： | 1 |
| 6 | Immunostimulatory effect of a marine yeast Candida sake S165 in Fenneropenaeus indicus 显示文摘 | Sajeevan T P Philip R Bright I S | 2006 | Aquaculture2006，，257： | 1 |
| 7 | Surveillance for neuraminidase inhibitor resistance among human influenza A and B viruses circulating worldwide from 2004to 2008显示文摘 | Sheu T G Deyde V M Okomo-adhiambo M Garten R J Xu X Bright R A Butler E N Wallis T R Klimov A I Gubareva L V | 2008 | Antimicrob Agents Chemother2008，52，9： | 1 |
| 8 | Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus显示文摘BACKGROUND Circulating microRNAs(miRNAs)are potential biomarkers for many diseases.However,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA biomarkers.While small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been defined.AIM To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker studies.METHODS Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano Sight.RNA was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q PCR.The overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV preparations.The diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also compared.RESULTS The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,P<0.0001)between the plasma and serum s EV preparations,but was consistently higher in the plasma s EV preparations.Specific blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(P<0.05).In plasma s EV preparations,the percentage of protein-associated mi RNAs expressed at relatively higher levels(Ct 20-25)was greater than serum s EV preparations(50%vs 31%).While the percentage of vesicle-associated mi RNAs expressed at relatively higher levels was greater in the serum s EV preparations than plasma s EV preparations(70%vs 44%).A 5-mi RNA biomarker panel produced a higher cross validated accuracy for discriminating patients with esophageal adenocarcinoma from healthy controls using serum s EV preparations compared with plasma s EV preparations(AUROC 0.80 vs 0.54,P<0.05).CONCLUSION Although plasma s EV preparations contained more mi RNAs than serum s EV preparations,they also contained more mi RNAs from non-vesicle origins.Serum appears to be more suitable than plasma for s EV mi RNAs biomarkers studies. | Karen Chiam George C Mayne Tingting Wang David I Watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball Joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian J Hussey | 2020 | World Journal of Gastroenterology2020，26，20： | 1 |
| 9 | Caninflammatory pain prevent the development of acute tolerance to alfentanil显示文摘 | Kissin I Bright CA Bradley EL Jr | 2001 | Anesth Analg2001，92，5： | 1 |
| 10 | Pore - throat size distributions in permo- triassic sandstones from the United Kingdom and some implications for con- taminant hydrogeology 显示文摘 | Bloomfield J P Gooddy D C Bright M I | 2001 | Hydrogeology Journal2001，9，3： | 1 |
| 11 | Identifying fractions on number lines显示文摘 | Bright G.W Behr M.J Post T.R Wachsmuth I | | 0，，03： | 1 |
| 12 | Development of a cell culture system from the ovarian tissue of African catfish (Clarias gariepinus) 显示文摘 | Sunil K G Bright S I S Philip R | 2001 | Aquaculture2001，194，： | 1 |
| 13 | A lH-NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects 显示文摘 | Lenz E M Bright J Wilson I D | 2003 | J Pharm Biomed Anal2003，33，5： | 1 |
| 14 | The relationship between critical pressure and width of gas vesicles in isolates of Planktothrix rubescens from Lake Zrich 显示文摘 | Bright D I Walsby A E 1999 | 1999 | Microbiology1999，145，： | 1 |
| 15 | Acute tolerance to continuously infused alfentanil:the role of cholecystokinin and N-methyl-D-aspartate-nitric oxide systems显示文摘 | KISSIN I BRIGHT C A BRADLEY E L J R | 2000 | Anesth Analg2000，91，1： | 1 |
| 16 | The effect of ketamine on opioid- induced acute tolerance : can it explain reduction of opioid consumption with ketamine-opioid analgesic combination 显示文摘 | Kissin I Bright CA Bradley EL Jr | 2000 | Anesth Analg2000，91，6： | 1 |
| 17 | Dose/frequency:A critical factor in the administration of glucan as immunostimulant to Indian white shrimp Fen-neropenaeus indicus 显示文摘 | Sajeevan T P Philip R Bright Singh I S | 2009 | Aquaculture2009，287，34： | 1 |
| 18 | A 1H NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects 显示文摘 | Lenz E M Bright J Wilson I D | 2003 | J Pharm Biomed Anal2003，33，5： | 1 |
| 19 | A ^1H-NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects显示文摘 | E M Lenz J Bright I D Wilson | 2003 | J Pharm Biomed Anal2003，33，： | 1 |
| 20 | Immunostimulatory effect of a marine yeast Candida sake S165 in Fenneropenaeus indicus显示文摘 | Sajeevan T P Rosamma P Bright S I S | 2006 | Aquaculture2006，257，： | 1 |
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